LIU Ziyan,LI Dan,ZHU Yuanyuan,et al.Physiological Effects of Exogenous SA on Low Temperature Stress of Walnut Annual Branches[J].Northern Horticulture,2022,(13):25-34.[doi:10.11937/bfyy.20214979]
外源SA对核桃一年生枝低温胁迫的生理影响
- Title:
- Physiological Effects of Exogenous SA on Low Temperature Stress of Walnut Annual Branches
- Keywords:
- salicylic acid; walnut; annual branch; phloem; low temperature stress; physiological index
- 文献标志码:
- A
- 摘要:
- 以河北省临城县河北绿岭公司核桃示范园基地十三年生‘绿岭’核桃一年生枝为试材，采用室内低温模拟试验、室内分析等方法，研究了喷施不同浓度SA对不同越冬期核桃一年生枝相对电导率、韧皮部抗氧化酶活性、丙二醛（MDA）含量、渗透调节物质的影响，以期为核桃树越冬管理提供参考依据。结果表明：低温胁迫导致核桃一年生枝相对电导率（REC）升高，韧皮部MDA、脯氨酸（Pro）、可溶性蛋白质（SP）含量增加，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性在一定范围内增强；无论室内低温，还是大田自然低温，各浓度SA处理在一定程度上降低核桃一年生枝REC，降低枝条韧皮部MDA含量，提高SP、Pro含量，提高SOD、POD、CAT活性。与CK相比，喷施SA能显著（P<0.05）增强核桃一年生枝抗寒性，在-30 ℃时，SA1处理的枝条韧皮部的MDA含量为8.62 μmol?g-1，较CK降低12.55%；SOD、CAT、POD活性和SP、Pro含量分别为0.96 U?g-1?min-1FW、13.47 U?g-1?min-1FW、5.46 U?g-1?min-1FW和17.28 μg?g-1、56.25 μg?g-1，分别较CK升高7.07%、359.10%、68.36%、6.35%、54.98%。各处理抗低温胁迫的能力综合表现结果为SA1＞SA5＞SA0.25＞SA10＞CK，即外源SA处理对增强核桃一年生枝抗寒性能力以1 mmol?L-1 SA效果最佳。
- Abstract:
- Taking the 13-year-old ‘Lyuling’ walnut annual branches in the walnut demonstration garden base of Hebei Lyuling company in Lincheng county,Hebei Province as test materials,lab low temperature simulation test and lab analysis were used to study the effects of spraying SA on the transcriptome,antioxidant enzyme activity,MDA content,relative conductivity and osmotic adjustment substances on the cold resistance of walnut annual branches by natural overwintering and artificial low temperature regulation,in order to provide reference for walnut overwintering management.The results showed that low temperature stress increased REC of annual branches and MDA,Pro and SP contents in phloem and enhanced the activities of SOD,POD and CAT in a certain range.Regardless of artificial low temperature or natural low temperature,each SA treatment decreased REC of annual branches and the contents of MDA content in phloem,SP and Pro contents and SOD,POD and CAT activities of walnut annual branches to a certain extent.Compared with the CK.At -30 ℃,the MDA content in phloem of branches treated with SA1 was 8.62 μmol?g-1,12.55% lower than the CK.The activities of SOD,POD,CAT and the contents of SP and Pro were 0.96 U?g-1?min-1FW,13.47 U?g-1?min-1FW,5.46 U?g-1?min-1FW and 17.28 μg?g-1,56.25 μg?g-1,respectively increased by 7.07%,359.10%,68.36%,6.35% and 54.98% compared with the CK.The comprehensive results of resistance to low temperature stress of each treatment were as follows,SA1>SA5>SA0.25>SA10>CK,among which the effect of spraying 1 mmol?L-1 SA solution on the leaf was the best.
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备注/Memo
第一作者简介：刘紫烟（1997-），女，硕士研究生，研究方向为森林培育。E-mail：1197545543@qq.com.责任作者：齐国辉（1969-），女，博士，教授，现主要从事经济林研究等工作。E-mail：bdqgh@sina.com.基金项目：河北省创新能力提升计划资助项目（20536807D）。收稿日期：2021-12-07