KANG Chao,ZENG Weijun,ZHENG Xuan,et al.Changes of TTC-Dehydrogenase and Extra-cellular Enzyme Activities for Morchella sextelata Mycelium[J].Northern Horticulture,2022,(05):119-124.[doi:10.11937/bfyy.20212975]
羊肚菌菌丝体TTC-脱氢酶及胞外酶活性变化
- Title:
- Changes of TTC-Dehydrogenase and Extra-cellular Enzyme Activities for Morchella sextelata Mycelium
- 文献标志码:
- A
- 摘要:
- 以六妹羊肚菌为试材，分别测定了菌丝TTC-脱氢酶、羧甲基纤维素酶、淀粉酶、愈创木酚氧化酶活性及残糖含量，研究了羊肚菌不同生长期内菌丝活力及代谢情况，以期为羊肚菌及其它食用菌菌种生产提供参考依据。结果表明：整个培养期内5种酶活性表现一定的规律性，均呈先升后降的趋势。不同酶活性间存在一定相关性，即菌丝活力最强，菌丝生长快，以4~12 d为最佳培养时间，可作为液体菌种生产的最佳时期。
- Abstract:
- Taking Morchella sextelata as test material,the activities of mycelial TTC-dehydrogenase,carboxymethyl cellulase,amylase,guaiacol oxidase,and residual reducing sugar content were investigated by liquid-state culture,the mycelial activity and metabolism of M.sextelata in different growth phase were studied,in order to provide an effective way for detection of mycelial activity and fermentation management with Morchella spp.and other edible fungi.The results showed that the activity changes of the five enzymes rised first and fell after in growth phase.There was a certain correlation between the activity of different enzymes.The mycelium had the strongest vitality and fast growth rate at 4-12 days,which was the best period for the production of liquid strains.
参考文献/References:
[1]刘桂娟,姚方杰.食用菌菌种质量检测方法概述[C].杭州:第二届全国食用菌中青年专家学术交流会,2008.[2]王稳,朱忠贵,李萍萍.食用菌液体菌种的几种实用检测方法[J].食用菌,2005(1):20-21.[3]林增祥,付永前,曾宪贤.食用菌液体菌种发酵终点的简便检测方法[J].中国食用菌,2006(4):25-26.[4]陈国梁,张向前,周茂林,等.羊肚菌液体培养过程中几种胞外酶活性变化研究[J].食用菌,2009,31(1):8-9.[5]陈立佼,王芳,赵永昌,等.羊肚菌产菌核菌株与不产菌核菌株胞外酶活性比较[J].农业科学与技术,2013(10):1392-1396,1408.[6]李迷,王素英,董世瑞,等.TTC-脱氢酶还原法测定螺旋藻细胞活性的条件优化[J].微生物学杂志,2015,35(6):33-37.[7]RYSSOV-NIELSEN H.Measurement of the inhibition of respiration in activated sludge by a modified determination of the ttc-dehydrogenase activity[J].Water Research,1975,9(12):1179-1185.[8]KLAPWUK A,DRENT J,STEENVOORDEN J.A modified procedure for the TTC-dehydrogenase test in activated-sludge[J].Water Research,1974,8(2):121-125.[9]林清泉,丘雪红,郑壮丽,等.蛹虫草退化菌株的特征研究[J].菌物学报,2010,29(5):670-677.[10]黄春花,郑壮丽,梅彩英,等.蛹虫草TTC-脱氢酶测定方法的优化[J].环境昆虫学报,2011,33(3):321-328.[11]刘淑琴,宫文超,李春燕.TTC法细胞酶活力测试技术在北虫草选育种中应用[J].食用菌,2005(2):12-13.[12]张良,杨云龙.用TTC-脱氢酶活性测定法筛选焦化废水优势菌[J].山西建筑,2008(8):202-203.[13]李红,张敏,肖千明,等.利用TTC-脱氢酶还原法快速检测黑木耳菌丝活力的研究[J].食用菌,2016(4):10-12.[14]沈业寿,卫青,张峻岭.美味侧耳子实体组织的细胞活力[J].中国食用菌,1995(6):33-35.[15]沈业寿,沈业宏.侧耳子实体组织的TTC-脱氢酶活性[J].安徽大学学报(自然科学版),1995,19(4):31-33.[16]沈业寿,沈业宏,胡菊芬.紫孢侧耳子实体组织的TTC-脱氢酶活性[J].中国食用菌,1996(1):31-33.[17]郑旋,康超,杨玲,等.不同培养条件对六妹羊肚菌菌丝形态的影响[J].中国食用菌,2020,39(12):53-62.[18]吕春鹤,孙婷婷,张健,等.几种常见食用菌胞外酶活性测定方法的研究[J].中国林副特产,2013(5):93-95,96.[19]陈国梁,张向前,贺晓龙,等.五种羊肚菌液体培养过程胞外酶活性变化研究[J].北方园艺,2010(10):210-213.[20]马浩,高振江.MTT法检测食用菌液体菌种活力的研究[EB/OL].中国科技论文在线.http://www.paper.edu.cn/releasepaper/content/200605-383.(2006-05-30)[2021-05-30].[21]何国维,李伟光,王瑞雪,等.利用2,3,5-三苯基氯化四氮唑(TTC)测定细胞活力的方法与应用[J].军事医学,2014,38(5):388-391.[22]王勇,高苇,张春祥,等.液体培养条件下绿色木霉Tr9701胞外酶及还原糖动态变化研究[J].华北农学报,2015,30(4):226-230.
相似文献/References:
[1]盖玉红,王文慧,王沥浩,等.人参发状根与栽培人参有效成分的比较研究[J].北方园艺,2013,37(07):166.
GAI Yu-hong,WANG Wen-hui,WANG Li-hao,et al.Study on Comparison of Physiological and Biochemical Composition Between Hair Root of Ginseng and Cultivated Ginseng[J].Northern Horticulture,2013,37(05):166.
[2]陈泽英,普晓兰,林 萍,等.水毛茛组织培养研究[J].北方园艺,2011,35(08):0.[doi:10.11937/bfyy.201108058]
CHEN Ze-yingI,PU Xiao-lan,LIN PingI,et al.Study on Tissue Culture of Batrachium bungei[J].Northern Horticulture,2011,35(05):0.[doi:10.11937/bfyy.201108058]
[3]刘月芹,贺晓龙,赵瑞华,等.提高灵芝液体发酵温度对其活性成分含量的影响[J].北方园艺,2020,44(15):118.[doi:10.11937/bfyy.20194292]
LIU Yueqin,HE Xiaolong,ZHAO Ruihua,et al.Effects of Increasing Liquid Fermentation Temperature of Ganoderma lucidum on Its Active Component Content[J].Northern Horticulture,2020,44(05):118.[doi:10.11937/bfyy.20194292]
[4]康超,郑旋,杨玲,等.响应面法优化六妹羊肚菌液体种培养基配方[J].北方园艺,2022,(02):111.[doi:10.11937/bfyy.20212962]
KANG Chao,ZHENG Xuan,YANG Ling,et al.Optimization of Medium for the Liquid Strain Using Response Surface Methodology (RSM) of Morchella sextelata[J].Northern Horticulture,2022,(05):111.[doi:10.11937/bfyy.20212962]
[5]方磊,裴沪荣,龙力,等.两种羊肚菌真菌性病害的鉴定及其防治药剂筛选[J].北方园艺,2022,(10):110.[doi:10.11937/bfyy.20214608]
FANG Lei,PEI Hurong,LONG Li,et al.Identification of Two Fungal Diseases of Morchella Strains and Screening of Control Agents[J].Northern Horticulture,2022,(05):110.[doi:10.11937/bfyy.20214608]
[6]王虹,郑旋,康超,等.羊肚菌继代培养对菌丝特性及TCC-脱氢酶活性的影响[J].北方园艺,2023,(11):123.[doi:10.11937/bfyy.20223523]
WANG Hong,ZHENG Xuan,KANG Chao,et al.Effects of Repeated Subcultures on Mycelial Characteristics andTTC-dehydrogenase Activity of Morchella sextelata[J].Northern Horticulture,2023,(05):123.[doi:10.11937/bfyy.20223523]
备注/Memo
第一作者简介：康超（1987-），男，硕士，高级工程师，现主要从事食药用菌菌种制备、栽培及加工等研究工作。E-mail:464286916@qq.com.基金项目：2018年度贵州省科技计划资助项目（黔科合支撑[2018]2324号）；2019年度贵州省科研机构服务企业计划资助项目（黔科合服企业[2019]4007-6号）；2019年度贵州省科技计划资助项目（黔科合支撑[2019]2451号-5）；2018年度贵州省科技计划资助项目（黔科合支撑[2018]2322）。收稿日期：2021-07-14