SITU Cheng,YU Tianhua,SONG Yuxin.Comparison on Lignocellulose Degradation of Kiwifruit Branches by Different Edible Fungi[J].Northern Horticulture,2022,(05):111-118.[doi:10.11937/bfyy.20213707]
不同食用菌对猕猴桃枝木质纤维素降解的比较
- Title:
- Comparison on Lignocellulose Degradation of Kiwifruit Branches by Different Edible Fungi
- Keywords:
- edible fungi; kiwifruit branches; lignocellulose; degradation
- 文献标志码:
- A
- 摘要:
- 以香菇、平菇、秀珍菇等7种食用菌为研究对象，通过比较菌丝生长状况、木质纤维素降解酶活性和木质纤维素含量，探讨不同食用菌对猕猴桃枝中木质纤维素的降解能力，以期为进一步利用猕猴桃枝生物质提供参考依据。结果表明：猕猴桃枝可作为7种食用菌栽培的原料，但生长适应性不同，食用菌对猕猴桃枝基质中木质纤维素的降解具有一定的选择性；秀珍菇选择性降解木质素效果最佳，栽培20 d后木质素降解率达35.6%；平菇对基质中纤维素降解作用最好，栽培25 d时纤维素降解率达48.3%；鹿角灵芝对基质中半纤维素降解作用最强，栽培30 d时降解率达68.3%。
- Abstract:
- Taking seven kinds of edible fungi of Lentinus edodes,Pleurotus ostreatus and Pleurotus geesteranus etc as research species,the degradation ability of different edible fungi to lignocellulosic in kiwifruit branches was investigated by comparing mycelial growth,lignocellulosic degradation enzyme activity and lignocellulosic content,in order to provide a foundation for further utilization of biomass in kiwifruit branches.The results showed that kiwifruit branches could be used as raw materials for seven kinds of edible fungi,but the growth adaptability was different,and the edible fungi had selectivity for the degradation of lignocellulose in material of kiwifruit branches.The lignin degradation effect of Pleurotus geesteranus was the best,and the rate of lignin degradation was 35.6% after 20 days of cultivation.Pleurotus ostreatus had the best effect on cellulose degradation,and the rate of cellulose degradation reached 48.3% after 25 days of cultivation.Ganoderma amboinense had the strongest degradation effect on hemicellulose in the material,and the degradation rate reached 68.3% after 30 days of cultivation.
参考文献/References:
[1]何康盈,赖锦晖,徐家豪,等.浅谈食用菌产业的发展[J].种子科技,2019,37(14):140-143.[2]HUANG L,SUN N,BAN L T,et al.Ability of different edible fungi to degrade crop straw[J].AMB Express,2019,9(1):1-6.[3]KACHLISHVILI E,TSIKLAURI N,KHARDZIANI T,et al.Physiological regulation of edible and medicinal higher basidiomycetes lignocellulolytic enzyme activity[J].International Journal of Medicinal Mushrooms,2002,4(2):1-8.[4]高锋,曹旸,何明霞,等.香菇、杏鲍菇与平菇部分胞外酶种类及活性变化规律的对比分析[J].北方园艺,2017(23):199-206.[5]DAVIS J K,SELLO J R.Regulation of genes in streptomyces bacteria required for catabolism of lignin-derived aromatic compounds[J].Applied Microbiology and Biotechnology,2010,86(3):921-929.[6]KOSHY J,NAMBISAN P.Ethanol production from spent substrate of Pleurotus eous [J].International Journal of Applied Biology and Pharmaceutical Technology,2012,3(1):280-286.[7]陈莎,李利.一株高效降解稻草木质素食用菌菌株的筛选[J].北方园艺,2020(19):123-128.[8]WANG S N,QIU Y,ZHU F.Kiwifruit (Actinidia spp.):A review of chemical diversity and biological activities[J].Food Chemistry,2021,350:128469-128559.[9]何科佳,曾斌,徐海,等.蚯蚓处理猕猴桃枝条粉碎物的研究[J].中国南方果树,2020,49(2):97-100.[10]ZHANG C Y,YOU S P,ZHANG J X,et al.An effective in-situ method for laccase immobilization:Excellent activity,effective antibiotic removal rate and low potential ecological risk for degradation products[J].Bioresource Technology,2020,308(14):123371-123381.[11]DING C H,WANG X,LI M X.Evaluation of six white-rot fungal pretreatments on cornstover for the production of cellulolytic and ligninolytic enzymes,reducing sugars,and ethanol[J].Applied Microbiology and Biotechnology,2019,103(14):5641-5652.[12]INOUE S,IGARASHI Y,YONEDA Y,et al.Elimination and detoxification of fungicide miconazole and antidepressant sertraline by manganese peroxidase-dependent lipid peroxidation system[J].International Biodeterioration & Biodegradation,2015,100:79-84.[13]ARCHIBALD F S.A new assay for lignin-type peroxidases employing the dye azure B[J].Applied and Environmental Microbiology,1992,58(9):3110-3116.[14]DING C H,LI M X,HU Y Q.High-activity production of xylanase by Pichia stipitis:Purification,characterization,kinetic evaluation and xylooligosaccharides production[J].International Journal of Biological Macromolecules,2018,117:72-77.[15]HU L B,LUO Y P,CAI B,et al.The degradation of lignin in Phyllostachys heterocycla cv.var.pubescens in ethanol solvothermal system[J].Green Chemistry,2014,16(6):3107-3116.[16]QI W Y,HU C W,LI G Y,et al.Catalytic pyrolysis of several kinds of bamboos over zeolite NaY[J].Green Chemistry,2006,8(2):183-190.[17]张雄森.姬松茸高产栽培技术及病虫害防治措施[J].河南农业,2021(2):14-15.[18]钱静亚,张正沛,季蓉蓉,等.3株真菌固态发酵产木质素降解酶的研究[J].江苏农业科学,2017,45(5):277-280.[19]ZHOU H Z,GUO W,XU B,et al.Screening and identification of lignin-degrading bacteria in termite gut and the construction of LiP-expressing recombinant lactococcus lactis[J].Microbial Pathogenesis,2017,112:63-69.[20]KRAMER K J,KANOST M R,HOPKINS T L,et al.Oxidative conjugation of catechols with proteins in insect skeletal systems[J].Tetrahedron,2001,57(2):385-392.[21]CLAUS H.Laccases and their occurrence in prokaryotes[J].Archives of Microbiology,2003,179(3):145-150.[22]PETR B.Fungal laccases-occurrence and properties[J].FEMS Microbiology Reviews,2006,30(2):215-242.[23]PAOLA G,VINCENZA F,CINZIA P,et al.Laccases:A never-ending story[J].Cellular and Molecular Life Sciences,2010,67(3):369-85.[24]徐鑫,张国庆,胡渤洋,等.真菌漆酶及其介体系统:来源、机理与应用[J].生物技术进展,2020,10(1):30-39.[25]NYANHONGO G S,KUDANGA T,PRASETYOE N,et al.Mechanistic insights into laccase-mediated functionalisation of lignocellulose material[J].Biotechnology and Genetic Engineering Reviews,2010,27(1):305-330.[26]TUKAYI K,MARILIZE L R.Laccase applications in biofuels production:Current status and future prospects[J].Applied Microbiology and Biotechnology,2014,98(15):6525-6542.[27]LEVIN L,MELIGNANI E,RAMOS A M.Effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi.Dye decolorization by selected culture filtrates[J].Bioresource Technology,2010,101(12):4554-4563.[28]ERGUN S O,UREK R O.Production of ligninolytic enzymes by solid state fermentation using Pleurotus ostreatus[J].Annals of Agrarian Science,2017,15(2):273-277.[29]GU X Y,FU X,CHEN S L.Hydrothermal liquefaction conversion of lignocelluloses with enhanced fungal pretreatment[J].Industrial Crops & Products,2021,162(1):113268-113281.[30]SHI J,RATNA R.CHINN M,et al.Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production[J].Biomass and Bioenergy,2008,33(1):88-96.[31]WAN C X,YEBO LI Y B.Microbial delignification of corn stover by Ceriporiopsis subvermispora for improving cellulose digestibility[J].Enzyme and Microbial Technology,2010,47(1):31-36.
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备注/Memo
第一作者简介：司徒成（1996-），男，硕士研究生，研究方向为食用菌和植物资源利用。E-mail:2622579262@qq.com.责任作者：余天华（1973-），男，博士，副教授，硕士生导师，现主要从事多相催化及植物资源利用等研究工作。E-mail:Tianhua_yu@163.com.基金项目：贵州省微生物-植物互作及代谢调控科技创新人才团队资助项目（黔科合平台人才[2019]5617号）；贵州师范大学2014年博士科研启动资助项目（600204）。收稿日期：2021-09-06