ZHANG Min,WANG Ziqing,QUAN Xingzhou,et al.Effects of Light Quality on Growth and Photosynthetic Physiology Characteristics of Asarum heterotropoides Fr.schmidt var.mandshuricum (Maxim) kitag[J].Northern Horticulture,2022,(03):107-117.[doi:10.11937/bfyy.20211222]
光质对北细辛生长及光合生理特性的影响
- Title:
- Effects of Light Quality on Growth and Photosynthetic Physiology Characteristics of Asarum heterotropoides Fr.schmidt var.mandshuricum (Maxim) kitag
- Keywords:
- Asarum heterotropoides Fr.schmidt var.mandshuricum (Maxim) kitag; chlorophyll; leaf mass per area; diumal variation of photosynthesis; enzyme activity
- 文献标志码:
- A
- 摘要:
- 以五年生北细辛(Asarum heterotropoides Fr.schmidt var.maudshuricum(Maxim) kitag)为试材,通过覆盖6种不同颜色的滤光膜形成荫棚下光质(Ⅰ~Ⅵ),以生产上常用的50%透光率黑色尼龙网下光质(Ⅶ)为对照,研究不同光质下细辛的田间表现和光合生理特性,以期为北细辛合理调光措施提供参考依据。结果表明:生长在光质Ⅵ下的北细辛植株在5—6月生长状况良好,7月中旬开始地上部逐渐枯死;生长在其它6种光质下的植株,整个生育期生长状况均良好,且增产率为63.30%Ⅳ>60.00%Ⅴ>56.30%Ⅱ>43.30%Ⅰ>35.30%Ⅶ>28.30%Ⅲ。光质Ⅳ和光质Ⅴ下生长的北细辛,叶片光合色素含量(PPC)、全天净光合速率(APn)平均值和比叶重(LMA)均显著高于其它5种光质(P<0.05)。在7种光质下的北细辛,其净光合速率与环境温度均呈显著负相关(P<0.05),蒸腾速率(Tr)与温度均呈显著正相关(P<0.05),净光合速率与胞间二氧化碳浓度(Ci)均呈极显著负相关(P<0.01)。在整个生育期,光质Ⅴ下的北细辛的叶片超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性最大;而光质Ⅳ下的叶片过氧化物酶(POD)活性最大。在7月,光质Ⅵ下生长的北细辛叶片丙二醛(MDA)含量显著高于其它光质。每种光质下,随着生长时间延长,过氧化氢酶活性均呈逐渐降低的趋势,过氧化物酶活性呈升高的趋势,丙二醛含量均先升高后降低。随生长时间的延长,光质Ⅰ~Ⅲ下叶片的超氧化物歧化酶活性先升高后降低,而光质Ⅳ~Ⅶ下叶片的超氧化物歧化酶活性呈逐渐降低趋势。光质Ⅳ和光质Ⅴ有利于北细辛有机物的累积。
- Abstract:
- Taking five-year-old Asarum heterotropoides Fr.schmidt var.mandshuricum (Maxim) kitag as the test materials,six optical filter colorful films and black nylon net having 50% transmittance were conducted to adjust the light quality(Ⅰ-Ⅶ) in the shed.Growing status,leaf mass per area,photosynthetic pigment content,diurnal variation of photosynthesis,protective enzyme activities and malondialdehyde of A.heterotropoides Fr.schmidt var.mandshuricum (Maxim) leaves were detected,in order to study the effect of light quality on growth and photosynthetic physiology characteristics of Asarum heterotropoides Fr.schmidt var.mandshuricum (Maxim) kitag.The results showed that under light qualityⅥ,plants grew well from May to June only and the leaves began to wither gradually in mid July,however,plants grew well in the every growth stages under other light quality conditions and yield increasing rate was 63.30%Ⅳ>60.00%Ⅴ>56.30%Ⅱ>43.30%Ⅰ>35.30%Ⅶ>28.30%Ⅲ.Photosynthetic pigment content,average value of diurnal net photosynthetic rate and leaf mass per area grew under light quality Ⅳ and Ⅴwere significantly higher than those under other light quality conditions (P<0.05).Leaves grew under every light quality condition,there were significant negative correlation between net photosynthetic rate and ambient temperature (P<0.05),significant positive correlation between transpiration rate and ambient temperature (P<0.05),and significant negative correlation between net photosynthetic rate and intercellular carbon dioxide concentration (P<0.01).Activities of superoxide dismutase and catalase of leaves grew under light quality Ⅳwere the highest in all light quality conditions.Activities of peroxidase of leaves under light quality Ⅳwere higher than other light quality conditions.Content of malondialdehyde of leaves under light quality Ⅵwas the highest among all light quality conditions in July.Under each light quality condition,catalase activity decreased gradually,while superoxide dismutase activity and malondialdehyde content reached their maximum in July and August respectively and then decreased,and peroxidase activity increased constantly.The accumulation of organic substances of plantscan be promoted by light quality Ⅳ and Ⅴ by covering optical filter green film and purple film.
参考文献/References:
[1]国家药典委员会.中华人民共和国药典(一部)[S].北京:中国医药科技出版社,2015.[2]蔡少青,李军.常用中药材品种整理和质量研究:北方编[M].北京:北京医科大学出版社,2001.[3]孙洪助,孙文华,刘士辉,等.不同光质对作物形态建成和生长发育的影响[J].安徽农业科学,2015,43(27):17-20.[4]HUCHE-THELIER L,CRESPEL L,GOURRIEREC1 J L et al.Light signaling and plant responses to blue and UV radiations-perspectives for applications in horticulture[J].Environmental and Experimental Botany,2016(121):22-38.[5]郑洁,胡美君,郭延平.光质对植物光合作用的调控及其机理[J].应用生态学报,2008(7):1619-1624.[6]石圆圆.不同LED光质对莴苣生理性状及营养品质的影响[D].郑州:河南农业大学,2018.[7]许莉,刘世琦,齐连东,等.不同光质对叶用莴苣光合作用及叶绿素荧光的影响[J].中国农学通报,2007(1):96-100.[8]林叶春,陈伟,薛原,等.光质对立体浮盘培育烟苗叶片光合特性及根系生长的影响[J].中国农业大学学报,2014,19(1):87-92.[9]何维明,钟章成.攀援植物绞股蓝幼苗对光照强度的形态和生长反应[J].植物生态学报,2000(3):375-378.[10]王志清,郑培和,王英平,等.光照强度对北细辛生长发育及质量的影响[J].中国中药杂志,2011,36(12):1558-1567.[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.[12]WU F Y,YI L T,LI X P,et al.Effect of different light intensity on intensity chlorophyll content and chlorophyll fluorescence in Lithocarpus glaber[J].Journal of Northeast Agricultural University,2012,43(4):88-92.[13]KOCH G W,SILLETT S C,JENNINGS G M,et al.The limits to tree height[J].Nature,2004,428:851-854.[14]李玲.植物生理学模块实验指导[M].北京:科学出版社,2009.[15]高俊凤.植物生理学实验技术[M].西安:世界图书出版社,2000.[16]LI Q,CHIERI K.Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce[J].Environmental and Experimental Botany,2009,67(1):59-64.[17]KOBAYASHI K,AMORE T,LAZARO M.Light-emitting diodes (LEDs) for miniature hydroponic lettucer[J].Optics and Photonics Journal,2013,3(1):74-77.[18]SB A,KREKLING T,APPELGREN M.Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro[J].Plant Cell Tissue Organ Culture,1995,41(2):177-185.[19]储钟稀,童哲,冯丽洁,等.不同光质对黄瓜叶片光合特性的影响[J].植物学报,1999,41(8):867-870.[20]RAMALHO J C,MARQUESN C,SEMEDOJ N,et al.Photosynthetic performance and pigment composition of leaves from two tropical speciesis determined by light quality[J].Plant Biology,2002(4):112-120.[21]王峰,闫家榕,陈雪玉,等.不同光调控植物叶绿素生物合成的研究进展[J],园艺学报,2019,46(5):975-994.[22]YEN N,CHUNG J P.High-brightness LEDs-energy efficient lighting sources and their potential in indoor plant cultivation[J].Renewable & Sustainable Energy Reviews,2009,13(8):2175-2180.[23]洪佳华,马月华,刘明孝,等.光强、光质对人参光合的影响[J].中国农业气象,1995(1):19-22.[24]YOU T,BARNETT S M.Effect of light quality on production of extracellular polysaccharides and growth rate of Porphyridium cruentum[J].Biochemical Engineering Journal,2004,19(3):251-258.[25]张瑞华,徐坤,董灿兴.光质对生姜叶片光合特性的影响[J].中国农业科学,2008,41(11):3722-3727.[26]OHASI-KANEKO K,TAKASE M,KON N,et al.Effect of light quality on growth and vegetable quality in leaf lettuce,spinach and komatsuna[J].Environment Control in Biology,2007(45):189-198.[27]JOHKAN K,SHOJI F.Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativ[J].Environmental and Experimental Botany,2012(75):128-133.[28]谢苗苗,孙翊,殷丽青,等.不同LED光质对矾根组培苗生长和生理特性的影响[J].分子植物育种,2018,16(6):2001-2008.[29]王虹,姜玉萍,师恺,等.光质对黄瓜叶片衰老与抗氧化酶系统的影响[J].中国农业科学,2010,43(3):529-534.[30]方阔,马海琴,王振兴,等.不同光强下北细辛的光合特性及有效成分差异研究[J].中国中药杂志,2019,44(13):2753-2761.
相似文献/References:
[1]顾艾博,董 然,马家艳,等.重金属Cr胁迫下两种玉簪光合特性研究[J].北方园艺,2014,38(13):60.
GU Ai-bo,DONG Ran,MA Jia-yan,et al.Study on Photosynthetic Characteristics in Two Hosta Cultivars Under Heavy Metal Cr Stress[J].Northern Horticulture,2014,38(03):60.
[2]张 芬,张 波,田丽萍,等.盐胁迫对番茄幼苗叶片光合特性及叶绿素和β-胡萝卜素含量的影响[J].北方园艺,2014,38(11):15.
ZHANG Fen,ZHANG Bo,TIAN Li-ping,et al.Effect of Salt Stress on Photosynthetic Characteristic and the Content of Chlorophyll and β-carotene in Tomato Seedling Leaves[J].Northern Horticulture,2014,38(03):15.
[3]陈祥伟,刘世琦,刘 庆,等.不同LED光源对小白菜生长及光合特性的影响[J].北方园艺,2013,37(22):1.
CHEN Xiang-wei,LIU Shi-qi,LIU Qing,et al.Effects of Different Light Qualities on Growth and Photosynthetic Characteristics of Pakchoi[J].Northern Horticulture,2013,37(03):1.
[4]王春丽,楚金萍,阿依夏木·托乎提,等.氮磷钾不同配比对加工番茄育苗质量的影响[J].北方园艺,2014,38(14):14.
WANG Chun-li,CHU Jin-ping,AYIXIAMU Tuohuti,et al.Effect of Different Proportions of Nitrogen,Phosphorus and Potassium on the Quality of Processing Tomato Seedling[J].Northern Horticulture,2014,38(03):14.
[5]王丽媛,周厚高.不同营养组合和施肥量对切花百合光合特性及干物质含量的影响[J].北方园艺,2014,38(04):58.
WANG Li-yuan,ZHOU Hou-gao.Effect of Nutrient Combinations and Fertilizing Amount on the Photosynthetic Characteristics and Dry Matter of Cut-flower Lily[J].Northern Horticulture,2014,38(03):58.
[6]张振宇,王鹏,王淑安,等.‘金薇’叶片呈色的结构和生理基础研究[J].北方园艺,2014,38(04):68.
ZHANG Zhen-yu,WANG Peng,WANG Shu-an,et al.Study on Structure and Physiological Basis of Leaf Colouring in Lagerstroemia indica L.‘Jinwei’[J].Northern Horticulture,2014,38(03):68.
[7]郑宝刚,叶露幻,宋涛,等.银杏新梢叶片光合与荧光特性研究[J].北方园艺,2013,37(13):65.
ZHENG Bao-gang,YE Lu-huan,SONG Tao,et al.Study on Photosynthesis and Fluorescence Characteristics of Ginkgo biloba L.Shoots Leaves[J].Northern Horticulture,2013,37(03):65.
[8]王少平,黄超.切花菊‘黄中黄’对干旱胁迫的生理响应[J].北方园艺,2013,37(15):64.
WANG Shao-ping,HUANG Chao.Physiological Response of Cut Chrysanthemum ‘Huangzhonghuang’ to Drought Stress[J].Northern Horticulture,2013,37(03):64.
[9]许华,陈甜甜,朱敏杰,等.三裂叶蟛蜞菊入侵对三种杂草若干生理特性的影响[J].北方园艺,2014,38(07):72.
XU Hua,CHEN Tian-tian,ZHU Min-jie,et al.Effect of Wedelia trilobata L.Invasion on Several Physiological Characteristics of Three Weeds[J].Northern Horticulture,2014,38(03):72.
[10]邱清华,邓绍云.盐胁迫对七个品种紫菜薹种子萌发及幼苗生长的影响[J].北方园艺,2013,37(18):27.
QIU Qing-hua,DENG Shao-yun.Influence of Salt Stress on Seeds Germination and Seedling Growth of Brassica campestris [J].Northern Horticulture,2013,37(03):27.
备注/Memo
第一作者简介:张敏(1995-),女,硕士研究生,研究方向为药用植物学。E-mail:419558536@qq.com.责任作者:王志清(1976-),女,博士,副教授,硕士生导师,现主要从事中药资源与开发等研究工作。E-mail:wangzhiqing96@sohu.com.基金项目:吉林省科技厅技术攻关资助项目(20190304017YY);国家重点研发计划资助项目(2019YFC1710700);吉林省中医药管理局资助项目(L202009)。收稿日期:2021-03-22