ZHOU Wuxian,LI Darong,GONG Siyu,et al.Effects of Exogenous Zinc on Seed Germination and Seedling Growth of Atractylodes macrocephala[J].Northern Horticulture,2022,(03):98-106.[doi:10.11937/bfyy.20213406]
外源锌对白术种子萌发及幼苗生长的影响
- Title:
- Effects of Exogenous Zinc on Seed Germination and Seedling Growth of Atractylodes macrocephala
- 文献标志码:
- A
- 摘要:
- 以白术种子为试材，采用盆栽试验法，研究外源锌对白术种子萌发和幼苗生长的影响，以期为白术科学施锌和种苗高效繁育提供参考依据。结果表明：外源锌对白术幼苗生长具有低浓度（≤100 mg·L-1）促进，高浓度（200 mg·L-1）抑制的效果。其中100 mg·L-1的锌处理对白术种子萌发和幼苗生长的促进效果最好，与对照相比，提高了白术幼苗的根长、株高、叶片数和鲜质量，增幅分别达到68.3%、29.1%、21.2%和20.0%；幼苗叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量也显著高于对照，增幅分别为16.7%、26.2%、19.4%和5.9%。此外，100 mg·L-1锌处理显著提高白术幼苗叶片可溶性蛋白质(SP）含量，降低过氧化氢酶（CAT）活性和超氧化物歧化酶（SOD）活性，减少叶片可溶性糖含量（SS）和丙二醛（MDA）含量。200 mg·L-1锌处理对白术幼苗的生长具有显著的抑制作用，根长、株高、鲜质量、干质量及叶绿素含量均显著低于对照。综上所述，外源锌对白术幼苗生长具有典型的剂量效应，施用100 mg·L-1的锌肥对白术幼苗生长的促进效果最佳。
- Abstract:
- Taking Atractylodes macrocephala as test material,the effects of exogenous zinc (Zn) on seed germination and seedling growth of Atractylodes macrocephala were explored through a pot experiment,in order to provide reference for rational zinc application and efficient breeding of A.macrocephala.The results showed that the growth of A.macrocephala seedlings was promoted by low Zn levels (100 mg·L-1) and inhibited by high levels (200 mg·L-1).100 mg·L-1 Zn treatment had the best effect on the seed germination and seedling growth of A.macrocephala.Compared with the control,100 mg·L-1 Zn treatment significantly increased the root length,plant height,leaf number and fresh weight of A.macrocephala seedlings by 68.3%,29.1%,21.2% and 20.0%,respectively.The content of chlorophyll a,chlorophyll b,total chlorophyll and carotenoid in A.macrocephala seedling leaves under 100 mg·L-1 Zn treatment were also significantly increased by 16.7%,26.2%,19.4% and 5.9%,respectively,compared with the control.Moreover,the content of soluble protein (SP) in A.macrocephala seedling leaves was significantly increased while the soluble sugar (SS) and malondialdehyde (MDA) were decreased under the 100 mg·L-1 Zn treatment,compared with the control.Meanwhile,the activities of catalase (CAT) and superoxide dismutase (SOD) in A.macrocephala seedling leaves were also significantly decreased under the 100 mg·L-1 Zn treatment,compared with the control.However,the root length,plant height,fresh weight,dry weight and chlorophyll content of A.macrocephala seedlings under 200 mg·L-1 Zn treatment were significantly lower than those of the control.In conclusion,Zn had a typical dose effect on the growth of A.macrocephala seedlings,and 100 mg·L-1 Zn should be recommended.
参考文献/References:
[1]李娟,陈杰忠,黄永敬,等.Zn营养在果树生理代谢中的作用研究进展[J].果树学报,2011,28(4):668-673.[2]AULD D S.Zinc coordination sphere in biochemical zinc sites[J].BioMetals,2001(14):3-4.[3]尚杨娟,谭鹏鹏,范平桦,等.薄壳山核桃叶面喷锌效果的评价[J].浙江农林大学学报,2020,37(6):1071-1079.[4]赵晓祥,冯璐,王宇晖.锌、镉单一及复合胁迫下番茄幼苗生理响应及联合毒性的研究[J].安全与环境学报,2020,20(3):1176-1184.[5]李宇航,杜天庆,龚芮,等.叶面喷锌对糯玉米生理特性、籽粒锌含量及产量的影响[J].河南农业科学,2020,49(10):26-32.[6]MICHAEL P I，KRISHNASWAMY M.The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings[J].Environ and Exp Bot,2011,74:171-177.[7]龚红梅,李卫国.锌对植物的毒害及机理研究进展[J].安徽农业科学,2009,37(29):14009-14015.[8]杨洁,方成武.中药白术的质量研究现状与展望[J].中国当代医药,2010,17(1):5-7.[9]张晓娟,左冬冬.白术化学成分及药理作用研究新进展[J].中医药信息,2018,35(6):101-106.[10]肖仲久,李小霞.白术叶枯病病原菌鉴定及其生物学特性研究[J].热带作物学报,2013,34(5):973-977.[11]周晓龙,厉金荣,米超斌,等.白术锌肥试验初报[J].中药材,1995(12):599-601.[12]WALWORTH J L.Fall-applied foliar zinc for pecans[J].HortScience，2006,41(1):275-276.[13]PRASAD R,SHIVAY Y S,KUMAR D.Agronomic biofortification of cereal grains with iron and zinc[J].Advan in Agron,2014,125:55-91.[14]沈宇峰,王志安,俞旭平,等.白术种子生活力测定方法及其与发芽率的相关性研究[J].中国中药杂志,2008(3):248-250.[15]周武先,段媛媛,卢超,等.高效提取3种不同类型植物叶片色素的方法[J].西北农业学报,2019,28(1):97-104.[16]施海涛.植物逆境生理学实验指导[M].北京:科学出版社,2016.[17]周武先,何银生,朱盈徽,等.生石灰和钙镁磷肥对酸化川党参土壤的改良效果[J].应用生态学报,2019,30(9):3224-3232.[18]赵玉红,拉巴曲吉,罗布,等.铜、镉、铅、锌对4种豆科植物种子萌发的影响[J].种子,2017,36(1):22-28.[19]蔡鑫鑫,杨克军,王玉凤,等.锌肥对寒地春玉米幼苗生长发育及光合特性的影响[J].中国西部科技,2014,13(1):51-53.[20]余国源,王佳星,黄琛斐,等.土壤锌胁迫对伞房决明生长及生理的影响[J].福建农林大学学报(自然科学版),2019,48(4):517-523.[21]刘玲丽,颉建明,张帆,等.锌对青花菜种子萌发的影响[J].甘肃农业大学学报,2014,49(2):102-105,111.[22]宣毓龙,庞庆阳,王开勇,等.锌胁迫对黑小麦种子萌发、幼苗生长和酶活性的影响[J].新疆农业科学,2016,53(5):907-914.[23]冷芬,杨在君,吴一超,等.土壤pH值对何首乌生理及其光合特性和有效成分含量的影响[J].西北植物学报,2020,40(9):1566-1573.[24]孙卓,李亚迪,纪建伟.番茄幼苗叶绿素荧光参数对锌胁迫的响应[J].北方园艺,2016(24):41-44.[25]李文一,徐卫红,胡小凤,等.Zn胁迫对黑麦草幼苗生长、生理生化及Zn吸收的影响[J].农业工程学报,2007(5):190-194.[26]朱红霞,杨小勇,葛才林,等.重金属对水稻过氧化物酶同功酶的影响[J].核农学报,2004(3):233-236,169.[27]刘彬,曹尚杰,王营,等.过表达细叶百合LpNAC6基因增强烟草的耐盐性[J].北京林业大学学报,2020,42(4):69-79.[28]胡文俐,李培旺,李昌珠,等.铅锌胁迫对山苍子幼苗生理生化特性的影响[J].中南林业科技大学学报,2019,39(9):109-114.[29]段媛媛,宋丽娟,牛素旗,等.不同林龄刺槐叶功能性状差异及其与土壤养分的关系[J].应用生态学报,2017,28(1):28-36.
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备注/Memo
第一作者简介：周武先（1991-），男，硕士，助理研究员，现主要从事药用植物资源的评价与利用等研究工作。E-mail:zhou_wx222@163.com.责任作者：王华（1979-），女，硕士，助理研究员，现主要从事中药材育种与栽培等研究工作。E-mail:285769813@qq.com.基金项目：湖北省重点研发计划资助项目（2020BCA059）；湖北省农业科技创新中心重大科技研发资助项目（2020-620-000-002-04）；湖北省技术创新专项资助项目（2019AKB092）；恩施州科技计划资助项目（D20200017,D20200020）。收稿日期：2021-08-19