LIU Meng,HE Hongju,HUANG Xiaoxin,et al.Effects of Exogenous Substance Na2SeO3 Treatment on Nutritional Quality and Glucosinolate Content of Broccoli[J].Northern Horticulture,2021,(21):1-7.[doi:10.11937/bfyy.20210686]
外源物质亚硒酸钠处理对西兰花营养品质和硫苷含量的影响
- Title:
- Effects of Exogenous Substance Na2SeO3 Treatment on Nutritional Quality and Glucosinolate Content of Broccoli
- Keywords:
- broccoli; Na2SeO3; nutritional quality; glucosinolates
- 文献标志码:
- A
- 摘要:
- 以西兰花“耐寒优秀”品种为试材,采用不同浓度(0、2.5、5.0、10.0、25.0 mg·L-1)的外源物质Na2SeO3处理西兰花,通过测定西兰花中的维生素C、可溶性糖、可溶性蛋白质、粗纤维、多酚、总黄酮和硫苷含量,研究了外源物质Na2SeO3对西兰花营养品质和硫苷含量的影响,以期为强化西兰花营养与活性成分提供参考依据。结果表明:在Na2SeO3不同浓度处理下,西兰花中的可溶性糖含量无显著差异;Na2SeO3处理浓度为5.0 mg·L-1时,维生素C、可溶性蛋白质、干样粗纤维含量最高,较对照分别提高5.31%、17.99%和6.02%。当Na2SeO3处理浓度为10.0 mg·L-1时多酚含量最高,而浓度为5.0 mg·L-1时其次,二者之间差异不显著,较对照分别提高20.18%和4.61%;Na2SeO3处理浓度为5.0 mg·L-1促进了西兰花中除ERU和4OH以外的脂肪族硫苷、吲哚族硫苷和总硫苷含量增加,且在所有处理中这些硫苷含量均为最高。综上,以Na2SeO3 5.0 mg·L-1处理对西兰花营养品质和硫苷含量产生的效果最优。
- Abstract:
- ‘Naihanyouxiu’ broccoli variety used as the test material,different concentrations (0 mg·L-1,2.5 mg·L-1,5.0 mg·L-1,10.0 mg·L-1,25.0 mg·L-1) of exogenous substance Na2SeO3 were used to treat broccoli.The effects of different concentrations of exogenous substance Na2SeO3 on the nutritional quality and glucosinolate content of broccoli were studied by determining the contents of vitamin C,soluble sugar,soluble protein,crude fiber,polyphenols,total flavonoids and glucosinolates,in order to provide technical reference for strengthening the nutrition and active ingredient of broccoli.The results showed that under the treatment of different concentration of Na2SeO3,there was no significant difference of soluble sugar in broccoli;when the concentration of Na2SeO3 was 5.0 mg·L-1,the contents of vitamin C and soluble protein and dry crude fiber were the highest,which were increased by 5.31%,17.99% and 6.02%,compared with CK,respectively.When the concentration of Na2SeO3 was 10.0 mg·L-1,the polyphenol content was the hightest,when the concentration was 5.0 mg·L-1,it was the second content,there was no significant difference between them,which increased by 20.18% and 4.61%,compared with CK,respectively.When the concentration of Na2SeO3 was 5.0 mg·L-1,the contents of aliphatic glucosinolates (not included ERU and 4OH),indole glucosinolates and total glucosinolates in broccoli were increased,and the content of these glucosinolates were the highest in all treatments.In conclusion,different concentrations of exogenous substance Na2SeO3 on the nutritional quality and glucosinolate content of broccoli produced a greater impact,which the concentration of 5.0 mg·L-1 Na2SeO3 had the most efficient.
参考文献/References:
[1]李钧敏,金则新,柯喜丹.西兰花品种的随机扩增多态DNA分析[J].江苏农业科学,2006(4):66-70.[2]李宁,阎瑞香,张娜.LED复合光处理对西兰花低温保鲜效果的影响[J].华北农学报,2015,30(1):188-193.[3]王慧倩,郑聪,王华东,等.乙醇熏蒸处理对鲜切西兰花活性成分和抗氧化活性的影响[J].食品科学,2014,35(16):250-254.[4]龚春燕,苏娜娜,陈沁,等.不同光质对西兰花芽苗菜营养品质及抗氧化性的影响[J].食品工业科技,2018,39(23):42-49.[5]HERR I,BUCHLER M W.Distary constituents of broccoli and other cruciferous vegetables:Implications for prevention and therapy of cancer[J].Cancer Treatment Reviews,2010(36):377-383.[6]何洪巨,陈杭,SCHNITZLER W H.芸薹属蔬菜中硫代葡萄糖苷鉴定与含量分析[J].中国农业科学,2002,35(2):192-197.[7]CLARKE D B.Glucosinolates,structures and analysis in food[J].Analytical Methods,2020(2):310-325.[5]李向果.西兰花叶中硫代葡萄糖苷和原花青素的生物学效价评定[D].兰州:甘肃农业大学,2013.[6]张华,曾桥.原花青素功能及应用进展[J].安徽农业科学,2011,39(9):5349-5350.[7]赵晓嫚.青花菜小孢子胚胎发生及品质影响因素研究[D].南京:南京农业大学,2013.[8]李爱莲.青花菜营养品质的分析及杂种优势的遗传研究[D].泰安:山东农业大学,2015.[9]孙崇庆,马晓春,高艳明,等.硒肥对植物工厂水培生菜生长及品质的影响[J].中国瓜菜,2020,33(6):24-29.[10]李磊,尹显慧,龙友华,等.叶面喷施硒对辣椒果实品质及微量元素的影响[J].北方园艺,2019(16):1-6.[11]王晋民,赵之重,沈增基.叶面施硒对青花菜含硒量及产量与品质的影响[J].西北农林科技大学学报(自然科学版),2006(3):127-130.[12]成凯.硒硫互作对青花菜幼苗生理及光合特性的影响[D].成都:四川农业大学,2016.[13]柏夏琼,陈介南,张林,等.发芽条件对西兰花芽苗菜总黄酮富集的影响[J].食品研究与开发,2020,41(1):105-110.[14]王丽丽.镉、硒处理对碎米荠和芥蓝生长及生理品质的影响[D].杭州:浙江农林大学,2019.[15]饶帅琦,陈晓琪,杨静,等.环境因子对硫代葡萄糖苷影响的研究进展[J].植物生理学报,2020,56(9):1765-1772.[16]ROBBINS R J,KECK A S,BANUELOS G,et al.Cultivation conditions and selenium fertilization alter the phenolic profile,glucosinolate,and sulforaphane content of broccoli[J].Journal of Medicinal Food,2005(8):204-214.[17]白宝璋,靳占忠,李存东.植物生理学(下:实验教程)[M].北京:中国农业科技出版社,2001.[18]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.[19]张建.不同无土栽培基质对高羊茅草皮生产的影响[D].成都:四川农业大学,2015.[20]田璐.NaCl和CaCl2处理对西兰花芽苗菜营养与功能品质的影响[D].南京:南京农业大学,2017.[21]赵登奇,孙亚天,黄建颖,等.西兰花叶中生物活性成分的测定[J].核农学报,2020,34(6):1266-1271.[22]刘倩男,黄伟,丁云花,等.青花菜中硫代葡萄糖苷RAA和GBC的近红外光谱快速测定[J].中国农业科学,2020,53(21):4497-4506.[23]冯晓汀,刘洪丽,吴秀,等.乙醇对鲜切西兰花品质及其生理、生化代谢的影响[J].食品科学技术学报,2015,33(6):18-23.[24]张怡,关文强,张娜,等.温度对西兰花抗氧化活性及其品质指标影响[J].食品研究与开发,2011,32(8):156-161.[25]姚小桐,张润芝,许宁,等.不同品种芸薹属蔬菜中硫代葡萄糖苷含量的比较分析[J].北方园艺,2018(21):30-36.[26]丁云花,何洪巨,宋曙辉,等.不同西兰花品种中硫代葡萄糖苷的组分与含量分析[J].长江蔬菜,2015(20):70-73,74.[27]许春雷,钱仲仓,陈能品.西兰花中硫代葡萄糖苷的研究进展[J].现代农业科技,2014(7):295,299.[28]姚丹燕.茉莉酸甲酯和硒对青花菜中萝卜硫素含量的影响研究[D].长沙:湖南农业大学,2015.[29]田明.硒处理对西兰花中硫代葡萄糖苷合成及代谢影响的研究[D].武汉:华中农业大学,2017.[30]韩亚文,韩莹琰,郭婉香,等.土壤施硒对番茄果实品质的影响[J].中国农学通报,2014,30(13):220-224.[31]丁一.硒硫互作对小白菜植株生长及抗氧化品质的影响[D].杭州:浙江农林大学,2014.]
相似文献/References:
[1]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(21):158.
[2]李晓旭,李家政.西兰花采后生理及保鲜技术研究进展[J].北方园艺,2013,37(14):196.
LI Xiao-xu,LI Jia-zheng.Research Process on Postharvest Physiology and Preservation Technology of Broccoli[J].Northern Horticulture,2013,37(21):196.
[3]汪恩国,刘伟明.台州西兰花斜纹夜蛾种群数量消长规律研究[J].北方园艺,2013,37(15):137.
WANG En-guo,LIU Wei-ming.Study on Spodoptera litura(Fabricins) Population Dynamics Regularity on Broccoli in Taizhou Area[J].Northern Horticulture,2013,37(21):137.
[4]刘然然,寇莉萍,阎瑞香.发光二极管绿光照射对精品蔬菜货架期品质的影响[J].北方园艺,2013,37(08):5.
LIU Ran-ran,KOU Li-ping,YAN Rui-xiang.Effects of LED Green Light Irradiation on Quality of Vegetables in Shelf Life[J].Northern Horticulture,2013,37(21):5.
[5]刘伟明,汪恩国.西兰花高产高效栽培中主要栽培因子与其生育期和产量关系的数学模型研究[J].北方园艺,2012,36(08):48.
LIU Wei-ming,WANG En-guo.Research on the Mathematical Models Between the Main Cultivation Factor and Reproductive Stage and Yield of Broccoli[J].Northern Horticulture,2012,36(21):48.
[6]翁 梁,温 鲁.硒和钙对蛹虫草活性物质含量的影响[J].北方园艺,2012,36(22):159.
WENG Liang,WEN Lu.Effects of Selenium and Calcium on the Content of Cordyceps militaris Active Substances[J].Northern Horticulture,2012,36(21):159.
[7]王文举,王振平.亚硒酸钠对“红地球”葡萄耐热性及果实品质的影响[J].北方园艺,2012,36(24):47.
WANG Wen-ju,WANG Zhen-ping.The Effect of Sodium Selenite on Heat-resistance and Berry Quality of Red Globe Grape[J].Northern Horticulture,2012,36(21):47.
[8]薛美琼.西兰花漂浮育苗与湿润育苗对比研究[J].北方园艺,2014,38(16):34.
XUE Mei-qiong.Comparative Study on Broccoli Floating Seedlings and Moisture Seedlings[J].Northern Horticulture,2014,38(21):34.
[9]刘会玲,梁硕,王艳群,等.西兰花优化施肥及适宜微生物肥料筛选[J].北方园艺,2015,39(08):162.[doi:10.11937/bfyy.201508043]
LIU Hui-ling,LIANG Shuo,WANG Yan-qun,et al.The Optimized Fertilization and Screening of Suitable Microbial Fertilizer on Broccoli[J].Northern Horticulture,2015,39(21):162.[doi:10.11937/bfyy.201508043]
[10]吕凤艳,郭衍银,王兆全.西兰花气调保鲜过程中O2缓解CO2伤害的研究[J].北方园艺,2016,40(13):132.[doi:10.11937/bfyy.201613035]
LYU Fengyan,GUO Yanyin,WANG Zhaoquan.Study on the Alleviation of O2 to CO2 Injury During Broccoli Storage Under Controlled Atmospheres[J].Northern Horticulture,2016,40(21):132.[doi:10.11937/bfyy.201613035]
备注/Memo
第一作者简介:刘萌(1995-),女,硕士研究生,研究方向为蔬菜营养与品质。E-mail:1430171857@qq.com.责任作者:黄伟(1970-),男,硕士,教授,现主要从事蔬菜生理生态和营养等研究工作。E-mail:nlkjxyyy@163.com.基金项目:北京市农林科学院创新能力建设专项资助项目(KJCX20200213);河北省专业学位研究生教学案例建设资助项目(KCJSZ2020101);国家大宗蔬菜现代农业产业技术体系建设资助项目(CARS-23-G-08);河北省现代农业产业技术体系蔬菜产业创新团队建设资助项目(HBCT2018030212)。收稿日期:2021-02-18