[1]董瑞瑞,董诚明,申鹏龙,等.基于EST-SSR标记的怀地黄遗传特性[J].北方园艺,2021,(15):119-125.[doi:10.11937/bfyy.20204900]
　DONG Ruirui,DONG Chengming,SHEN Penglong,et al.Genetic Characteristics of Rehmannia glutinosa Based on EST-SSR Marker[J].Northern Horticulture,2021,(15):119-125.[doi:10.11937/bfyy.20204900]
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基于EST-SSR标记的怀地黄遗传特性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 期数: 2021年15 页码: 119-125 栏目: 出版日期: 2021-08-15
Title:
Genetic Characteristics of Rehmannia glutinosa Based on EST-SSR Marker
作者:
董瑞瑞1; 董诚明1; 2; 3; 申鹏龙1; 余孟娟1; 王鑫源1; 张岩岩1
（1.河南中医药大学 药学院，河南 郑州 450046;2.河南中医药大学 河南省道地药材生态种植工程技术研究中心，河南 郑州 450046;3.河南中医药大学 呼吸疾病中医药防治省部共建协同创新中心，河南 郑州 450046）
Author(s):
DONG Ruirui1; DONG Chengming1; 2; 3; SHEN Penglong1; YU Mengjuan1; WANG Xinyuan1; ZHANG Yanyan1
(1.School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou,Henan 450046;2.Dao-di Herbs Ecological Cultivation Engineering Research Center of Henan Province,Henan University of Chinese Medicine,Zhengzhou,Henan 450046;3.Provincial and Ministry Co-construction of Collaborative Innovation Center About Prevention and Treatment of Respiratory Diseases in Traditional Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou,Henan 450046)
关键词:
地黄; EST-SSR; 品种; 遗传特性
Keywords:
Rehmannia glutinosa; EST-SSR; variety; genetic characteristics
DOI:
10.11937/bfyy.20204900
文献标志码:
A
摘要:
以怀地黄新品种“怀中1号”和道地产区5个主流品种“金九”“怀丰”“北京3号”“沁怀”“85-5”共60个样本为试材，采用EST-SSR分子标记方法，研究不同怀地黄品种间遗传特性，以期为分子标记辅助育种提供参考依据。结果表明：从68对引物中筛选出9对具有多态性的引物，每对引物R检测到的等位基因数8~25个，平均15个。共检测到137个等位基因（分子量变异范围为100~500 bp），多态位点百分率97.08%。SSR多态信息量（PIC）分布范围为0.850 1~0.926 4，平均为0.900 7。有效等位基因1.153 1~1.201 2，Nei′s 基因多样性为0.085 7~0.111 3，Shannon指数为0.124 6~0.160 5,不同品种的遗传距离范围0.148 6~0.590 9。聚类分析结果显示，遗传相似系数为0.59时，6个品种分为3类，在遗传相似系数0.79处，每个品种的10个样本单独聚为一类。表明SSR分子标记可以用于地黄不同品种之间的亲缘关系鉴定以及品种纯度的验证。
Abstract:
Taking the new Rehmannia glutinosa variety ‘Huaizhong No.1’ and the five mainstream varieties of genuine producing area ‘Jinjiu’‘Huaifeng’‘Beijing No.3’‘Qinhuai’‘85-5’,a total of 60 samples were taken as test materials,using EST-SSR molecular marker method,to study the genetic characteristics of different varieties of Rehmannia glutinosa,in order to provide reference for molecular marker assisted breeding.The results showed that 9 pairs of primers with polymorphism were selected from 68 pairs of primers.The number of alleles detected by each pair of primers were 8-25,with an average of 15 alleles.A total of 137 alleles (the range of molecular weight variation is 100-500 bp) were detected,and the percentage of polymorphic sitesis 97.08%.The distribution range of SSR polymorphic information content (PIC) was 0.850 1-0.926 4,with an average of 0.900 7.Effective alleles were 1.153 1-1.201 2,Nei′s gene diversity was 0.085 7-0.111 3,Shannon index was 0.124 6-0.160 5,and the genetic distance of different breeds ranges from 0.148 6 to 0.590 9.The cluster analysis results showed that when the coefficient of genetic similarity was 0.59,the 6 varieties were divided into 3 groups.At the genetic similarity coefficient of 0.79,10 samples of each variety were clustered into one group.It shows that SSR molecular markers can be used to identify the genetic relationship between different varieties of Rehmannia glutinosa and verify the purity of the varieties.

参考文献/References:

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备注/Memo

第一作者简介：董瑞瑞（1996-），女，硕士研究生，研究方向为中药材规范化种植技术。E-mail:1206143991@qq.com.责任作者：董诚明（1963-），男，本科，教授，现主要从事中药材规范化种植技术等研究工作。E-mail:dcm663@sina.com.基金项目：国家重点研发计划资助项目（171100310500）；河南省重大科技专项资助项目（2017YFC1702800)。收稿日期：2020-11-19

更新日期/Last Update: 2021-11-23