JIANG Hui,LIN Chenyi,GAO Pan,et al.Effects of Five Inorganic Salts on the Mycelial Growth of Stropharia rugoso-annulata[J].Northern Horticulture,2020,44(14):128-135.[doi:10.11937/bfyy.20194950]
五种无机盐对大球盖菇菌丝生长的影响
- Title:
- Effects of Five Inorganic Salts on the Mycelial Growth of Stropharia rugoso-annulata
- Keywords:
- Stropharia rugoso-annulata; inorganic salt; culture medium; orthogonal optimization; mycelial growth evaluation value
- 文献标志码:
- A
- 摘要:
- 以大球盖菇母种为试材，采用平板培养法，在完全培养基中分别添加不同浓度的单盐，筛选出3种无机盐，采用L9（33）正交实验设计，以菌丝生长能力评价值为判断指标，分析不同浓度的单盐及其组合对大球盖菇菌丝生长的作用。结果表明：供试的5种无机盐对大球盖菇菌丝生长均有促进作用，其中1.000 g?L-1 CaSO4、1.500 g?L-1 CaCl2、1.500 g?L-1 K2SO4、3.500 g?L-1 KH2PO4和0.125 g?L-1 MgSO4能显著促进菌丝生长。3种无机盐组合对大球盖菇菌丝生长影响主次顺序依次为MgSO4>KH2PO4>K2SO4，最优组合为0.250 g?L-1 K2SO4+0.500 g?L-1 MgSO4+3.500 g?L-1 KH2PO4。Ca2+、K+和Mg2+对菌丝生长存在显著影响，其中K+作用效果最明显；钙源和钾源不同对大球盖菇菌丝生长的促进效果不同；3种无机盐之间存在一定浓度的配比，其中MgSO4的影响最大，其次为KH2PO4。生产中营养配方应注重补充K+和Mg+。以期筛选出适合大球盖菇菌丝生长的最佳无机盐种类及其组合，提高菌丝生物量、延迟衰老，达到林下栽培大球盖菇生产实践以及防止菌种退化、老化的目的。
- Abstract:
- Taking the parent species of Stropharia rugoso-annulata as the test material,three inorganic salts were screened out by adding different concentrations of single salt into the complete medium in the plate culture method.Using L9 (33) orthogonal test design and the evaluation value of mycelial growth ability as the judgment index,the effect of single salt and its combination of different concentrations on the mycelial growth of Stropharia rugoso-annulata was analyzed.The results showed that five kinds of inorganic salts could promote the mycelial growth of Stropharia rugoso-annulata.1.000 g?L-1 CaSO4,1.500 g?L-1 CaCl2,1.500 g?L-1 K2SO4,3.500 g?L-1 KH2PO4 and 0.125 g?L-1 MgSO4 could significantly promote the mycelial growth.Effects of three inorganic salts on the mycelial growth showed the impact order was MgSO4>KH2PO4>K2SO4,and the best combination for the mycelia growth of Stropharia rugoso-annulata was 0.250 g?L-1 K2SO4+0.005 g?L-1 MgSO4+3.500 g?L-1 KH2PO4.Ca2+,K+ and Mg2+had a significant effect on the growth of mycelia,of which K+ had the most obvious effect;different Ca and K sources had different effects on the growth of Stropharia rugoso-annulata;there was a concentration ratio among the three inorganic salts,MgSO4 had the greatest effect,followed by KH2PO4.K+ and Mg+ should be supplemented in nutritional formula during production.In order to screen out the best inorganic salt species and their combinations were selected for the mycelia growth of Stropharia rugoso-annulata,improve the biomass of mycelia,delay the senescence,and achieve the purpose of cultivation of Stropharia rugoso-annulata in the forest and prevent the degradation and senescence of the strains.
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备注/Memo
第一作者简介：姜慧（1994-），女，硕士，助教，现主要从事食用菌栽培与育种等研究工作。E-mail：1127373033qq.com.责任作者：林辰壹（1965-）,女，博士，教授，现主要从事食用菌种质资源等研究工作。E-mail：linchenyi65@sina.com.基金项目：新疆维吾尔自治区园艺重点学科基金资助项目（2016-10758-3）；大学生创新资助项目（2020-407）。收稿日期：2020-03-18