ZHANG Yan-zhen,ZHOU Hui-ming,WEI Sheng-long,et al.Optimization Test of Four Inorganic Salts on the Mycelia Growth of Lyophyllum decastes[J].Northern Horticulture,2014,38(05):135-138.
四种无机盐对荷叶离褶伞菌丝生长的优化试验
- Title:
- Optimization Test of Four Inorganic Salts on the Mycelia Growth of Lyophyllum decastes
- 文章编号:
- 1001-0009(2014)05-0135-04
- 分类号:
- S 646.19
- 文献标志码:
- A
- 摘要:
- 以荷叶离褶伞菌株为试材，采用L9（34）正交实验设计，在PDA基础培养基上测定了4种无机盐及其正交组合对其菌丝生长的影响，以期为其优质栽培提供理论依据。结果表明：荷叶离褶伞菌丝萌发与生长的最适组合为ZnSO4 2.0 g/L、MgSO4 4.0 g/L、KH2PO4 4.0 g/L、FeSO4 1.5 g/L；高浓度的ZnSO4、MgSO4、KH2PO4以及FeSO4对菌丝生长有抑制作用，其中MgSO4浓度≥10.0 g/L时，菌丝停止生长；4种无机盐协同作用对荷叶离褶伞菌丝生长影响的大小依次为FeSO4>MgSO4>KH2PO4>ZnSO4；各组合之间的菌丝生长速度差异极显著，不同组合间有明显的交互作用，最佳组合为2.0 g/L ZnSO4+6.0 g/L MgSO4+6.0 g/L KH2PO4+1.0 g/L FeSO4。
- Abstract:
- Taking Lyophyllum decastes as material,effects of four inorganic salts and their orthogonal combination on the mycelial growth of Lyophyllum decastes were investigated by L9(34) orthogonal design on the PDA culture medium in this study，it could provide the theoretical basis of its high quality cultivation.The results showed that the best concentration on optical mycelia germination and growth of Lyophyllum decastes was ZnSO4 2.0 g/L，MgSO4 4.0 g/L，KH2PO4 4.0 g/L，FeSO4 1.5 g/L.ZnSO4，MgSO4，KH2PO4 and FeSO4 at high concentration could inhibit the mycelia growth，and mycelium could stop growth at a concentration of MgSO4≥10 g/L.The cooperative effects of four inorganic salts on the mycelial growth showed the impact order was FeSO4>MgSO4>KH2PO4>ZnSO4.Mycelial growth rate revealed that each combination had a highly significant difference and significant interaction.The best combination for the mycelia growth of?Lyophyllum decastes was 2.0 g/L ZnSO4+6.0 g/L MgSO4+6.0 g/L KH2PO4+1.0 g/L FeSO4.
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备注/Memo
第一作者简介：张焱珍（1986-）,女，硕士，助教，研究方向为食用菌资源驯化与遗传。E-mail：421154119@qq.com.