ZHAO Yuting,XING Jinyue,FANG Xue,et al.Optimization of Extraction Technology of Resveratrol From Veratrum nigrum Linn and Its Inhibitory Effect on Alternaria panax[J].Northern Horticulture,2020,44(17):110-117.[doi:10.11937/bfyy.20194188]
藜芦中白藜芦醇提取工艺的优化及对人参黑斑病菌的抑制效果
- Title:
- Optimization of Extraction Technology of Resveratrol From Veratrum nigrum Linn and Its Inhibitory Effect on Alternaria panax
- Keywords:
- high performance liquid chromatography; response surface analysis; resveratrol; Alternaria panax
- 文献标志码:
- A
- 摘要:
- 以藜芦为试材，采用回流、超声波等方法提取藜芦粗粉，并采用高效液相色谱法测定了各个单因素试验中有效成分白藜芦醇含量，根据含量利用响应面分析法寻找最佳提取工艺，进一步采用菌丝生长速率法测定了对人参黑斑病菌的抑菌效果，以期探讨植物藜芦中白藜芦醇提取工艺的优化，并为植物提取物防治人参黑斑病提供参考依据。结果表明：藜芦提取物对人参黑斑病菌的EC50值由小到大依次为甲醇提取物＜乙醇提取物＜丙酮提取物，藜芦甲醇提取物对人参黑斑病的防治效果优于其它提取物，EC50值为32.04 μg?mL-1；藜芦甲醇提取物的有效成分白藜芦醇最佳提取条件为提取温度59 ℃，提取时间1.5 h，甲醇体积分数50%，最佳提取量9.33 μg?g-1。
- Abstract:
- Crude powder of Veratrum nigrum Linn was extracted by refluxing,ultrasonic and other methods,and the content of resveratrol in each single factor test was determined by high performance liquid chromatography.According to the content,response surface analysis was used to find the best extraction process.The antifungal effect of resveratrol on Alternaria panax was further determined by mycelial growth rate method,in order to explore the optimize extraction process of resveratrol from plant V.nigrum Linn,and to provide theoretical basis for the prevention and treatment of A.panax by plant extracts.The results showed that the EC50 value of V.nigrum Linn extract to A.panax was methanol extract
参考文献/References:
[1]李延华,王伟君,张兰威,等.白藜芦醇的研究现状及应用前景[J].中国酿造,2008(7):10-12.[2]祝杲阳,刘士畅,王晓明,等.白藜芦醇对几种杨树病原真菌的抑制活性[J].安徽农业科学,2009,37(36):18224-18226,18246.[3]李巍伟.白藜芦醇抑菌作用及其机制研究[D].石家庄:河北医科大学,2008.[4]吴翠霞,王金信,侯珍,等.白藜芦醇及其衍生物对植物病原真菌的抑菌活性[J].农药学学报,2012,14(3):283-290.[5]耿艳辉,李梦青,刘桂敏,等.从虎杖中提取白藜芦醇的工艺研究[J].应用化工,2005,34(11):708-710.[6]李胜华,伍贤进.超声波提取虎杖中白藜芦醇的优化工艺研究[J].食品工业科技,2008(7):162-164.[7]孙勇民,岳鹍,王芃.复合酶法提取虎杖中白藜芦醇的工艺研究[J].食品研究与开发,2013,34(17):34-37.[8]陶明宝,鄢玉芬,陈鸿平,等.虎杖中白藜芦醇的纯化工艺研究[J].成都中医药大学学报,2018,41(1):38-41.[9]陶明宝,鄢玉芬,张乐,等.虎杖中白藜芦醇的酶法提取工艺研究[J].中药与临床,2017,8(6):34-38.[10]王莹,李梦耀,马岚,等.虎杖中白藜芦醇的提取与纯化[J].应用化工,2014,43(1):128-131.[11]叶秋雄,黄苇.虎杖中白藜芦醇提取工艺研究[J].现代食品科技,2013,29(6):1324-1327,1301.[12]陈帅,王慧竹,王万超,等.酶联半仿生法提取虎杖中白藜芦醇工艺研究[J].吉林化工学院学报,2015,32(11):20-24,41.[13]冯艳芸,岳田利,王周利.葡萄皮中白藜芦醇提取工艺优化[J].农产品加工,2015(12):35-38.[14]钱时权,石亚中,伍亚华,等.纤维素酶-超声波辅助有机溶剂提取山葡萄渣中白藜芦醇的研究[J].食品工业科技,2012,33(3):210-213.[15]王周利,袁亚宏,刘宇璇,等.响应面法优化β-环糊精提取葡萄叶白藜芦醇工艺[J].食品科学,2016,37(22):13-19.[16]范秀文,高明波,曹雪杰,等.响应面优化设计提取葡萄籽中的白藜芦醇[J].广州化工,2016,44(15):73-76,79.[17]郭燕,田丽丽,臧雪君.优化葡萄皮中白藜芦醇提取工艺研究[J].皮革与化工,2016,33(6):28-34.[18]陈琼玲,刘红芝,刘丽,等.高压-微波法提取花生根中白藜芦醇[J].食品科学,2013,34(20):43-48.[19]徐杰,谢鑫,郑祥清,等.花生芽中白藜芦醇的提取和含量分析[J].广东化工,2018,45(12):62-63.[20]安宝树,沙见浩,罗星.花生植株白藜芦醇提取工艺的优化[J].贵州农业科学,2017,45(6):114-118.[21]杨伟强,李鹏,王凤舞.碱法提取花生红衣中白藜芦醇的研究[J].食品工业科技,2009,30(4):250-251,254.[22]孙慧,林强,魏微.响应面法优化花生壳中白藜芦醇提取工艺及其抗氧化活性研究[J].应用化工,2018,47(5):867-871,878.[23]王月珍,汪岩,马千里,等.响应面法优化酶解提取花生根茎中白藜芦醇[J].食品研究与开发,2016,37(1):134-137.[24]朱祥瑞,杨逸文,占鹏飞,等.桑椹采摘后白藜芦醇含量的变化[J].蚕桑通报,2013,44(1):32-34.[25]陈琼玲,薛霖莉,樊迎,等.响应面法优化桑葚果渣中白藜芦醇的提取工艺[J].山西农业科学,2016,44(6):836-839,856.[26]曹喜涛,赵利安,屠洁,等.响应面法优化桑椹白藜芦醇的提取工艺条件[J].蚕业科学,2019,45(1):108-115.[27]樊迎,陈毅,薛霖莉,等.酶法辅助提取海红果中的白藜芦醇[J].山西农业科学,2018,46(5):819-822.[28]樊迎,陈毅,戴铭成,等.微波辅助提取海红果中的白藜芦醇[J].食品工程,2018(3):15-17,65.[29]张思思,王斌,宋志伟,等.紫斑牡丹籽中白藜芦醇超声提取工艺的优化研究[J].广州化工,2018,46(15):93-95.[30]张恒,高丽娟,王怡宁,等.酶解法提取爬山虎根皮中白藜芦醇的工艺研究[J].湖北农业科学,2016,55(2):449-452.[31]王怡宁,高丽娟,张恒,等.正交设计法优化爬山虎根皮中白藜芦醇的浸提工艺[J].辽宁科技大学学报,2015,38(5)：347-350.
相似文献/References:
[1]李海平,何玥,闫军,等.响应面法优化超声提取地皮菜多糖工艺研究[J].北方园艺,2013,37(04):138.
[2]刘伟,杨如箴,李紫薇,等.响应面法优化新疆野酸梅果肉多酚提取工艺研究[J].北方园艺,2014,38(04):123.
LIU Wei,YANG Ru-zhen,LI Zi-wei,et al.Optimization of Polyphenol From Xinjiang Wild Plum Flesh Using Response Surface Methodology[J].Northern Horticulture,2014,38(17):123.
[3]赵娟娟.响应面法优化提取菠菜中黄酮类物质的工艺研究[J].北方园艺,2013,37(14):137.
ZHAO Juan-juan.Study on Optimization Extraction Conditions of Flavonoid from Spinach by RSM[J].Northern Horticulture,2013,37(17):137.
[4]邓珂,张玉薇,张倩.基于计算机模拟的香菜保鲜响应面分析法优化[J].北方园艺,2014,38(06):140.
DENG Ke,ZHANG Yu-wei,ZHANG Qian.Response Surface Methodology of Parsley Preservation Based on Computer Simulation[J].Northern Horticulture,2014,38(17):140.
[5]高德强,王乃江,刘建军.厚朴人工林优树选择研究[J].北方园艺,2013,37(17):161.
GAO De-qiang,WANG Nai-jiang,LIU Jian-jun.Study on Plus Tree Selection Technology of Magnolia officinalis Plantation[J].Northern Horticulture,2013,37(17):161.
[6]张先廷,芦婧,王迎进.响应面法优化超声提取壶瓶枣多糖工艺研究[J].北方园艺,2013,37(08):150.
ZHANG Xian-ting,LU Jing,WANG Ying-jin.Study on Optimization of Ultrasonic-assisted Extraction Technology of Polysaccharide from Zizyphus jujuba cv.Huping by Response Surface Methodology[J].Northern Horticulture,2013,37(17):150.
[7]杨媛,石磊,张佳,等.分散固相萃取-高效液相色谱法测定草莓中游离鞣花酸含量[J].北方园艺,2012,36(15):25.
YANG Yuan,SHI Lei,ZHANG Jia,et al.Determination of Ellagic Acid in Strawberry by Dispersive Solid Phase Extraction-High Performance Liquid Chromatography[J].Northern Horticulture,2012,36(17):25.
[8]贺伟强,巢新冬,白杰.香菇中多酚类物质提取工艺的优化研究[J].北方园艺,2013,37(18):125.
HEWei-qiang,CHAOXin-dong,BAIJie.StudyontheOptimizationoftheExtractionTechnologyof TotalPhenolicfromLentinulaedodes[J].Northern Horticulture,2013,37(17):125.
[9]魏增云,张海容,陈金娥,等.微波辅助提取苦荞壳总黄酮及其抗氧化性研究[J].北方园艺,2014,38(20):144.
WEI Zeng-yun,ZHANG Hai-rong,CHEN Jin-e,et al.Study on the Microwave-assisted Extraction Total Flavonoids of Tatary Buckwheat Shell and Its Antioxidation Capacity[J].Northern Horticulture,2014,38(17):144.
[10]张敏,刘刚,张雁南,等.响应面法优化蓝靛果花色苷提取工艺研究[J].北方园艺,2014,38(23):113.
ZHANG Min,LIU Gang,ZHANG Yan-nan,et al.Optimization of Extraction of Lonicera edulis Anthocyanin by Response Surface Methodology[J].Northern Horticulture,2014,38(17):113.
备注/Memo
第一作者简介：赵玉婷（1994-），女，蒙古族，吉林前郭人，硕士研究生，研究方向为人参病害生物防治。E-mail：550707026@qq.com.责任作者：李翔国（1980-），男，朝鲜族，吉林延吉人，博士，讲师，现主要从事人参栽培等研究工作。E-mail：2420187676@qq.com.基金项目：吉林省教育厅“十三五”科学研究规划资助项目（JJKH20180886KJ）。收稿日期：2019-11-06