WEI Zeng-yun,ZHANG Hai-rong,CHEN Jin-e,et al.Study on the Microwave-assisted Extraction Total Flavonoids of Tatary Buckwheat Shell and Its Antioxidation Capacity[J].Northern Horticulture,2014,38(20):144-149.
微波辅助提取苦荞壳总黄酮及其抗氧化性研究
- Title:
- Study on the Microwave-assisted Extraction Total Flavonoids of Tatary Buckwheat Shell and Its Antioxidation Capacity
- 文章编号:
- 1001-0009（2014）20-0144-06
- Keywords:
- tatary buckwheat shell; response surface analysis method; total flavonoids; microwave-assisted extraction; antioxidation capacity
- 分类号:
- TS 201.2
- 文献标志码:
- A
- 摘要:
- 以山西灵丘苦荞壳干燥粉末为试材，利用微波辅助提取技术对苦荞壳总黄酮的提取工艺进行研究,在单因素试验的基础上，选择不同乙醇浓度、时间、液固比、功率4因素，通过Box-Behnken中心组合设计试验和响应面分析法，模拟得到二次多项式回归方程的预测模型；并以叔丁基对苯二酚为对照品，采用DPPH法研究不同方法苦荞壳黄酮提取物对自由基的清除作用。结果表明：苦荞壳总黄酮的最佳微波提取工艺为：乙醇浓度57.54%、微波时间41.09 s、液固比65.4∶1 mL/g、微波功率264.16 W，总黄酮得率实测值为1.9800%，预测值为2.004%；苦荞壳黄酮提取物具有较强的抗氧化作用，不同方法提取液在对DPPH的清除率为50%时，苦荞壳黄酮浓度为0.96 μg/mL（超声波法）、1.40 μg/mL(微波法)、1.27 μg/mL(索氏法)，而叔丁基对苯二酚的浓度为2.40 μg/mL（CK）,试验表明苦荞壳黄酮是一种很有前途的天然抗氧化剂。
- Abstract:
- Taking dry powder of tatary buckwheat shell from Lingqiu of Shanxi as malerial,the total flavonoids of tatary buckwheat shell was studied with the microwave-assisted extraction technology.The quadratic polynomial regression equation prediction model was obtained by using the Box-Behnken central composite test and response surface analysis based on the single factor experiments,such as different alcohol concentration (A),time (B),liquid-solid ratio (C),and the microwave power(D).The free radical scavenging rates of the flavonoids of tatary buckwheat shell using DPPH was tested with tert-butyl hydroquinone as the contrast.The results showed that the optimal conditions of microwave-assisted extraction of the total flavonoids were as follows：57.54% of solvent concentration,time of 41.09 s,65.4∶1 mL/g of the ratio of liquid to solid,power of 264.16 W,the yield of total flavonoids was up to 1.9800%,the predictive was of 2.004%.The flavonoids of tatary buckwheat shell had a strong antioxidant effect,the scavenging rates of tatary buckwheat shell flavonoids was up to 50% to DPPH,C50% of tatary buckwheat shell flavonoids were at 0.96 μg/mL with ultrasonic method,1.40 μg/mL with microwave-assisted extraction,1.27 μg/mL with soxhlet extraction，and 2.40 μg/mL of the tert-butyl hydroquinone(CK),respectively.The tatary buckwheat shell flavonoids was a promising natural antioxidant.
参考文献/References:
[1]谭萍,方玉梅,王毅红,等.苦荞种子黄酮类化合物清除DPPH自由基作用[J].食品研究与开发,2008,29(12):20-23.
[2]徐宝才,丁霄.苦荞壳中黄酮提取工艺的研究[J].食品工业科技,2002,23(8):40-43.
[3]唐宇,王安虎.苦荞的成分功能研究与开发应用[J].四川农业大学学报,2001(4):12-13.
[4]Cao W,Chen W J,Suo Z R,et al.Protective effects of ethanolic extracts of buckwheat groats on DNA damage caused by hydroxyl radicals[J].Food Research International,2008,41(9):924-929.
[5]Wang A M,Zhang F K,Huang L F,et al.New progress in biocatalysis and biotransformation of flavonoids[J].Jourrnal of Medicinal Plants Research,2010,4(10):847-856.
[6]Calderon-Montano J M,Burgos-Moron E,Perez-Guerrero C,et al.A Review on the Dietary Flavonoid Kaempferol[J].Mini-Reviews in Medicinal Chemistry,2011,11(4):298-344.
[7]林汝法,周小理,任贵兴,等.中国荞麦的生产与贸易、营养与食品[J].食品科学,2005,26(1):259-263.
[8]谢明勇，陈奕.微波辅助萃取技术研究进展[J].食品与生物技术学报,2006,25(1):105-114.
[9]苏秀芳,陈明兰,农克良.超声波辅助提取人面子叶总黄酮的工艺研究[J].中药材,2010,33(12):1962-1964.
[10]彭彪,王梅英,陈绍军,等.正交实验优化岩茶黄酮微波提取工艺[J].食品研究与开发,2008(2):15-18.
[11]何书美,刘敬兰.茶叶中总黄酮含量测定方法的研究[J].分析化学,2007,35(9):1365-1368.
[12]王迎进,于蕾,闫军,等.超声提取酸枣叶中总黄酮[J].食品与发酵工业,2012,38(2):229-232.
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备注/Memo
第一作者简介：魏增云（1975-），男，硕士，副教授，研究方向为天然植物分离及分析。E-mail:vzhr@163.com.
基金项目：山西省高等学校科技创新资助项目（20141120）；忻州职业技术学院2013年度立项课题资助项目（XZYJ1312）；忻州师范学院应用化学创新实践基地资助项目（2013-31）；山西省教育厅强校工程资助项目（2012089）；山西省忻州市科技研发资助项目(201231-20)。
收稿日期：2014-04-18