YIN Zhe,WEN Qilong,CAI Danzhao.Extraction and Antioxidant Activity of Polysaccharide From Tetrastigma hemsleyanum[J].Northern Horticulture,2020,44(22):96-103.[doi:10.11937/bfyy.20193908]
三叶青多糖的提取与抗氧化活性的研究
- Title:
- Extraction and Antioxidant Activity of Polysaccharide From Tetrastigma hemsleyanum
- 文献标志码:
- A
- 摘要:
- 以三叶青为试材，采用水提法提取可溶性多糖，并在单因素试验基础上做L9(34)正交实验，优化提取工艺参数，通过测定三叶青多糖的总抗氧化能力、还原力，以及清除1，1-二苯基-2-三硝基苯肼(DPPH)自由基、羟基自由基、超氧阴离子自由基的能力，研究其抗氧化作用。结果表明：在料液比1∶35 g·mL-1、提取次数1次、提取时间1.5 h、提取温度90 ℃时，三叶青多糖的提取率最高为7.34%。总抗氧化活力为88.96 U·mL-1，且具有一定的还原力；对DPPH、羟基、超氧阴离子自由基的清除率分别为36.8%、65.1%、36.8%。综上所述，料液比1∶35 g·mL-1、提取次数1次、时间1.5 h、温度90 ℃为三叶青多糖提取的最佳工艺参数。在该参数下，三叶青中提取的多糖含量最高，且具有一定的抗氧化作用。
- Abstract:
- The soluble polysaccharide was extracted by water extraction method and the extraction process parameters of L9(34) were conducted on the basis of single factor experiment.The antioxidant activity of polysaccharides was studied by measuring the total antioxidant capacity and reducing power of polysaccharides and their ability to remove 1,1-diphenyl 2-trinitrophenylhydrazine (DPPH) radical,hydroxyl radical and superoxide anion radical.The results showed that when the ratio of solid to liquid,extraction times,extraction time and extraction temperature were 1∶35 g·mL-1,once,1.5 hours and 90 ℃ respectively,the extraction rate of polysaccharide was the highest,which was 7.34%.Its total antioxidant activity unit was 88.96 U·mL-1,and it had a certain reduction force.The scavenging rates of DPPH,hydroxyl and superoxide anion radical were 36.8%,65.1% and 36.8%,respectively.The optimum process parameters for polysaccharide extraction were the ratio of solid to liquid,the numbers of extraction,extraction time and extraction temperature of 1∶35 g· ml-1,once,1.5 hours and 90 ℃,respectively.Under this parameter,the polysaccharide content extracted from clover was the highest and had certain antioxidant effect.
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备注/Memo
第一作者简介：银喆（1994-），女，硕士研究生，研究方向为新药开发及药理作用分子机制研究。E-mail：yinzhe19941118@163.com.责任作者：蔡丹昭（1972-），女，博士，副教授，硕士生导师，现主要从事新药开发及药理作用分子机制等研究工作。E-mail:danzhaoc@sina.com.收稿日期：2019-10-17