FU Yajuan,WU Zhiyan,QIAO Jie,et al.Molecular Cloning and Phylogenetic Analysis of Avidin Gene (Leav1) in Lentinus edodes[J].Northern Horticulture,2019,43(15):126-132.[doi:10.11937/bfyy.20190208]
香菇亲和素基因Leav1的克隆及系统进化分析
- Title:
- Molecular Cloning and Phylogenetic Analysis of Avidin Gene (Leav1) in Lentinus edodes
- 文献标志码:
- A
- 摘要:
- 采用RT-PCR技术克隆香菇（Lentinus edodes）亲和素基因（Leav1），并对不同来源的亲和素蛋白的分子特征及系统进化进行比较分析。结果表明：L.edodes亲和素Leav1大小为459 bp，编码152个氨基酸。来自细菌、真菌和脊椎动物的亲和素由126~183个氨基酸组成，相对分子质量为13.24~18.84 kDa，理论等电点为3.90~9.69，其中香菇Lentiavidin 1等电点最低，为3.9。对比不同来源亲和素的多序列，发现Avidin结构域长度为107~120个氨基酸，序列之间的相似度为26%~54%，但与生物素结合位点非常保守。系统进化分析发现，香菇Lentiavidin 1和白黄侧耳（Pleurotus cornucopiae）Tamavidin的亲缘关系较近。探明不同来源亲和素的分子特征及系统进化关系，对将来深入研究亲和素基因的生物学功能具有重要意义。
- Abstract:
- A lentiavidin 1 gene(Leav1) was identified from Lentinus edodes,based on Reverse Transcription-PCR (RT-PCR).The molecular characteristics and genetic relationship of avidins from different organisms were comparatively analyzed.The results showed that cDNA sequence of Leav1 was 459 bp,encoding a protein of 152 amino acids.The amino acid composition of avidins from bacteria,fungi and vertebrates ranged from 126 to 183.Their relative molecular weight was from 13.24 kDa to 18.84 kDa.The oretical isoelectric point were 3.90-9.69.The L.edodes Lentiavidin 1 possessed the lowest isoelectric point to date(3.90).Multiple sequence alignment revealed that the amino acids residues of avidin domains were from 107 to 120 in length.Lentiavidin 1 showed different homologies to those avidins from different organisms,ranging from 26% to 54%,but most of the conserved residues were associated with biotin binding activity.In system evolutionary relationship,L.edodes Lentiavidin was closer to Tamavidin of Pleurotus cornucopiae.The exploration of molecular characteristics and genentic raletionship of avidins are of great significance to elucidate their biological functions in the future.
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备注/Memo
第一作者简介：付亚娟（1981-），女，博士，讲师，研究方向为分子微生物学。E-mail：fuyajuan501@163.com.基金项目：河北省高校食药用菌资源开发应用技术研发中心资助项目(YF201411)；河北省人社厅引进留学人员资助项目（CN201714）；廊坊师范人才引进科研启动资助项目(0140102001)；廊坊师范学院微生物学重点学科资助项目(2015001)。收稿日期：2019-03-06