[1]吴文能,雷霁卿,李江阔,等.蓝莓采后病害病原菌分离鉴定及抑菌药剂筛选[J].北方园艺,2019,43(07):58-64.[doi:10.11937/bfyy.20182597]
　WU Wenneng,LEI Jiqing,LI Jiangkuo,et al.Pathogen Identification and Pharmaceutical Screening of Blueberry Postharvest Disease[J].Northern Horticulture,2019,43(07):58-64.[doi:10.11937/bfyy.20182597]
点击复制

蓝莓采后病害病原菌分离鉴定及抑菌药剂筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年07 页码: 58-64 栏目: 出版日期: 2019-04-15
Title:
Pathogen Identification and Pharmaceutical Screening of Blueberry Postharvest Disease
作者:
吴文能1; 雷霁卿1; 李江阔2; 杨灿1; 王瑞1
（1.贵阳学院 食品与制药工程学院，贵州 贵阳 550025；2.国家农产品保鲜工程技术研究中心（天津），天津市农产品采后生理与贮藏保鲜重点实验室，天津 300384）
Author(s):
WU Wenneng1; LEI Jiqing1; LI Jiangkuo2; YANG Can1; WANG Rui1
(1.Food and Pharmaceutical Engineering Institute,Guiyang College,Guiyang,Guizhou 550025；2.National Engineering and Technology Research Center for Preservation of Agricultural Products/Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Tianjin 300384)
关键词:
蓝莓; 采后病害; 灰葡萄孢菌; 青霉; 毒力测试
Keywords:
blueberry; postharvest disease; Botrytis cinerea; Penicillium; toxicity test
DOI:
10.11937/bfyy.20182597
文献标志码:
A
摘要:
以麻江蓝莓采后贮藏病果为试材，采用形态学、rDNA-ITS序列对比的方法，对病原菌的生物学进行鉴定，并通过离体试验的方法，研究了不同杀菌剂对蓝莓主要致病菌的室内毒力，以期为麻江蓝莓采后病害防控提供参考。结果表明：蓝莓采后腐烂病原菌菌株种类为灰葡萄孢菌(Botrytis cinerea)、青霉(Penicillium)、拟盘多毛孢(Pestalotiopsis cocculi)、香灰菌(Xylaria sp.)、木霉菌(Trichoderma longibrachiatum)、粪壳菌纲藻(Sordariomycetes sp.)，其中灰葡萄孢菌(Botrytis cinerea)、青霉(Penicillium)、拟盘多毛孢(Pestalotiopsis cocculi)为主要致病菌。通过对青霉（Penicillium）室内毒力结果表明，10余种杀菌剂中以咪鲜胺对蓝莓采后腐烂病原菌主要致病菌蓝莓采后腐烂病原表现出较强的抑菌活性，其EC50值分别为0.007 9 μg?mL-1，推荐作为蓝莓田间防控的有效药剂。
Abstract:
The diseased fruits of blueberry postharvest disease in Majiang county were used as test materials,and the biology of pathogens was identified by morphological and rDNA-ITS sequence comparison.The indoor toxicity of different fungicides to the main pathogenic bacteria of blueberry was studied in order to provide some reference for the prevention and control of postharvest diseases of blueberry in Majiang.The results showed that six kinds of fungi were responsible for fruit rot of blueberry,which were Botrytis cinerea,Penicillium,Pestalotiopsis cocculi,Xylaria sp.,Trichoderma longibrachiatum,and Sordariomycetes sp..Among them，Botrytis cinerea,Penicillium,Pestalotiopsis cocculi were the dominant pathogen.Prochloraz showed good inhibitive effect against Penicillium.The EC50 values of this fungicide to Penicillium was 0.007 9 μg?mL-1,which could be used as an effective control agent for blueberry postharvest disease in the field.

参考文献/References:

[1]李亚东,姜惠铁,张志东,等.中国蓝莓产业化发展的前景[J].沈阳农业大学学报(社会科学版),2001,3(1):39-42.[2]黄杏娥,和加卫,杨正松,等.现代蓝莓栽培技术研究概况[J].云南农业科技,2012(2):22-24.[3]梁晨,刘翠,赵洪海.蓝莓贮藏期病原菌的鉴定及病菌的温度效应[C].中国植物病理学会2010年学术年会论文集,2010:108-113.[4]周笑犁,王瑞,雷霁卿,等．蓝莓采后病原真菌分离及其生物学鉴定[J].食品科技,2015,40(9):283-293.[5]白雪,姜爱丽,胡文忠,等.采后蓝莓果实表面病原菌的分离鉴定及PCR检测[J].食品工业科技,2015,36(23):297-305.[6]郭晓月,丁雅迪,邓佳,等.蓝莓贮藏期病原菌的分离与鉴定[J].北方园艺,2015(24):104-108.[7]戴启东,李广旭,杨华,等.蓝莓采后病害的病原菌分离及发生规律的研究[J].果树学报,2016,33(10):1-14.[8]尉莹莹,梁晨,赵洪海,等.华东地区蓝莓采后病害病原鉴定及其多样性分析[J].山东农业科学,2017,49(7):112-115.[9]秦士维,夏秀英,安利佳.蓝莓果实潜伏侵染病原真菌的分离与鉴定[J].北方园艺,2017(18):41-48.[10]郜海燕,肖尚月,陈杭君,等.蓝莓采后主要病原真菌的分离鉴定与生物学特性研究[J].农业机械学报 2017,48(5):327-334.[11]阳秀莲,王伟,林树花,等.蓝莓贮藏期病原菌的分离鉴定及植物粗提物抑菌效果研究[J].食品科技,2018,43(5):41-48.[12]段爱莉,雷玉山,孙翔宇,等.猕猴桃果实贮藏期主要真菌病害的rDNA-ITS鉴定及序列分析[J].中国农业科学,2013,46(4):810-818.[13]KOICHIRO T,JOEL D,MASATOSHI N,et al.MEGA4:Molecular evolutionary genetics analysis (MEGA) software version 4.0[J].Molecular Biology and Evolution,2007,24(8):1596-1599.[14]范昆,张雪丹,余贤美,等.无花果炭疽病菌的生物学特性及8种杀菌剂对其抑制作用[J].植物病理学报,2013,43(1):75-81.[15]XIAO C L,ROGERS J D,KIM Y K,et al.Phacidiopycnis washingtonensis a new species associated with pome fruits from Washington State[J].Mycologia,2005,97(2):464-473.[16]KIM Y K,XIAO C L.A postharvest fruit rot in apple caused by Phacidiopycnis washingtonensis[J].Plant Disease,2006,90(11):1376-1381.[17]高鹏,南志兵,吴永娜,等.罗布麻锈病原菌鉴定[J].植物保护学报,2017,44(1):129-136.[18]TOURNAS V H,KATSOUDAS E.Mould and yeast flora in fresh berries,grapes and citrus fruits[J].International Journal of Food Microbiology,2005,105(1):11-17.[19]BARRAU C,SANTOS B D L,ROMERO F.Susceptibility of southern highbush and rabbiteye blueberry cultivars to postharvest diseases in Huelva,Spain[J].Journal of Physiology,2006,366(1):197-220.[20]MEHRA L K,MACLEAN D D,SHEWFELT R L,et al.Effect of postharvest biofumigation on fungal decay,sensory quality,and antioxidant levels of blueberry fruit[J].Postharvest Biology & Technology,2013,85:109-115.[21]MEHRA L K,SAVELLE A T,MACLEAN D D,et al.Postharvest disease development on southern highbush blueberry fruit in relation to berry flesh type and harvest method[J].Plant Disease,2013,97(2):213-221.[22]ZHU X Q,XIAO C L.Phylogenetic,morphological,and pathogenic characterization of Alternaria species associated with fruit rot of blueberry in California[J].Phytopathology,2015,105(12):1555-1567.[23]RIVERA S A,ZOFFOLI J P,LATORRE B A.Determination of optimal sulfur dioxide time and concentration product for postharvest control of gray mold of blueberry fruit[J].Postharvest Biology & Technology,2013,83(2):40-46.

相似文献/References:

[1]王明洁,吴雨蹊,段亚东,等.蓝莓栽培土壤改良技术研究[J].北方园艺,2014,38(14):179.
　WANG Ming-jie,WU Yu-xi,DUAN Ya-dong,et al.Improvement Research of Technique on Cultivation Soil of Blueberry[J].Northern Horticulture,2014,38(07):179.
[2]王贺春.蓝莓冬季修剪技术[J].北方园艺,2013,37(13):58.
　WANG He-chun.Winter Pruning Techniqne of Blueberry[J].Northern Horticulture,2013,37(07):58.
[3]聂飞,张玉春.我国蓝莓产业发展的机遇与挑战[J].北方园艺,2014,38(04):165.
　NIE Fei,ZHANG Yu-chun.Opportunities and Challenges for the Development of Blueberry Industry in China[J].Northern Horticulture,2014,38(07):165.
[4]杨芩,任永权,廖优江,等.五个兔眼蓝莓品种有效可授期研究[J].北方园艺,2013,37(14):5.
　YANG Qin,REN Yong-quan,LIAO You-jiang,et al.Study on Effective Pollination Period of Five Cultivars of Rabbiteye Blueberry[J].Northern Horticulture,2013,37(07):5.
[5]傅 冰,洪 震,刘跃钧,等.蓝莓SRAP-PCR反应体系的建立和优化及其遗传多样性分析[J].北方园艺,2013,37(17):95.
　FU Bing,HONG Zhen,LIU Yue-jun,et al.Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity[J].Northern Horticulture,2013,37(07):95.
[6]张文英,周 婧.低糖蓝莓番茄复合果酱的研制[J].北方园艺,2013,37(17):142.
　Brewing Technology Engineering Research Center,Department of Food Science,Jilin Agricultural Science and Technology College,et al.Preparation of Low-sugar Jam with Blueberry and Tomato[J].Northern Horticulture,2013,37(07):142.
[7]龚 娜,肖 军,杨 镇,等.不同菌根真菌对蓝莓根际土壤微生物数量的影响[J].北方园艺,2013,37(17):175.
　GONG Na,XIAO Jun,YANG Zhen,et al.Effects of Different Mycorrhizal Fungi on Soil Microbial Number in the Blueberry Rhizosphere[J].Northern Horticulture,2013,37(07):175.
[8]王贺春.适于丹东地区温室栽培的蓝莓引种与筛选[J].北方园艺,2013,37(12):33.
　WANG He-chun.Introduction and Selection of Blueberry Varieties for Greenhouse That Suitable for Dandong Region[J].Northern Horticulture,2013,37(07):33.
[9]吴立仁,段亚东,杜汉军,等.黑龙江省蓝莓种植应该注意的几个问题[J].北方园艺,2013,37(05):50.
　WU Li-ren,DUAN Ya-dong,DU Han-jun,et al.Several Problems Existing in Planting of Blueberry in Heilongjiang Province[J].Northern Horticulture,2013,37(07):50.
[10]刘 波.果胶酶对蓝莓果汁澄清效果的研究[J].北方园艺,2014,38(03):127.
　LIU Bo.Study on Clarification Effect of Pectinase on Blueberry Juice[J].Northern Horticulture,2014,38(07):127.

备注/Memo

第一作者简介：吴文能（1983-），男，博士，副教授，现主要从事农作物病虫害综合防治等研究工作。E-mail：wuwenneng123@126.com.责任作者：王瑞（1979-），男，博士，教授，现主要从农产品贮藏与保鲜等研究工作。E-mail：wangrui961@gmail.com.基金项目：贵州省普通高等学校科技拔尖人才支持计划资助项目（黔教合KY字[2016]091）；贵阳学院人才引进基金资助项目；大学生创新创业训练计划资助项目（201610976051）；贵州省百人计划资助项目（20164022）。收稿日期：2018-09-12

更新日期/Last Update: 2019-05-05