[1]包蕊,刘俊频,袁元,等.膜荚黄芪查尔酮还原酶基因的克隆与表达分析[J].北方园艺,2019,43(06):141-146.[doi:10.11937/bfyy.20182579]
　BAO Rui,LIU Junpin,YUAN Yuan,et al.Cloning and Expression of CHR Gene in Astragalus membranaceus[J].Northern Horticulture,2019,43(06):141-146.[doi:10.11937/bfyy.20182579]
点击复制

膜荚黄芪查尔酮还原酶基因的克隆与表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 43卷 期数: 2019年06 页码: 141-146 栏目: 出版日期: 2019-03-30
Title:
Cloning and Expression of CHR Gene in Astragalus membranaceus
作者:
包蕊; 刘俊频; 袁元; 于洋; 全雪丽; 吴松权
（延边大学 农学院，吉林 延吉 133000）
Author(s):
BAO Rui; LIU Junpin; YUAN Yuan; YU Yang; QUAN Xueli; WU Songquan
(Agricultural College,Yanbian Uniersity，Yanji，Jilin 133000)
关键词:
膜荚黄芪; 查尔酮还原酶; 基因克隆; 表达分析
Keywords:
Astragalus membranaceus (Fisch.) Bge.; chalcone reductase; gene cloning; expression analysis
DOI:
10.11937/bfyy.20182579
文献标志码:
A
摘要:
从膜荚黄芪中克隆了CHR基因，GenBank登陆号为KY086286（AmCHR），AmCHR全长cDNA为1 173 bp，开放阅读框为957 bp，编码318个氨基酸，其分子量为35.58 kDa，等电点为 6.26。生物信息学分析表明AmCHR蛋白与豆科植物CHR同源，无信号肽，定位于细胞质。实时荧光定量PCR和高效液相色谱分析结果显示AmCHR在根、茎、叶中的表达水平与毛蕊异黄酮葡萄糖苷的含量变化一致。该研究从膜荚黄芪中克隆了1个新的AmCHR基因，推测AmCHR的表达与膜荚黄芪毛蕊异黄酮葡萄糖苷的积累密切相关。
Abstract:
A CHR gene was cloned from Astragalus membranaceus in the present study，the GenBank accession number was KY086286 (AmCHR)，the full-length cDNA of AmCHR was 1 173 bp and it consisted of 957 bp open reading frame encoding 318 amino acids，which predicated molecular weight was 35.58 KDa and isoelectric point was 6.26.Bioinformatics analysis showed that AmCHR was homology with other plant CHR.Real-time PCR and HPLC indicated that the expression levels of AmCHR was similar to the changes of CG contents in different tissues.These results showed that the cloned AmCHR was a new CHR gene and the expression of AmCHR might be relevant to CG accumulation in A.membranaceus.

参考文献/References:

[1]国家药典委员会.中国药典[S].一部.北京:中国医药科技出版社,2015.[2]李延勋，栗章彭，颜世利，等.膜荚黄芪化学成分研究[J].中草药，2017，48(13):409-411.[3]MA C H，WANG R R，TIAN R R，et al.Calycosin 7-O-β-D-glucopyranoside，an anti-HIV agent from the roots of Astragalus membranaceus var.mongholicus[J].Chem Nat Compd+，2009，45(2):282-285.[4]刘江，陈沛，邢光南，等.大豆查尔酮还原酶基因GmCHR的克隆与植物表达载体构建[J].大豆科学，2013，32(2):139-142.[5]XU R R，NAN P，PAN H，et al.Molecular cloning，characterization and expression of a chalcone reductase gene from Astragalus membranaceus Bge.var.mongholicus (Bge.) Hsiao[J].Mol Biol Rep，2012,39:2275-2283.[6]李子羊，刘佳，孙海燕，等.IBA浓度对膜荚黄芪不定根生物量、毛蕊异黄酮及毛蕊异黄酮葡萄糖苷积累的影响[J].延边大学农学学报，2016，38(3):209-213.[7]BALLANCE G M，DIXON R A.Medicago sativa cDNAs encoding chalcone reductase[J].Plant Physiol,1995，107(3):1027-1028.[8]JOUNG J Y，KASTHURI G M，PARK J Y，et al.An overexpression of chalcone reductase of Pueraria montana var.lobata alters biosynthesis of anthocyanin and 5′-deoxyflavonoids in transgenic tobacco[J].Biochem Biophys Res Commun,2003，303(1):326-331.[9]PAN H，WANG Y，ZHANG H,et al.Phenylalanine ammonia lyase functions as a switch directly controlling the accumulation of calycosin and calycosin-7-O-β-D-glucoside in Astragalus membranaceus var.mongholicus plants[J].J Exp Bot，2008，59(11):3027-3037.[10]SEPIOL C J,YU J，DHAUBHADEL S.Genome-wide identification of chalcone reductase gene family in soybean:Insight into root-specific GmCHRs and Phytophthora sojae resistance[J].Front Plant Sci，2017(8):2073.[11]GOORMACHTIG S，LIEVENS S，HERMAN S，et al.Chalcone reductase-homologous transcripts accumulate during development of stem-borne nodules on the tropical legume Sesbania rostrate[J].Planta,1999，209(1):45-52.

相似文献/References:

[1]王树斌,全雪丽,周玉红,等.膜荚黄芪子叶节植株再生体系的建立[J].北方园艺,2012,36(06):177.
　WANG Shu-bin,QUAN Xue-li,ZHOU Yu-hong,et al.Establishment of Plantlet Regeneration System of Cotyledon Node for Astragalus membranaceus[J].Northern Horticulture,2012,36(06):177.
[2]王萌,王景然,李德阳,等.长白山区野生膜荚黄芪种质资源的SRAP分析[J].北方园艺,2016,40(21):94.[doi:10.11937/bfyy.201621024]
　WANG Meng,WANG Jingran,LI Deyang,et al.SRAP Analysis of Germplasm Resources of Wild Astragalus membranaceus in Changbai Moutain[J].Northern Horticulture,2016,40(06):94.[doi:10.11937/bfyy.201621024]
[3]付爽,汪洋,吕爽,等.低温对膜荚黄芪不定根总黄酮积累的影响[J].北方园艺,2017,41(22):154.[doi:10.11937/bfyy.20170244]
　FU Shuang,WANG Yang,LYU Shuang,et al.Effects of Low Temperature on Accumulation of Total Flavonoids inAdventitious Roots of Astragalus membranaceus[J].Northern Horticulture,2017,41(06):154.[doi:10.11937/bfyy.20170244]
[4]汪洋,于垚,袁宇含,等.膜荚黄芪种子中花青素提取方法的优化[J].北方园艺,2019,43(08):125.[doi:10.11937/bfyy.20182917]
　WANG Yang,YU Yao,YUAN Yuhan,et al.Optimization of Extraction Process of Anthocyanin in Seed of Astragalus membranaceus[J].Northern Horticulture,2019,43(06):125.[doi:10.11937/bfyy.20182917]
[5]郑涵予,金河延,冯艺川,等.氮和磷对膜荚黄芪不定根芒柄花苷积累的影响[J].北方园艺,2020,44(07):137.[doi:10.11937/bfyy.20192687]
　ZHENG Hanyu,JIN Heyan,FENG Yichuan,et al.Effects of Nitrogen and Phosphorus on the Accumulation of Arbutin in Adventitious Roots of Astragalus membranaceus[J].Northern Horticulture,2020,44(06):137.[doi:10.11937/bfyy.20192687]
[6]李晶晶,冉盼,王佳,等.膜荚黄芪组织培养及植株再生体系的建立[J].北方园艺,2024,(14):109.[doi:10.11937/bfyy.20234354]
　LI Jingjing,RAN Pan,WANG Jia,et al.Tissue Culture and Establishment of Regeneration System of Astragalus membranaceus[J].Northern Horticulture,2024,(06):109.[doi:10.11937/bfyy.20234354]

备注/Memo

第一作者简介：包蕊（1994-），女，硕士研究生，研究方向为特种植物资源。E-mail:1261731745@qq.com.责任作者：全雪丽（1973-），女，博士，教授，研究方向为植物学。E-mail:qxl2000@aliyun.com.基金项目：国家自然科学基金资助项目（21462044,30860036）；吉林省自然科学基金资助项目（201115228）。收稿日期：2018-09-05

更新日期/Last Update: 2019-04-22