WANG Meng,WANG Jingran,LI Deyang,et al.SRAP Analysis of Germplasm Resources of Wild Astragalus membranaceus in Changbai Moutain[J].Northern Horticulture,2016,40(21):94-97.[doi:10.11937/bfyy.201621024]
长白山区野生膜荚黄芪种质资源的SRAP分析
- Title:
- SRAP Analysis of Germplasm Resources of Wild Astragalus membranaceus in Changbai Moutain
- Keywords:
- Astragalus membranaceus; SRAP; genetic diversity; germplasm
- 文献标志码:
- A
- 摘要:
- 以野生膜荚黄芪的叶片为试材，采用SRAP分子标记技术，研究了长白山区野生膜荚黄芪种质资源的遗传多样性，以期为膜荚黄芪种质资源的保存和优良品种的选育奠定基础。结果表明：从300对引物组合中选出扩增产物条带多，特征性强，重复性明显的16对引物组合；利用这些引物进行PCR扩增，共得到129条条带，其中多态性条带数为95条。聚类分析结果表明，长白山区野生膜荚黄芪种质资源之间存在着丰富遗传差异。
- Abstract:
- Taking leaves of wild Astragalus membranaceus as test materials，genetic diversity of germplasm resources of wild A.membranaceus in Changbai Moutain was studied by using SRAP molecular markers，in order to provide a foundation for preserving and breeding good varieties for Astragalus membranaceus.The results showed that 16 pairs of primers，which could produce more，characteristic and reproducible bands，were screened from 300 pairs of SRAP primers；these primes amplified 129 bands from which polymorphic bands were 95 bands.The cluster analysis disclosed that there were abundant genetic differences among the wild resources of A.membranaceus in Changbai Moutain，which was beneficial for cross-breeding of A.membranaceus.
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备注/Memo
第一作者简介：王萌（1991-），男，山东临沂人，硕士研究生，研究方向为特种植物资源与生物技术。E-mail：718213098@qq.com.责任作者：吴松权（1972-），男，黑龙江鸡西人，博士，副教授，现主要从事特种植物资源与生物技术等研究工作。E-mail：arswsq@ybu.edu.cn.基金项目：国家自然科学基金资助项目（21462044）。