DONG Shanshan,YANG Haiyan,WU Wenlong,et al.Study on the Synthesis of Vitamin E in Different Blackberry Varieties[J].Northern Horticulture,2019,43(08):26-31.[doi:10.11937/bfyy.20182520]
不同黑莓品种果实发育过程中生育酚合成规律研究
- Title:
- Study on the Synthesis of Vitamin E in Different Blackberry Varieties
- Keywords:
- blackberry; fruit development; vitamin E
- 文献标志码:
- A
- 摘要:
- 以生产上表现优异的黑莓品种“宁植3号”和“Kiowa”为试验材料，采用高效液相色谱法（HPLC），在花后14 d直至果实成熟过程中对黑莓种子及全果中生育酚含量和组分进行测定分析，以期为黑莓的进一步开发利用提供参考。结果表明：随着果实的发育，“宁植3号”和“Kiowa”种子中α-生育酚含量总体呈下降趋势，β+γ-和δ-生育酚含量呈上升趋势，生育酚总量不断上升；全果中α-生育酚含量亦呈现持续下降的趋势，β+γ-和δ-生育酚含量随着果实发育先升后降；全果和种子中不同组分生育酚的变化规律不一致，种子和全果中β+γ-生育酚积累量最多，α-生育酚含量次之，分析认为这可能是由不同的候选基因决定。
- Abstract:
- The synthesis and changes of vitamin E during blackberry fruit development were investigated in this study.The blackberry cultivars ‘Ningzhi 3’ and ‘Kiowa’ were used as the research materials,and the high-performance liquid chromatography (HPLC) was applied to determine the total amount of vitamin E and components in blackberry seeds and whole fruits at 14 days after flowering,and continued until the ripening of the fruit.The results showed that with the development of fruits,the contents of α-tocopherol in the seeds of the two cultivars declined,while the contents of β+γ-and δ-tocopherol increased.The total vitamin E content increased continuously,which reached the maximum on the 35th and 49th days,and the contents were 36.47 mg?(100g)-1DW and 47.66 mg?(100g)-1DW,respectively.The content of α-tocopherol in the whole fruits of the two cultivars also showed a continuous decrease trend,reaching minimum values on the 42th and 49th days,respectively,and the content of β+γ-and δ-tocopherol first increased and then decreased with fruits growth,the β+γ-tocopherol content of the two cultivars reached the peak on the 35th day,and the δ-tocopherol peaked on the 35th and the 42nd day respectively.In the early period of fruit development,the total content of vitamin E was maintained higher content in the whole fruit of both cultivars,the highest content was 77.34 mg?(100g)-1DW and 72.76 mg?(100g)-1DW on the 35th day,respectively,and then the content began to decrease after 35 days.In conclusion,the blackberry cultivars ‘Ningzhi 3’ and ‘Kiowa’ had higher content of vitamin E in the whole fruit and seeds,and the total content of vitamin E in the whole fruit was higher than that in the seeds.The changes of different tocopherols components in the whole fruit and seeds were different.The accumulation of β+γ-tocopherol was the highest in seeds and whole fruits,and the next was α-tocopherol.It was considered that this changes may be determined by different candidate genes.
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备注/Memo
第一作者简介：董珊珊（1992-），女，硕士研究生，研究方向为小浆果生理。E-mail：15305183284@163.com.责任作者：李维林（1966-），男，博士，研究员，现主要从事小浆果栽培及生理生化和分子等研究工作。E-mail:wlli@njfu.edu.cn.基金项目：江苏省科技基础设施建设资助项目（BM2015019）；江苏省植物资源研究与利用重点实验室资助项目（江苏省中国科学院植物研究所）（JSPKLB201608）；南京市科技计划资助项目（201608059）；江苏省农业三新工程资助项目（SXGC[2017]309）；国家自然科学基金资助项目（31700606）。收稿日期：2018-10-29