[1]杨海燕,张春红,吴文龙,等.黑莓果实发育过程中抗氧化系统变化规律研究[J].北方园艺,2014,38(16):13-16,17.
　YANG Hai-yan,ZHANG Chun-hong,WU Wen-long,et al.Changes on Antioxidant System in Fruit of Rubus spp.cv ‘Arapaho’ During the Development and Ripening Stage[J].Northern Horticulture,2014,38(16):13-16,17.

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黑莓果实发育过程中抗氧化系统变化规律研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷期数: 2014年16期页码: 13-16,17 栏目: 出版日期: 2014-08-30

Title:: Changes on Antioxidant System in Fruit of Rubus spp.cv ‘Arapaho’ During the Development and Ripening Stage

文章编号:: 1001-0009(2014)16-0013-05

作者:: 杨海燕; 张春红; 吴文龙; 李维林; 江苏省中国科学院植物研究所，江苏南京 210014

Author(s):: YANG Hai-yan; ZHANG Chun-hong; WU Wen-long; LI Wei-lin; Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing,Jiangsu 210014

关键词:: 黑莓; 果实发育; 抗氧化系统

Keywords:: blackberry (Rubus spp.); fruit development; antioxidant system

分类号:: S663.2

文献标志码:: A

摘要:: 摘要：以生产上表现优异的‘Arapaho’黑莓品种为试材，在花后3 d至成熟过程中对果实细胞中抗氧化系统的动态变化进行了观察测定。结果表明：黑莓果实发育过程中超氧化物歧化酶（SOD）和过氧化物酶（POD）活性变化表现为先升后降的趋势，过氧化氢酶（CAT）活性变化呈下降趋势；抗坏血酸（AsA）、脯氨酸（Pro）含量呈下降趋势，还原型谷胱甘肽（GSH）含量和超氧阴离子自由基（O^-.₂）产生速率呈先升后降的趋势，丙二醛（MDA）含量变化趋势并不明显。抗氧化酶SOD、POD、CAT和抗氧化物质AsA、GSH、Pro协同作用，使得果实一直处于活性氧产生和清除的平衡状态，从而保证了果实能够正常发育到完全成熟

Abstract:: Taking blackberry cultivar(Rubus spp.cv‘Arapaho’) as material,from the stage of three days after flower (DAF) to the maturation process,fruit antioxidant system of ‘Arapaho’ was investigated.The results showed that superoxide dismutase (SOD) and peroxydase (POD) activity first increased and then decreased,catalase (CAT) activity decreased gradually during the fruit development and ripening process;antiscorbic acid (AsA) and free proline (Pro) content decreased during the fruit ripening process,glutathione (GSH) content and the generation of O₂ first increased and then decreased,MDA content showed no significant difference in the process of fruit development.The combination of antioxidases SOD,POD,CAT and antioxidant materials AsA,GSH,Pro played an important role to keep the balance of generating and scavenging of active oxygen in the process of fruit development,and ensure fruit to develop and mature.

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备注/Memo

第一作者简介：杨海燕（1983-），女，博士，助理研究员，现主要从事黑莓等小果类生物技术研究。E-mail：haiyanyang_025@126.com.

责任作者：李维林（1966-），男，研究员，博士生导师，现主要从事悬钩子属植物（黑莓和树莓）等研究和应用工作。E-mail：lwlcnbg@mail.cnbg.net.

基金项目：江苏省科技支撑计划资助项目（BE2013440，BE2013309）；江苏省农业三新工程资助项目（SXGC［2013］347）。

更新日期/Last Update: 2014-09-25