[1]杨珊,张赫男,李巧珍,等.猴头菌融合育种原生质体制备与再生条件优化[J].北方园艺,2018,42(09):159-165.[doi:10.11937/bfyy.20173296]
　YANG Shan,ZHANG Henan,LI Qiaozhen,et al.Optimization of Protoplast Preparation and Regeneration Conditions of  Hericium erinareus Fusion Breeding[J].Northern Horticulture,2018,42(09):159-165.[doi:10.11937/bfyy.20173296]
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猴头菌融合育种原生质体制备与再生条件优化

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年09 页码: 159-165 栏目: 出版日期: 2018-04-15
Title:
Optimization of Protoplast Preparation and Regeneration Conditions of  Hericium erinareus Fusion Breeding
作者:
杨珊1; 2; 张赫男1; 李巧珍1; 吴迪1; 陈明杰1; 杨焱1
（1.农业部南方食用菌资源利用重点实验室，国家食用菌工程技术研究中心，上海市农业科学院 食用菌研究所，上海 201403；2.上海海洋大学 食品学院，上海 201306）
Author(s):
YANG Shan1; 2; ZHANG Henan1; LI Qiaozhen1; WU Di1; CHEN Mingjie1; YANG Yan1
(1.Key Laboratory of Edible Fungus Resources and Utilization (South),Ministry of Agriculture/National Engineering Research Center of Edible Fungi/Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，Shanghai 201403；2.Food College,Shanghai Ocean University，Shanghai 201306)
关键词:
猴头菌; 原生质体; 制备; 再生率
Keywords:
Hericium erinareus; protoplast; preparation; regeneration rate
DOI:
10.11937/bfyy.20173296
文献标志码:
A
摘要:
以猴头菌0605为试材，采用单因素试验和正交实验，研究了酶系统、酶解时间、酶解温度、菌龄、恒温摇床转速单因素对其原生质体制备与再生的影响。结果表明：原生质体制备和再生的最佳条件为菌龄10 d的菌丝体，在酶系统2.0%溶壁酶＋0.5%崩溃酶作用下，32 ℃酶解2.5 h，恒温摇床转速为120 r?min-1。该试验在最佳条件下获得的猴头菌原生质体数量与再生率均较高，为进一步开展猴头菌原生质体融合育种奠定基础。
Abstract:
Hericium erinareus 0605 was used as test material，the influence of enzyme system，enzymolysis time，enzymolysis temperature，mycelial age and shaking speed on the preparation and regeneration of protoplasts of Hericium erinareus 0605 were studied by single factors and orthogonal design experiments.The results showed that the optimal preparing and regeneration conditions of Hericium erinareus protoplasts were mycelium age 10 days，using 2.0% lywallzyme and 0.5% driselase，digesting at 32 ℃，shaking speed 120 r?min-1 for 2.5 hours.Under this conditions，the study established the foundation of protoplast fusion breeding of Hericium erinareus.

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备注/Memo

第一作者简介：杨珊（1991-），女，硕士研究生，研究方向为微生物遗传育种。E-mail：1196157736@qq.com.责任作者：杨焱（1970-），女，博士，研究员，现主要从事食药用菌活性成分的分离纯化及功能产品开发等研究工作。E-mail：yangyan@saas.sh.cn.基金项目：上海市科技兴农种业资助项目（沪农科种字(2016)第1-12号)。收稿日期：2017-11-21

更新日期/Last Update: 2018-05-25