[1]张浩,叶嘉,郝立华,等.正交实验优化卷柏类黄酮提取工艺及其体外抗氧化作用[J].北方园艺,2017,41(21):134-139.[doi:10.11937/bfyy.20171938]
　ZHANG Hao,YE Jia,HAO Lihua,et al.Optimization of Extraction Process and in vitro Antioxidant Activities of Flavonoid From Selaginella tamariscina[J].Northern Horticulture,2017,41(21):134-139.[doi:10.11937/bfyy.20171938]
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正交实验优化卷柏类黄酮提取工艺及其体外抗氧化作用

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年21 页码: 134-139 栏目: 出版日期: 2017-11-15
Title:
Optimization of Extraction Process and in vitro Antioxidant Activities of Flavonoid From Selaginella tamariscina
作者:
张浩1; 2; 叶嘉1; 2; 郝立华3; 郑云普3; 董田玉1; 吴子龙1; 2
1.邯郸学院 生命科学与工程学院，河北 邯郸 056005；2.河北省高校冀南太行山区资源植物应用技术研发中心，河北 邯郸 056005；3.河北工程大学 水利水电学院，河北 邯郸 056038
Author(s):
ZHANG Hao1; 2; YE Jia1; 2; HAO Lihua3; ZHENG Yunpu3; DONG Tianyu1; WU Zilong1; 2
1.School of Life Science and Engineering,Handan University,Handan,Hebei 056005;2.Research Center for Plant Resources Applied Technology in Jinan Mountain at University of Hebei Province,Handan,Hebei 056005;3.School of Water Conservancy and Hydropower,Hebei University of Engineering,Handan,Hebei 056038
关键词:
卷柏; 类黄酮; 抗氧化活性; 正交实验设计; 超氧负离子
Keywords:
Selaginella tamariscina Spring; flavonoids; antioxidant activity; orthogonal array design; superoxide anion
DOI:
10.11937/bfyy.20171938
文献标志码:
A
摘要:
以冀南山区的卷柏为试材，通过单因素试验和L9(34)正交实验优化类黄酮提取工艺参数，并对其抗氧化活性进行检测，探究卷柏类黄酮提取的最佳工艺及其体外抗氧化效果。结果表明：在最优工艺条件乙醇体积分数60%、料液比1∶15 g?mL-1、浸提时间30 min、浸提温度70 ℃时，类黄酮的提取率为15.7%。卷柏提取液具有较好清除超氧负离子自由基的能力，当浸提物溶液浓度为3.0 mg?mL-1时，超氧阴离子清除率达到最大值69.4%。结果不仅为进一步优化类黄酮提取工艺参数提供数据参考，而且还为深入理解卷柏抗氧化机理和合理高效利用药用植物资源提供参考依据。
Abstract:
In order to investigate the optimal process parameters for extracting flavonoids from Selaginella tamariscina and its antioxidant activities,single factor experiments and an orthogonal array design L9(34) were performed in combination to optimize process parameters for the extraction of flavonoids from Selaginella tamariscina.Meanwhile,the scavenging effects of flavonoids on the superoxide anion were also examined in the current study.The results showed that the extraction yield of flavonoids was 15.7% with the optimal parameters that the ethanol concentration,solid-liquid ratio,extraction time,and extraction temperature were 60%,1∶15 g?mL-1,30 minutes,and 70 ℃,respectively.Moreover,it was found that the extraction from Selaginella tamariscina characterized by high antioxidant ability for scavenging the superoxide anion with the maximum scavenging ratio at the extraction concentration of 3.0 mg?mL-1.These results not only provided data for further optimizing the extraction parameters of flavonoids from Selaginella tamariscina,but also had important significance for understanding the antioxidant mechanisms and high efficiency utilization of plant resources.

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备注/Memo

第一作者简介：张浩 (1983-),男,硕士,讲师,研究方向为系统与进化植物学。E-mail:zhanghao_55@163.com.

责任作者:郝立华(1981-),女,硕士,讲师,研究方向为植物生理生态学。E-mail:haolihua_000@sina.com.
基金项目：国家自然科学基金青年资助项目(31400418)；河北省科技计划资助项目(12272502)；邯郸市科学技术研究与发展计划资助项目(1323108093-9)；河北省自然科学基金青年基金资助项目（C2014109013）；河北省高校冀南太行山区资源植物应用技术研发中心资助项目；河北省高等学校科学技术研究资助项目(ZD2017302)；邯郸学院校级科研资助项目(15217，15105)。
收稿日期：2017-07-10

更新日期/Last Update: 2017-11-14