YE Weizhen,LIU Shi,MA Hongyan,et al.QTL Analysis on Related Seed Traits in Melon Based on CAPS Markers[J].Northern Horticulture,2017,41(12):119-128.[doi:10.11937/bfyy.201712028]
基于CAPS标记的甜瓜种子相关性状QTL分析
- Title:
- QTL Analysis on Related Seed Traits in Melon Based on CAPS Markers
- Keywords:
- melon; seed traits; SNP; CAPS; genetic linkage map; QTL
- 文献标志码:
- A
- 摘要:
- 以种子相关性状差异显著的甜瓜品系MR-1和M1-15为试材，构建BC1P1回交群体，利用2个亲本材料全基因组重测序数据发掘SNP位点并将其转化为CAPS标记，构建遗传连锁图谱并对种子相关性状进行QTL分析。结果表明：获得了一张包含197个CAPS标记、12个连锁群的甜瓜遗传连锁图谱。检测到与种子相关性状紧密连锁的QTL位点6个，分布在1、2、5、8号染色体上，可解释9.86%~13.52%的表型变异率，贡献率＞10%的QTL位点5个。
- Abstract:
- Two melon inbred lines MR-1 and M1-15 with a significant difference in the seed traits were used as materials，to make a cross for BC1P1 genetic population.The SNP loci was developed and converted into CAPS markers based on the whole genome re-sequencing data of the two parental materials to construct genetic linkage map and QTL analysis of seed related traits.The results showed that a genetic linkage map containing 197 CAPS markers was obtained with 12 linkage groups and 6 QTLs for the seed related traits were detected and distribution in chromosome 1，2，5 and 8.The value range of phenotypic variation was from 9.86% to 13.52% and the value of five QTLs were more than 10%.
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备注/Memo
第一作者简介：叶伟震（1991-），男，硕士研究生，研究方向为西甜瓜分子遗传育种。E-mail：714859401@qq.com.责任作者：栾非时（1964-），女，教授，博士生导师，研究方向为西甜瓜分子遗传育种。