[1]姚方杰,鲁丽鑫,王鹏,等.SRAP法鉴定黑木耳菌种真实性[J].北方园艺,2017,41(24):178-182.[doi:10.11937/bfyy.20171202]
　YAO Fangjie,LU Lixin,WANG Peng,et al.Verification of Genuineness for Spawn of Auricularia heimuer by SRAP[J].Northern Horticulture,2017,41(24):178-182.[doi:10.11937/bfyy.20171202]
点击复制

SRAP法鉴定黑木耳菌种真实性

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年24 页码: 178-182 栏目: 出版日期: 2017-12-25
Title:
Verification of Genuineness for Spawn of Auricularia heimuer by SRAP
作者:
姚方杰1; 2; 鲁丽鑫2; 王鹏2; 张友民1; 姚允武3; 李宏月1
1.吉林农业大学 园艺学院,吉林 长春 130118;2.食药用菌教育部工程研究中心,吉林 长春 130118;3.吉林省海外农业投资开发集团有限公司,吉林 长春 130118
Author(s):
YAO Fangjie1; 2; LU Lixin2; WANG Peng2; ZHANG Youmin1; YAO Yunwu3; LI Hongyue1
1.College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118;2.Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Changchun,Jilin 130118;3.Jihai Agricultural Investment & Development Group Co.Ltd.,Changchun,Jilin 130118
关键词:
黑木耳; 菌种; 真实性鉴定; SRAP
Keywords:
Auricularia heimuer; spawn; verification of genuineness; SRAP
DOI:
10.11937/bfyy.20171202
文献标志码:
A
摘要:
该研究详细介绍了采用SRAP法鉴定黑木耳菌种真实性标准的具体内容，为生产者提供科学有效、简易操作的菌种鉴定方法，为育种者知识产权保护提供有效途径。
Abstract:
This study introduced detailed the specific content of the standard that the verification of genuineness for spawn of Auricularia heimuer by SRAP.This standard provided not only a scientifically effective and easy-to-use method for the verification of genuineness for spawn,but also an effective way for breeders to protect intellectual property rights.

参考文献/References:

[1]吴芳,戴玉成.黑木耳复合群中种类学名说明[J].菌物学报,2015(4):604-611.

[2]姚方杰,边银丙.图说黑木耳关键栽培技术[M].北京:中国农业出版社,2010:42.
[3]姚方杰,张友民,鲁丽鑫,等.黑木耳遗传育种研究进展[J].菌物研究,2015(3):125-128，122.
[4]张敏琴,杜才富,韩宏仕,等.应用SRAP标记鉴定油菜杂交种真实性研究初探[J].种子,2013,32(8):33-36.
[5]LI G,QUIROS C F.Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:Its application to mapping and gene tagging in Brassica[J].Theor Appl Genet,2001,103:455-461.
[6]柳李旺,龚义勤,黄浩,等.新型分子标记-SRAP与TRAP及其应用[J].遗传,2004(5):777-781.
[7]徐操,赵宝华.SRAP分子标记的研究进展及其应用[J].生命科学仪器,2009(4):24-27.
[8]李严,张春庆.新型分子标记-SRAP技术体系优化及应用前景分析[J].中国农学通报,2005,21(5):108-112.
[9]王晓娥,姚方杰,张友民,等.木耳属菌株ITS序列作为DNA条形码的可行性[J].东北林业大学学报,2013,41(7):111-114.
[10]王晓娥,姚方杰.块菌rDNA的ITS序列分析试验条件的初步研究[J].中国食用菌,2006,25(5):37-39.
[11]王凤格,易红梅,赵久然,等.玉米真实性SSR鉴定标准的研制及应用[J].玉米科学,2016,24(4):61-66.
[12]刘华晶,许修宏,姜廷波.大兴安岭地区野生黑木耳菌株SRAP的遗传多样性分析[J].中国农业科学,2011(13):2641-2649.
[13]TANG L H,XIAO Y,LI L,et al.Analysis of genetic diversity among Chinese Auricularia auricula cultivars using combined ISSR and SRAP markers[J].Current Microbiology,2010,61(2):132-140.
[14]李媛媛.SRAP标记和SCAR标记在黑木耳栽培菌株分类鉴定中的应用[D].长春:吉林农业大学,2013.
[15]李翠翠,郭立忠,卢伟东,等.RAPD和SRAP分子标记在真姬菇菌种鉴定中的应用[J].食用菌学报,2009,16(1):21-25.
[16]郭钟尧,姜辉,贾培培,等.RAPD和SRAP分子标记在金针菇菌种鉴定中的应用[J].食用菌,2011(2):15-17.
[17]谢成婕,童方丽,肖立民,等.聚丙烯酰胺凝胶垂直电泳和琼脂糖水平电泳检测基因型的比较研究[J].广东牙病防治,2011,19(8):395-397.
[18]张彦苹,曹尚银,初建青,等.16份石榴RAPD扩增产物的两种电泳方法检测及其序列特征[J].基因组学与应用生物学,2010,29(5):890-896.
[19]王爱娜,王灏,李殿荣,等.琼脂糖与聚丙烯酰胺凝胶电泳在SSR鉴定杂交油菜种子纯度中的比较[J].西北农业学报,2012(8):101-106.

相似文献/References:

[1]桂明英,赵春艳,吴素蕊,等.黑木耳18S rDNA序列变异及遗传多样性分析[J].北方园艺,2014,38(14):111.
　GUI Ming-ying,ZHAO Chun-yan,WU Su-rui,et al.Analyses of Sequence Variation and Genetic Diversity on 18S rDNA Regions of Auricularia auricula-judae[J].Northern Horticulture,2014,38(24):111.
[2]徐彦军,吴秀泉,宋世良,等.不同速生树种培养料对黑木耳生长及产量的影响[J].北方园艺,2013,37(10):143.
　XU Yan-jun,WU Xiu-quan,SONG Shi-liang,et al.Effect of Different Culture Material of Fast growing Trees on the Growth and Yield of Auricularia auricula[J].Northern Horticulture,2013,37(24):143.
[3]陈 影,姚方杰,张友民,等.黑木耳新品种选育研究[J].北方园艺,2014,38(08):133.
　CHEN Ying,YAO Fang-jie,ZHANG You-min,et al.Study on Breeding of A New Auricularia auricula-judae (Bull.) Quél.Cultivar[J].Northern Horticulture,2014,38(24):133.
[4]方华舟.蛹虫草菌种培养性状与出草结果关系的研究[J].北方园艺,2013,37(12):161.
　FANG Hua-zhou.Study on the Relationship Between Cultural Characteristics and Results of Weeds of Cordyceps militaris[J].Northern Horticulture,2013,37(24):161.
[5]何彩梅,唐政.黑木耳多糖的提取工艺研究[J].北方园艺,2013,37(07):156.
　HE Cai-mei,TANG Zheng.Study on Extraction Technology of Polysaccharides from Auricularia auricula[J].Northern Horticulture,2013,37(24):156.
[6]唐玉琴,李凤林,宫敬利.黑木耳新品种“吉耳1号”的区域试验[J].北方园艺,2012,36(02):174.
　TANG Yu-qin,LI Feng-lin,GONG Jing-li.Regional Trials of New Variety of Auricularia auricula ‘Jier No1’[J].Northern Horticulture,2012,36(24):174.
[7]刘贵巧,暴增海,薛晓畅.冀南平菇菌种资源鉴定研究[J].北方园艺,2012,36(24):162.
　LIU Gui-qiao,BAO Zeng-hai,QUE Xiao-chang.Preliminary Study on Oyster Mushroom Strain Identification in the Southern of Hebei[J].Northern Horticulture,2012,36(24):162.
[8]任思竹,郭亚萍,张国庆,等.蜜环菌菌种及配方的筛选[J].北方园艺,2013,37(18):145.
　RENSi-zhu,GUOYa-ping,ZHANGGuo-qing,et al.ScreeningonSpeciesandFormulationofArmillariamelleaStrains[J].Northern Horticulture,2013,37(24):145.
[9]魏雅冬,戴明,王相刚,等.黑木耳固体栽培基质优化研究[J].北方园艺,2013,37(16):176.
　WEIYa-dong,DAIMing,WANGXiang-gang,et al.StudyonOptimizationoftheSolidSubstratesofBlackFungus[J].Northern Horticulture,2013,37(24):176.
[10]方华舟.不同蛹虫草优质母种分离方式与菌种性能比较[J].北方园艺,2014,38(15):142.
　FANG Hua-zhou.Comparison of Separation Method and Performance Comparison Among Different High Quality Stock Culture of Cordyceps militaris[J].Northern Horticulture,2014,38(24):142.

备注/Memo

第一作者简介：姚方杰（1965-），女，博士，教授，研究方向为食用菌遗传育种与高效栽培。E-mail：yaofj@aliyun.com.

责任作者：张友民（1963-），男，博士，教授，研究方向为植物学与湿地植物生态科学。E-mail：zhangymf@aliyun.com.
基金项目：国家自然科学基金资助项目（31272219）。
收稿日期：2017-08-03

更新日期/Last Update: 2017-12-29