[1]张冬野,李会佳,杨欢欢,等.番茄裂果性状及QTL定位分析[J].北方园艺,2017,41(17):44-51.[doi:10.11937/bfyy.20171000]
　ZHANG Dongye,LI Huijia,YANG Huanhuan,et al.Fruit Cracking and QTL Mapping of Tomato[J].Northern Horticulture,2017,41(17):44-51.[doi:10.11937/bfyy.20171000]
点击复制

番茄裂果性状及QTL定位分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年17 页码: 44-51 栏目: 出版日期: 2017-09-15
Title:
Fruit Cracking and QTL Mapping of Tomato
作者:
张冬野; 李会佳; 杨欢欢; 李景富; 许向阳
东北农业大学 园艺园林学院，黑龙江 哈尔滨 150030
Author(s):
ZHANG Dongye; LI Huijia; YANG Huanhuan; LI Jingfu; XU Xiangyang
College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030
关键词:
番茄; 裂果; 相关性分析; QTL分析
Keywords:
Lycopersicon esculentum Mill.; cracking; correlation analysis; QTL mapping
DOI:
10.11937/bfyy.20171000
文献标志码:
A
摘要:
以耐裂果的加工番茄‘14803’和易裂果的栽培番茄‘14633’为试材，将二者杂交得到F1代,F1自交得到F2，研究了F2子代的裂果相关性及QTL定位，以期为近一步培育耐裂果、品质优良的新品种提供参考依据。结果表明：番茄果实抗裂指数与果实纵径、果形指数、果肩果皮韧度、果腹果皮韧度4个指标呈显著正相关。通过利用SSR和AFLP分子标记对F2群体进行QTL定位及遗传连锁图谱的构建，找到了27个与番茄果质量、果形指数、果皮韧度等相关的QTLs，5个抗裂指数相关的QTLs。
Abstract:
Resistant crack tomato ‘14803’ and easily crack tomato ‘14633’ were used as test materials,the F1 generation was obtained by hybridization of ‘14803’ and ‘14633’,the resulting F1 plants were self-crossed to obtain F2.Correlations among fruit cracking and QTL of the F2 generation were investigated in this study,in order to provide a theoretical basis for further cultivating new varieties with crack resistance and good quality.The results showed that there were significant positive correlations between the crack resistance index and fruit longitudinal diameter,fruit shape index,the toughness of the peel on the fruit shoulder and the toughness of the peel in the middle of the fruit.The SSR and AFLP techniques were used to construct the genetic linkage map and QTL mapping.A total of 27 QTLs related to fruit weight,fruit shape index and fruit toughness were detected by F2 generation,among them there were 5 QTLs related with crack resistance index.

参考文献/References:

[1]郑玲.番茄裂果的原因及防治对策[J].北京农业,2012(21):93-94.

[2]王广印,陈碧华,沈军,等.大棚秋番茄裂果原因分析与综合防治对策[J].中国园艺文摘,2014(7):162-165.
[3]苏彦宾,刘玉梅,方智远,等.结球甘蓝耐裂球性状遗传分析[J].园艺学报,2012(8):1482-1490.
[4]PRASHAR D P,LAMBETH V N.Inheritance of radial fruit cracking in tomatoes[J].Proceedings American Society for Horticultural Science，1960,76：530-537.
[5]谷婧玥.番茄果实可溶性糖含量的QTL定位及种质资源筛选[D].哈尔滨:东北农业大学,2014.
[6]VOS P,HOGERS R,BLEEKER M.AFLP：A new technique for DNA fingerprinting[J].Nucleic Acids Research,1995(23)：4407-4414.
[7]彭艳.番茄早熟性相关性分析及QTL定位[D].哈尔滨:东北农业大学,2015.
[8]陈丽静.番茄AFLP分子遗传连锁图谱的构建及抗病基因Multi-caps标记识别体系建立[D].沈阳:沈阳农业大学,2006.
[9]汝学娟,潘光辉,汝学玲,等.番茄裂果机理及防治措施[J].北方园艺,2011(15):225-227.
[10]刘仲齐,薛俊,金凤媚,等.番茄裂果与果皮结构的关系及其杂种优势表现[J].华北农学报,2007(3):141-147.
[11]BARGEL H,NEINHUIS C.Tomato (Lycopersicon esculentum Mill.) fruit growth and ripening as related to the biomechanical properties of fruit skin and isolated cuticle[J].J Experimental Botany,2005,56(413):1049-1060.
[12]REYNARD G B.Inherited resistance to radial cracks in tomato fruits[J].Proc Amer Soc Hort Sci,1951,58：231-244.
[13]YOUNG H W.Inheritance of radial fruit cracking in a tomato cross[J].Florida State Horticultural Society,1959,72:207-210.
[14]CORTS C,AYUSO M C,PALOMARES G,et al.Relationship between radial and concentric cracking of tomato fruit[J].Scientia Horticulturae,1983,21(4)：323-328.
[15]江海坤,许晓婷,王明霞,等.西瓜裂果性状QTL相关性分析[C]//中国园艺学会2014年学术年会论文摘要集.中国园艺学会,中国农业科学院蔬菜花卉研究所,2014.
[16]文雁成,傅廷栋,涂金星,等.甘蓝型油菜抗裂角品种(系)的筛选与分析[J].作物学报,2008(1):163-166.
[17]李丹.石榴优良品系筛选及遗传多样性分析[D].杨凌:西北农林科技大学,2008.
[18]杨丽,孙浩元,张俊环,等.鲜食杏裂果与果实部分性状的相关性研究[J].河南农业科学,2010(4):83-85.
[19]苏彦宾,刘玉梅,李占省,等.结球甘蓝遗传图谱构建及耐裂球QTL定位[C]//中国园艺学会2014年学术年会论文摘要集.中国园艺学会,中国农业科学院蔬菜花卉研究所,2014.

相似文献/References:

[1]吴晓燕,张丽荣,马建华,等.一种新型微生物制剂对设施番茄土壤微生物数量的影响[J].北方园艺,2013,37(04):42.
[2]刘配军,李景富,许向阳,等.番茄hp1和dg基因高效检测体系的建立[J].北方园艺,2013,37(04):99.
[3]王晓娥,李建国,赵姝荣,等.咸阳市设施番茄根结线虫病的发生与防治[J].北方园艺,2013,37(04):133.
[4]谷婧玥,李景富,许向阳,等.番茄不同果穗间品质性状差异性研究[J].北方园艺,2014,38(12):26.
　GU Jing-yue,LI Jing-fu,XU Xiang-yang,et al.Study on Tomato’s Quality and Traits at Different Tassel[J].Northern Horticulture,2014,38(17):26.
[5]吕 涛,于 杰,赵 勇.日光温室越冬栽培番茄适宜品种筛选试验[J].北方园艺,2014,38(12):40.
　LV Tao,YU Jie,ZHAO Yong.Selection of Overwintering Tomato Varieties Suitable for Solar Greenhouse in Heilongjiang[J].Northern Horticulture,2014,38(17):40.
[6]李美芹,裴华丽,乔 宁,等.TYLCV外壳蛋白基因植物表达载体构建及转化番茄的研究[J].北方园艺,2014,38(12):84.
　LI Mei-qin,PEI Hua-li,QIAO Ning,et al.Study on the Construction of Plant Expression Vector of TYLCV-CP Gene and Its Transferring Into Tomato[J].Northern Horticulture,2014,38(17):84.
[7]李文枫,李景富,许向阳.不同hp基因类型番茄中番茄红素与主要品质性状的差异及相关分析[J].北方园艺,2014,38(13):97.
　LI Wen-feng,LI Jing-fu,XU Xiang-yang.Correlation and Difference Analysis Between Lycopene Content and Main Quality Characters of Tomato with Different hp Gene Types[J].Northern Horticulture,2014,38(17):97.
[8]张 浩,李福云,徐志然,等.不同套作模式对温室连作番茄生长产量及土壤微生物与酶活性的影响[J].北方园艺,2014,38(13):46.
　ZHANG Hao,LI Fu-yun,XU Zhi-ran,et al.Effects of Different Relay Intercropping Patterns on Continuous Cropping Tomato Growth，Yield and Soil Microflora and Enzymes Activities in Solar Greenhouse[J].Northern Horticulture,2014,38(17):46.
[9]杨会芳,梁新安,常介田,等.叶面喷施硒肥对不同蔬菜硒富集及产量的影响[J].北方园艺,2014,38(11):158.
　YANG Hui-fang,LIANG Xin-an,CHANG Jie-tian,et al.Effects of Foliar Spraying of Selenium Fertilizer on Selenium Enrichment of Different Vegetables and Yield[J].Northern Horticulture,2014,38(17):158.
[10]祁连弟,管建慧,其日格,等.不同比例牛羊粪便栽培基质对温室番茄苗期生长的影响[J].北方园艺,2013,37(22):47.
　QI Lian-di,GUAN Jian-hui,QI Ri-ge,et al.Effects of Different Nursery Substrates with Cow and Sheep Dung on the Seedlings Growth of Tomato in Solar Greenhouse[J].Northern Horticulture,2013,37(17):47.
[11]贾 　 媛.番 茄 裂 果 的 产 生 与 防 治[J].北方园艺,2001,25(02):0.[doi:10.11937/bfyy.200102021]
　[J].Northern Horticulture,2001,25(17):0.[doi:10.11937/bfyy.200102021]

备注/Memo

第一作者简介：张冬野（1992-），女，博士研究生，研究方向为番茄遗传育种。E-mail：15045662371@163.com.责任作者：许向阳（1969-），男，博士，研究员，博士生导师，研究方向为番茄遗传育种。E-mail：xxy709@126.com.基金项目：国家现代农业产业技术体系专项资金资助项目(CARS-25-A-15)；黑龙江省大宗蔬菜新品种选育资助项目(GA15B103)。收稿日期：2017-04-01

更新日期/Last Update: 2017-09-29