[1]赖黎丽,张嘉园,杨梅,等.新疆加工型辣椒花药培养技术研究[J].北方园艺,2017,41(19):6-10.[doi:10.11937/bfyy.20170971]
　LAI Lili,ZHANG Jiayuan,YANG Mei,et al.Technological Study of Processing Pepper Anther Culture in Xinjiang[J].Northern Horticulture,2017,41(19):6-10.[doi:10.11937/bfyy.20170971]
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新疆加工型辣椒花药培养技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年19 页码: 6-10 栏目: 出版日期: 2017-10-15
Title:
Technological Study of Processing Pepper Anther Culture in Xinjiang
作者:
赖黎丽1; 2; 张嘉园1; 杨梅1; 宋文胜2; 朱华国1
1.石河子大学 农学院，新疆 石河子 832000；2.新疆隆平高科红安种业有限责任公司，新疆 石河子 832000
Author(s):
LAI Lili1; 2; ZHANG Jiayuan1; YANG Mei1; SONG Wensheng2; ZHU Huaguo1
1.College of Agronomy,Shihezi University,Shihezi,Xinjiang 832000；2.Xinjiang Longping High-Tech Hongan Seeds Co.Ltd.，Shihezi,Xinjiang 832000
关键词:
辣椒; 花药培养; 胚状体
Keywords:
pepper; anther culture; embryo
DOI:
10.11937/bfyy.20170971
文献标志码:
A
摘要:
以6个新疆加工型辣椒品种207、208、64、66、67、HT113为试材，以MSB培养基为基本培养基，附加不同植物激素浓度的NAA、2,4-D、TDZ、KT进行花药培养，研究不同植物激素处理对加工型辣椒花药愈伤组织和胚状体诱导的影响，以建立适应于新疆加工型辣椒的花药培养技术。结果表明：不同基因型辣椒的愈伤组织诱导率和出胚率均不同，愈伤组织诱导率为4.98%~29.65%，胚状体诱导率为0~6.88%。0.5 mg?L-1 2,4-D+0.1 mg?L-1 TDZ和 0.5 mg?L-1 NAA+0.1 mg?L-1 KT分别是愈伤组织诱导和胚状体诱导最适宜的激素组合；在新疆特有的气候条件和种植模式下，7月中旬前的花药接种培养最为适宜；胚状体继续发育成熟后，通过练苗移栽获得了56株单倍体植株。
Abstract:
Six processing pepper varieties 207,208,64，66，67 and TH113 in Xinjiang were used for anther culture.The anthers in different stages were induced in basic MSB medium supplemented with NAA,2,4-D,TDZ and KT in various concentrations.Effects of different hormone treatment on processing pepper anther culture were studied,and established a suitable anther culture method for processing pepper in Xinjiang.The results showed that different ratio of callus induction and embryo induction emerged in different genotype peppers,the ratio of callus induction were 4.98%-29.65% and the ratio of embryo induction were 0-6.88%.0.5 mg?L-1 2,4-D+0.1 mg?L-1 TDZ and 0.5 mg?L-1 NAA+0.1 mg?L-1 KT were the suitable hormone combination for callus induction and embryo induction,respectively.Anther culture conducted before middle of July in Xinjiang was effective.In this study,56 haploid plants were achieved by acclimatization and transplant followed embryo development.

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备注/Memo

第一作者简介：赖黎丽（1983-），女，硕士，农艺师，现主要从事园艺植物生理等研究工作。E-mail：58859598@qq.com.

责任作者：朱华国（1979-），男，博士，副教授，现主要从事植物生物技术等研究工作。E-mail：zhhg_agr@shzu.edu.cn.
基金项目：兵团人才发展博士后专项资助项目（CZ0014）。
收稿日期：2017-07-18

更新日期/Last Update: 2017-10-11