HE Zhigang,LOU Chunrong,WANG Xiujuan,et al.Nitrogen and Potassium on Soil Microbial Communities of Tomato and Soil Nutrient and Salinity[J].Northern Horticulture,2017,41(09):148-153.[doi:10.11937/bfyy.201709032]
氮钾配施对设施番茄土壤微生物群落及土壤养分和盐分的影响
- Title:
- Nitrogen and Potassium on Soil Microbial Communities of Tomato and Soil Nutrient and Salinity
- 文献标志码:
- A
- 摘要:
- 为了探明不同氮钾肥用量对番茄根际土壤细菌群落及土壤养分和盐分的影响，以番茄为研究对象，设置不同氮肥和钾肥用量，通过土培盆栽模拟试验,利用高通量测序技术测定根际土壤微生物及土壤养分的变化。结果表明：1）施肥使主要的优势菌群发生明显改变，过量施肥显著降低根际土壤的细菌数量，根际土壤中肠杆菌科（Enterobacteriaceae）细菌的相对丰度明显下降，但鞘脂单胞菌科（Sphingomonadaceae）和黄单胞菌科（Xanthomonadaceae）相对丰度略有增加，细菌种群分布趋于单一化。2）过量施肥导致的土壤电导率(EC)增加、C/N下降，是土壤根际细菌群落发生改变的主要原因。3)过量施肥显著降低番茄株高、茎粗，但叶片叶绿素SPAD相对含量却呈现增加趋势，叶片光合作用受到抑制，从而产量明显下降。研究表明，施肥过量是导致设施土壤次生盐渍化的主要因素，次生盐渍化导致土壤中细菌群落发生改变，植株生长受到抑制，从而产量下降。
- Abstract:
- In order to investigate the influence of different nitrogenous and potassium fertilizers on soil bacterial community and soil nutrient and salt changes in tomato rhizosphere soil.Tomato was used as test object,different dosage of nitrogenous and potassium were set,with indoor pot simulation tests using high-throughput sequencing methods,the change of the soil bacterial community and soil nutrient and salt were studied.The results showed that,1)Fertilization significantly changed the dominant microorganisms,high fertilization significantly reduced the number and species of bacteria in the soil.In the dominant microorganisms Enterobacteriaceae relative abundance significantly reduced，Sphingomonadaceae and Xanthomonadaceae relative abundance slightly increased.The dominant microorganisms tended to be single.2)High fertilization significantly increased the soil conductivity (EC) and decreased the soil C/N，was the main reason for the change of the soil the dominant microorganisms.3)High fertilization significantly decreased the plant height and diameter but significantly increased SPAD.High fertilization led to the inhibition of leaf photosynthesis，resulting in a significant decline in yield.The results showed that high fertilization was the main factor leading to the secondary salinization of the soil，and the secondary salinization led to the change of the bacterial community in the soil,and the secondary salinization led to the inhibition of leaf photosynthesis，resulting in a significant decline in yield.
参考文献/References:
[1]王琦,李锋瑞,张智慧.灌溉与施氮对黑河中游新垦沙地农田土壤硝态氮动态的影响[J].环境科学,2008,29(7):291-299. [2]尹辉,张恩和,王琦,等.春小麦留茬处理对复种油菜产量和水分利用效率的影响[J].农业工程学报,2011,27(2):83-88. [3]CHESHIRE M V,BEDROCK C N,WILLIAMS B L,et al.The immobilization of nitrogen by straw decomposition in soil[J].Eur J Soil Sci,1999,50:320-341. [4]ACKSON L E,WYLAND L J,STRIVERS L J.Winter cover crops to minimize nitrate losses in intensive lettuce production[J].J Agric Sci,1993,121:55-62. [5]王秀娟,袁兴福,娄春荣,等.不同氮钾用量对番茄生长和叶片超微结构的影响[J].中国土壤与肥料,2014(3):44-48. [6]HUSE S M,HUBER J A,MORRISON H G,et al.Accuracy and quality of massively parallel DNA pyrosequencing[J].Genome Biology,2007,8(7):R143. [7]ZHANG Y,WANG X,HU M,et al.Effect of hydraulic retention time(HRT) on the biodegradation of trichloroethylene waste water and anaerobic bacterial community in the UASB reactor[J].Applied Microbiology and Biotechnology,2015,99(4):1977-1987. [8]鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,1999. [9]杨荣,苏永中.水氮配合对绿洲沙地农田玉米产量、土壤硝态氮和氮平衡的影响[J].生态学报,2009,29(3):1459-1469. [10]郑成岩,于振文,王西芝,等.灌水量和时期对高产小麦氮素积累、分配和转运及土壤硝态氮含量的影响[J].植物营养与肥料学报,2009,15(6):1324-1332. [11]沙海宁,孙权,李建设,等.不同施氮量对设施番茄生长与产量的影响及最佳用量[J].西北农业学报,2010,19(3):104-108. [12]陈竹君,王益权,许安民,等.施用不同种类氮肥对日光温室土壤溶液离子组成的影响[J].植物营养与肥料学报,2008,14(5):907-913. [13]SHEN C,XIONG J,ZHANG H,et al.Soil pH drives the spatial distribution of bacterial communities along elevation on Changbai Mountain[J].Soil Biology and Biochemistry,2013,57(3):204-211. [14]赵文艳,张晓敏,石宗琳,等.氮钾肥施用对土壤有效养分和盐分及番茄生长的影响[J].水土保持学报,2011(4):100-103,109.
相似文献/References:
[1]吴晓燕,张丽荣,马建华,等.一种新型微生物制剂对设施番茄土壤微生物数量的影响[J].北方园艺,2013,37(04):42.
[2]张 浩,李福云,徐志然,等.不同套作模式对温室连作番茄生长产量及土壤微生物与酶活性的影响[J].北方园艺,2014,38(13):46.
ZHANG Hao,LI Fu-yun,XU Zhi-ran,et al.Effects of Different Relay Intercropping Patterns on Continuous Cropping Tomato Growth，Yield and Soil Microflora and Enzymes Activities in Solar Greenhouse[J].Northern Horticulture,2014,38(09):46.
[3]朱盼盼,张显,任自力.不同施肥处理对连作西瓜土壤微生物区系的影响[J].北方园艺,2013,37(11):171.
ZHU Pan-pan,ZHANG Xian,REN Zi-li.Effects of Different Fertilizer Application on Soil Microflora of Continuous Cropping Watermelon[J].Northern Horticulture,2013,37(09):171.
[4]雷明,李昌晓,马朋,等.岸坡坡耕地不同耕地类型下土壤酶与土壤微生物的研究[J].北方园艺,2013,37(11):175.
LEI Ming,LI Chang-xiao,MA Peng,et al.Effects of Different Farmland Use Patterns on Soil Enzymes Activities and Soil Microorganisms in Sloped Farmland on Riverbank Slopes[J].Northern Horticulture,2013,37(09):175.
[5]张翠翠,张少伟,常介田.葡萄园行间生草对土壤养分及微生物的影响[J].北方园艺,2013,37(03):181.
ZHANG Cui-cui,ZHANG Shao-wei,CHANG Jie-tian.Effect of Green Covering on Soil Nutrient and Microorganism of Vineyard[J].Northern Horticulture,2013,37(09):181.
[6]穆瑞瑞.土壤微生物在农村植物病害生物防治中的作用分析[J].北方园艺,2013,37(08):194.
MU Rui-rui.Analysis of Soil Microorganism on Biological Control of Plant Diseases in Rural Areas[J].Northern Horticulture,2013,37(09):194.
[7]高亚娟,王永和,杜岩,等.大棚草莓连作对土壤微生物区系和土壤养分含量的影响[J].北方园艺,2013,37(21):56.
GAO Ya-juan,WANG Yong-he,DU Yan,et al.Effect of Strawberry Continuous Cropping on Soil Microflora and Soil Nutrients Content in Greenhouses[J].Northern Horticulture,2013,37(09):56.
[8]马建华,张丽荣,杜玉宁.不同蔬菜种植模式对设施栽培土壤微生物和枯萎病发生的影响[J].北方园艺,2012,36(22):38.
MA Jian-hua,ZHANG Li-rong,DU Yu-ning.Effect of Different Vegetable Cropping Patterns on Soil Microbes and Wilt in Facilities Cultivation[J].Northern Horticulture,2012,36(09):38.
[9]张丽荣,马建华,杜玉宁.不同生物制剂对黄瓜土壤微生物数量及发病率和产量的影响[J].北方园艺,2012,36(21):115.
ZHANG Li-rong,MA Jian-hua,DU Yu-ning.Effect of Different Biological Agents on the Number of Soil Microorganisms,Morbidity and Yield of Cucumber[J].Northern Horticulture,2012,36(09):115.
[10]樊建琼,王延平,韩明玉,等.土壤管理方式对苹果园土壤有机质含量和酶活性及微生物数量的影响[J].北方园艺,2013,37(24):172.
FAN Jian-qiong,WANG Yan-ping,HAN Ming-yu,et al.Effects of Soil Management Ways on Soil Organic Matters Content，Enzyme Activities and Microbes Number of the Apple Orchard[J].Northern Horticulture,2013,37(09):172.
[11]韦鑫,张朝权,谢彩云,等.果园生草对梨园土壤理化性质和微生物的影响[J].北方园艺,2023,(18):79.[doi:10.11937/bfyy.20224599]
WEI Xin,ZHANG Chaoquan,XIE Caiyun,et al.Effects of Grasses Cultivation on Soil Physical and Chemical Properties and Microorganism of Pear Orchard[J].Northern Horticulture,2023,(09):79.[doi:10.11937/bfyy.20224599]
[12]刘莹,孙文松,李旭,等.不同有机水溶肥对人参生长及土壤微生物群落结构的影响[J].北方园艺,2024,(9):78.[doi:10.11937/bfyy.20233780]
LIU Ying,SUN Wensong,LI Xu,et al.Effects of Different Organic Water Soluble Fertilizers on Growth and Soil Microbial Community Structure of Ginseng[J].Northern Horticulture,2024,(09):78.[doi:10.11937/bfyy.20233780]
[13]史芳芳,王海琪,冯娇.轮作十字花科蔬菜对草莓土壤微生态环境的影响[J].北方园艺,2024,(20):63.[doi:10.11937/bfyy.20232775]
SHI Fangfang,WANG Haiqi,FENG Jiao.Effects of Rotating Cruciferous Vegetables on the Microbiotic Environment of Strawberry Soil[J].Northern Horticulture,2024,(09):63.[doi:10.11937/bfyy.20232775]
备注/Memo
第一作者简介：何志刚（1978-），男，硕士，副研究员，现主要从事设施土壤等研究工作。E-mail：hezhigang1227@126.com.责任作者：娄春荣（1966-），男，硕士，研究员，现主要从事设施土壤等研究工作。E-mail：lcrlys@126.com.基金项目：国家现代农业产业技术体系辽宁省科技厅农业攻关资助项目（201415003）；辽宁省科技厅创新人才资助项目（2015028）。