WANG Fei,SONG Bing,LI Dan,et al.Preliminary Screening of Lentinula edodes Mutant Strains by 60Co-γ Irradiation[J].Northern Horticulture,2017,41(15):151-157.[doi:10.11937/bfyy.20170775]
香菇60Co-γ辐照突变菌株的筛选
- Title:
- Preliminary Screening of Lentinula edodes Mutant Strains by 60Co-γ Irradiation
- Keywords:
- Lentinula edodes; 60Co-γ; radiation mutagenesis; cellulose; hemicellulose
- 文献标志码:
- A
- 摘要:
- 以香菇栽培菌株“庆20”作为诱变材料，分别利用0.4、0.6、0.8 kGy 3种辐射剂量，剂量率为10 Gy?min-1的60Co-γ射线对香菇菌株进行物理诱变，以不辐射处理为对照，研究了不同辐射剂量对香菇菌株诱变率和致死率的影响，将诱变菌株与对照菌株进行拮抗试验和SSR分子鉴定，并对突变菌株对纤维素和半纤维素的利用能力进行筛选，以期筛选出能更好的利用秸秆作为栽培基质的香菇栽培菌株。结果表明：辐射剂量为0.4 kGy 时，所有菌株致死率为0，0.6 kGy 时致死率为 46.7 %,0.8 kGy 时致死率达到 100 %。经过拮抗试验和SSR分子标记的筛选，得到6株符合要求突变菌株。
- Abstract:
- The Lentinus edodes strain ‘Qing 20’ was irradiated by 60Co-γ ray,and the mutagen dosage was respectively 0.4 kGy,0.6 kGy,0.8 kGy,dose rate was 10 Gy?min-1.Non-radiating treatment was CK.The effect of different mutagen dosages on the lethality rate of Lentinus edodes was studied.The mutant strains were identified by antagonistic reaction and SSR molecular marker,and utilization ratio of cellulose and hemicellulose was preliminarily screened.The results showed that all strains were alive after treated with 0.4 kGy,the death rate was 46.7% after treated with 0.6 kGy,and the death rate was 100% after treated with 0.8 kGy.Six mutant strains were screened out by antagonistic reaction and SSR,and the utilization ratio of cellulose and hemicellulose were better than CK.
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备注/Memo
第一作者简介：王菲（1990-），女，硕士研究生，研究方向为食用菌栽培基质。E-mail：13944800667@sina.cn.责任作者：李玉（1944-），男，博士，教授，中国工程院院士，研究方向为菌物学。E-mail:fungi966@126.com.基金项目：公益性行业(农业)科研专项资助项目(201503137)；国家重点基础研究发展规划资助项目(973 计划) (2014CB138305)；吉林省秸秆综合利用技术创新平台资助项目(吉高平合字2014B-1)；吉林农业大学校内启动基金资助项目(2015007)。