(College of Agriculture,Guizhou University/Guizhou Fruits Engineering Technology Research Centre,Guiyang,et al.Differences of Phosphorus Efficiency Characteristics and Root Properties Between Two Citrus Seedlings Under Phosphorus Stress[J].Northern Horticulture,2017,41(15):12-17.[doi:10.11937/bfyy.20170693]
低磷胁迫下两种柑橘砧木的磷效率特征及其根系性状差异
- Title:
- Differences of Phosphorus Efficiency Characteristics and Root Properties Between Two Citrus Seedlings Under Phosphorus Stress
- Keywords:
- citrus; phosphorus efficiency; root; free amino acid
- 文献标志码:
- A
- 摘要:
- 以土橘（Citrus chuana Hort.）和枳（Poncirus trifoliata Raf.）为试材，在砂培条件下，研究不同供磷条件下其生物量、磷素积累量、根系性状的差异，从根系性状差异的角度阐述2种柑橘对低磷胁迫的响应特征及适应机理，为找出影响柑橘磷素(P)吸收的主要因子和通过根系塑性提高养分利用效率的遗传改良提供参考依据。结果表明：枳砧木磷效率为91.22%，土橘为53.24%。低P胁迫下枳砧木P吸收效率显著降低（降幅70%)，P转移效率、P利用效率显著提高(增幅分别为59%、300%)；土橘P吸收效率、P转移效率显著降低（降幅分别为87%、6%)，P利用效率显著提高(增幅200%)。土橘根冠比在低P胁迫下显著增加，增幅为120%，而枳根冠比却显著降低，降幅为24%。低P胁迫下2种柑橘砧木的根系总长度、总表面积、体积、平均直径、根系游离氨基酸总量、脯氨酸和羟脯氨酸含量都显著降低。试验表明，相较而言枳为P高效柑橘种质，土橘为P低效种质。增大根冠比，降低根系总长度、总表面积、体积、根尖数、根系游离氨基酸总量和脯氨酸含量以降低P吸收、转移效率，提高P利用效率是柑橘种质适应低磷环境的突出表现，这在P低效种质土橘中表现尤其明显。而P高效种质枳在低磷环境中却能保持正常的根尖数，降低根冠比并提高P转移效率，同时羟脯氨酸含量与P转移效率呈显著负相关。
- Abstract:
- Two citrus seedlings (Citrus chuana Hort.and Poncirus trifoliata Raf.) were used as test materials，and evaluated for differences in dry matter weight，phosphorus accumulation and root properties under different phosphorus (P) application rates by using a sand culture method.Efficient genotype citrus played an important roles in promoting phosphorus stress from the perspective of the root properties.The purpose of this study was to provide a scientific basis for identifying the main factors that affecting phosphorus absorption of citrus and improving efficiency of nutrient utilization of genetic improvement through root plasticity.The results showed that the P efficiency of P.trifoliata was 91.22%，and C.chuana 53.24%.Under low P stress the P absorption efficiency of P.trifoliata significantly was reduced by 70%，P transfer and utilization efficiency significantly got an increase of 59% and 300%；meanwhile the P absorption and transfer efficiency of C.chuana were significantly reduced by 87% and 6%，P utilization efficiency increased 200%.The root/shoot ratio of C.chuana was significantly higher than in the sand with low P stress，with an increase of 120%，while the P.trifoliata was significantly decreased，with a decline of 24%.With low P stress，the total length of root，total surface area，average diameter，root volume，total free amino acid，proline and hydroxyproline content of 2 kinds of citrus seedlings were significantly decreased.In contrast，P.trifoliata was classified as efficient P type，while C.chuana inefficient P type.Outstanding performances of citrus germplasm adaptation to low phosphorus environment were increasing the ratio of root to shoot，decreasing the total length of root，total surface area，root volume，tips，total free amino acid and praline content to reduce P absorption，transfer efficiency and improve the efficiency of P utilization.This performance was particularly evident in the inefficient P type C.chuana.But the efficient P type P.trifoliata in low phosphorus environments could maintain normal tips，reduce root/shoot ratio and improve the P transfer efficiency.The content of hydroxyproline and P transfer efficiency were negatively correlated.
参考文献/References:
? [1]龙健,江新荣,邓启琼,等.贵州喀斯特地区土壤石漠化的本质特征研究[J].土壤学报,2005,42(3)：419-427. [2]YANG L T，JIANG H X，QI Y P，et al.Differential expression of genes involved in alternative glycolytic pathways，phosphorus scavenging and recycling in response to aluminum and phosphorus interactions in Citrus roots[J].Mol Biol Rep,2012,39:6353-6366. [3]罗燕,樊卫国.不同施磷水平下4种柑橘砧木的根际土壤有机酸、微生物及酶活性[J].中国农业科学,2014,47(5):955-967. [4]樊卫国,罗燕.不同磷水平下4种柑橘砧木的生长状况、根系形态和生理特性[J].中国农业科学,2015,48(3):534-545. [5]樊卫国,王立新.供磷水平对纽荷尔脐橙幼树磷酸酶活性和磷素吸收利用效率的影响[J].西南农业学报,2013,26(1):217-222. [6]先宗良,邓大林,兰庆渝.柑桔砧木对柑桔脚腐病菌(Phytophthora parasitica)的抗性研究[J].四川果树科技,1990,18(2):29-30. [7]马文涛,樊卫国.不同种类柑橘的抗旱性及其与内源激素变化的关系[J].应用生态学报,2014，25(1):147-154. [8]陈波浪,盛建东,蒋平安,等.不同基因型棉花磷效率特征及其根系形态的差异[J].棉花学报,2014,26(6):506-512. [9]梁翠月,廖红.植物根系响应低磷胁迫的机理研究[J].生命科学,2015(3):389-397. [10]罗佳,候银莹,程军回,等.低磷胁迫下不同磷效率基因型棉花的根系形态特征[J].中国农业科学,2016,49(12):2280-2289. [11]申建波,毛达如.植物营养研究方法[M].北京:中国农业大学出版社,2011:12-20. [12]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000. [13]鲁敏,安华明,赵小红.无籽刺梨与刺梨果实中氨基酸分析[J].食品科学,2015,36(14):118-121. [14]马振强,臧兴隆,孙鲁龙,等.几种葡萄砧木及其与赤霞珠嫁接组合磷效率比较[J].园艺学报，2014(7):1289-1296. [15]DUNCAN R R.Genetic manipulation.Plant-environment interactions[M].New York：Marcel Dekker，Inc:1994:1-39. [16]杨青,张一,周志春,等.低磷胁迫下不同种源马尾松的根构型与磷效率[J].应用生态学报，2012,23(9):2339-2345. [17]WU P F，MA X Q，TIGABU M，et al.Root morphological plasticity and biomass production of two Chinese fir clones with high phosphorus efficiency under low phosphorus stress[J].Canadia Journal of Forest Research,2011,41(2):228-234. [18]郭玉春,林文雄,石秋梅,等.低磷胁迫下不同磷效率水稻苗期根系的生理适应性研究[J].应用生态学报,2003,14(1):61-65. [19]林雅茹,唐宏亮,申建波.野生大豆根系形态对局部磷供应的响应及其对磷吸收的贡献[J].植物营养与肥料学报,2013,19(1):158-165. [20]曹秀,夏仁学,杨环宇,等.沙培条件下磷、钾、钙亏缺对枳(Poncirus trifoliata)幼苗根系形态及营养吸收的影响[J].植物营养与肥料学报,2014(4):981-988. [21]季萌萌,许海港,彭玲,等.低磷胁迫下五种苹果砧木的磷吸收与利用特性[J].植物营养与肥料学报,2014(4):974-980. [22]季萌萌,彭玲,文炤,等.供磷水平对苹果砧木氮、磷吸收和利用特性的影响[J].植物营养与肥料学报,2015,21(4):1016-1021. [23]ASHRAF M，HARRIS P J C.Potential biochemical indicators of salinity tolerance in plants[J].Plant Science,2004,166(1):3-16. [24]谭勇,梁宗锁,王渭玲,等.氮、磷、钾营养对膜荚黄芪幼苗根系活力和游离氨基酸含量的影响[J].西北植物学报,2006,26(3):478-483.
相似文献/References:
[1]李松丽,黄欢欢,蔡小东.两种凝固剂对冰糖橙愈伤组织增殖及植株再生的影响[J].北方园艺,2013,37(22):108.
LI Song-li,HUANG Huan-huan,CAI Xiao-dong.Effects of Two Types of Gelling Agents on the Callus Proliferation and Plant Regeneration of Citrus sinensis L.Osbeck cv.Bingtang[J].Northern Horticulture,2013,37(15):108.
[2]陈志远,邸丽俊,王国栋,等.柑橘冻害抵抗分子机理研究进展[J].北方园艺,2014,38(01):188.
CHEN Zhi-yuan,DI Li-jun,WANG Guo-dong,et al.Review of Molecular Mechanism of Cold Resistance in Citrus[J].Northern Horticulture,2014,38(15):188.
[3]周梦娇,万春鹏,陈金印.植物提取物对柑橘采后病害抑制及保鲜效果的研究进展[J].北方园艺,2014,38(02):186.
ZHOU Meng-jiao,WAN Chun-peng,CHEN Jin-yin.Research Progress on Inhibition of Citrus Postharvest Diseases and Preservation Effects of Plant Extraction[J].Northern Horticulture,2014,38(15):186.
[4]胡选萍.不同生长调节因子对耐寒柑橘愈伤组织诱导效应研究[J].北方园艺,2014,38(09):109.
HU Xuan-ping.Study on Effect of Different Growth-regulators on Callus Induction of Cotyledon Explants About Critus rutaceae [J].Northern Horticulture,2014,38(15):109.
[5]李云明,顾云琴,项顺尧,等.柑橘小实蝇对柑橘产量的影响[J].北方园艺,2012,36(09):133.
LI Yun-ming,GU Yun-qin,XIANG Shun-yao,et al.Effect of Bactrocera dorsalis to the Production of Citrus[J].Northern Horticulture,2012,36(15):133.
[6]马杰,刘今朝,刘涓,等.三峡库区水稻田改种柑橘的立地条件及其改造技术研究[J].北方园艺,2014,38(17):163.
MA Jie,LIU Jin-zhao,LIU Juan,et al.Study on Site Conditions and Engineering Design of Citrus Orchard’s Planting Field in Paddy Field in Three Gorges Area[J].Northern Horticulture,2014,38(15):163.
[7]安 冬 梅,孙 爱 红,孟 长 军.柑橘果酒加工工艺初探[J].北方园艺,2010,34(04):0.[doi:10.11937/bfyy.201004065]
AN Dong - mei,SUN Ai - hong,MENG Chang - jun.Study on the Processing T echnology of CitrousFermented Wine[J].Northern Horticulture,2010,34(15):0.[doi:10.11937/bfyy.201004065]
[8]刘 莉１,刘 霞２,包 永 华 １.碳酸钠、 碳酸氢钠和山梨酸钾对柑橘采后酸 腐 病 菌 的 抑 制 作 用[J].北方园艺,2011,35(23):0.[doi:10.11937/bfyy.2011023052]
,Ｉ ｎ ｈ ｉ ｂ ｉ ｔ ｉ ｏ ｎ Ｅ ｆ ｆ ｅ ｃ ｔ ｓ ｏ ｎ Ｓ ｏ ｕ ｒ Ｒ ｏ ｔ ｏ ｆ Ｃ ｉ ｔ ｒ ｕ ｓ Ｆ ｒ ｕ ｉ ｔ ｓ ｂ ｙ Ａ ｐ ｐ ｌ ｉ ｃ ａ ｔ ｉ ｏ ｎ ｏ ｆＳ ｏ ｄ ｉ ｕ ｍ Ｃ ａ ｒ ｂ ｏ ｎ ａ ｔ ｅ ， Ｓ ｏ ｄ ｉ ｕ ｍ Ｂ ｉ ｃ ａ ｒ ｂ ｏ ｎ ａ ｔ ｅ ａ ｎ ｄ Ｐ ｏ ｔ ａ ｓ ｓ ｉ ｕ ｍ Ｓ ｏ ｒ ｂ ａ ｔ ｅ[J].Northern Horticulture,2011,35(15):0.[doi:10.11937/bfyy.2011023052]
备注/Memo
作者简介：鲁敏（1984-），女，四川乐山人，博士，讲师，现主要从事果树生理与分子生物学等研究工作。E-mail：48181266@qq.com.基金项目：贵州大学引进人才科研资助项目 （贵大人基合字[2011]07号）。 收稿日期：2017-04-11