GUO Xiao,YUAN Guangxin,WANG Manli,et al.Bioinformatics Analysis of Solanum lycopersicum Superoxide Dismutase[J].Northern Horticulture,2016,40(21):97-101.[doi:10.11937/bfyy.201621025]
番茄超氧化物歧化酶的生物信息学分析
- Title:
- Bioinformatics Analysis of Solanum lycopersicum Superoxide Dismutase
- 文献标志码:
- A
- 摘要:
- 超氧化物歧化酶（SOD）是生物体内重要的抗氧化酶。以在NCBI数据库中注册的7种番茄SOD蛋白质为分析对象，利用生物信息学方法，分析了SOD蛋白质的理化性质、信号肽、糖基化位点、磷酸化修饰、一级和二级结构以及系统进化特点。结果表明：7种蛋白质间的理化性质存在较大差异；氨基酸序列中不含信号肽，均不是分泌蛋白；不同类型的番茄SOD中所含糖基化以及潜在磷酸化位点数目不同；它们的氨基酸序列同源性较低，二级结构也存在较大差异；系统进化树可作为番茄SOD家族蛋白遗传分化研究的重要依据。该研究为深入开展SOD的酶学特征及植物抗氧化的分子机制研究提供重要的理论依据。
- Abstract:
- Superoxide dismutase (SOD) is an important antioxidant enzyme in organisms.Physicochemical properties，signal peptide，glycosylation sites，phosphorylated modification，primary and secondary structures and molecular phylogenetic evolution of seven Solanum lycopersicum SOD registered in NCBI had been analyzed in this study.The results showed that there were differences in their physicochemical properties.As there was no signal peptide on their amino acid sequences，none of them was secreted protein.The numbers of glycosylation sites and phosphorylated sites were different.Sequence alignment revealed that they had low homologous in different kinds of Solanum lycopersicum SOD，as well as the secondary structures.Molecular evolution could be as an important basis for the genetic differentiation and molecular evolution of Solanum lycopersicum SOD.This study provided a theoretical basis for both the enzymatic mechanism of SOD and molecular mechanism of plant resistance.
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备注/Memo
第一作者简介：郭笑（1988-），女，博士，实验师，现主要从事基因工程产品制备等研究工作。E-mail:liguoxiao2697@126.com.责任作者：安丽萍(1973-)，女，博士，副教授，硕士生导师，现主要从事生物信息学分析与蛋白质纯化及结构等研究工作。E-mail:alp960@126.com.基金项目：长白山药用动植物活性多肽研究与开发国家地方联合工程实验室资助项目（发改高技[2010]2455号）；吉林省教育厅资助项目 (吉教科合[2016]第75号)。