DU Shanshan,LI Siyi,WANG Dong,et al.Establishment of Regeneration System for Cucumis melo L.cv.‘Jiashi’ of Xinjiang Melon[J].Northern Horticulture,2016,40(16):89-92.[doi:10.11937/bfyy.201616024]
新疆甜瓜“伽师”再生体系的建立
- Title:
- Establishment of Regeneration System for Cucumis melo L.cv.‘Jiashi’ of Xinjiang Melon
- 关键词:
- 甜瓜; 6-苄氨基嘌呤（6-BA）; 硝酸银（AgNO3）; 再生体系
- Keywords:
- melon; 6-BA; AgNO3; regeneration system
- 文献标志码:
- A
- 摘要:
- 以厚皮甜瓜“伽师”为试材，研究了6-苄氨基嘌呤（6-BA）、硝酸银（AgNO3）对诱导不定芽的影响，建立以子叶为外植体的快速高效再生体系。选取5 d的“伽师”无菌苗子叶，在添加了不同浓度6-BA的MSB培养基中诱导不定芽；并且探讨了在最适6-BA浓度的培养基中添加不同浓度AgNO3对诱导不定芽的影响。结果表明：甜瓜子叶外植体接种在MSB+1.5 mg?L-1 6-BA诱导分化培养基上，诱导率可达75%；添加不同浓度AgNO3诱导不定芽，以MSB+1.5 mg?L-1 6-BA+2.0 mg?L-1 AgNO3最适，诱导率可达65%，产生单芽较多，缩短了再生周期。丛生芽或单生不定芽转至MSB+0.1 mg?L-1 6-BA+0.1 mg?L-1 GA3的培养基中伸长，不定芽均可伸长且生长状况良好；再将单芽转至MSB+0.01 mg?L-1 NAA培养基进行生根。
- Abstract:
- A high efficiency and rapid regeneration system of thick-skinned muskmelon had been set up using cultivars ‘Jiashi’ cultured in the media with different hormone of 6-Benzolaminopurine(6-BA) and silver nitrate(AgNO3).The 5 days sterile cotyledon of ‘Jiashi’ were cultured in media with different 6-BA concentration.The effect of different AgNO3 concentration added in media with the most appropriate 6-BA concentration was discussed.The results showed that the MSB medium containing 1.5 mg?L-1 6-BA was the most suitable for inducing adventitious buds with an average rate of 75%.The MSB medium containing 1.5 mg?L-1 6-BA+2.0 mg?L-1 AgNO3 was the best suitable for inducing adventitious buds with an average rate of 65%，there were most of single adventitious buds，this combination could save time of regeneration system.Adventitious buds were able to elongation on MSB medium with 0.1 mg?L-1 6-BA and 0.1 mg?L-1 GA3，adventitious buds elongated and grew well.Regenerated shoots were rooted on MSB medium with 0.01 mg?L-1 NAA.
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备注/Memo
第一作者简介：杜姗姗（1991-），女，硕士研究生，研究方向为植物学。E-mail：915037756@qq.com.责任作者：李冠（1949-），男，教授，博士生导师，研究方向为植物生理生化与分子生物学。E-mail：guanli@xju.edu.cn.基金项目：国家自然科学基金资助项目（31260258）。