ZHANG Shuihua,JIANG Yonggang,ZHAO Hongyan.Microwave-assisted Extraction and Antioxidant Activity of Polysaccharides From Lactarius volemus Fr.[J].Northern Horticulture,2016,40(14):135-138.[doi:10.11937/bfyy.201614034]
多汁乳菇多糖的微波辅助提取工艺及其清除DPPH自由基研究
- Title:
- Microwave-assisted Extraction and Antioxidant Activity of Polysaccharides From Lactarius volemus Fr.
- 关键词:
- 多汁乳菇; 多糖; 微波; DPPH自由基清除
- 文献标志码:
- A
- 摘要:
- 以多汁乳菇为试材，研究了液料比、微波功率、提取时间、提取次数对多糖提取率的影响，并采用正交实验优化了工艺条件，对提取产物进行了DPPH自由基清除研究。结果表明：对多糖提取率影响最大是微波功率，其次是液料比，提取时间影响最小；优化工艺条件为液料比20∶1 mL?g-1，微波功率480 W，提取时间4 min，提取3次。在此条件下多糖提取率达6.41%。多糖对DPPH自由基有一定的清除作用，证明多汁乳菇多糖具有一定的抗氧化活性。
- Abstract:
- Taking Lactarius volemus Fr.as the raw material，the effects of extraction conditions such as ratio of liquid-solid，microwave time，microwave power and time of extraction were investigated by one-factor tests and orthogonal test，and the extraction polysaccharides analyzed with DPPH? test.The results showed that the microwave power affected the yield markably，the second was ratio of liquid-solid，and microwave time had the least influence；the optimum coditions were determined as follows：ratio of liquid-solid of 20∶1 mL?g-1，microwave power of 480 W，microwave time of 4 min and times of extraction of 3 times.Under the condition，the yield was up to 6.41%.Polysaccharides exhibited antioxidant effects in 2,2′-diphenyl-1-picrylhydrazyl(DPPH?) radical scavenging assay.And it showed that the extraction polysaccharides which had some oxidation resistance.
参考文献/References:
[1]朱培欣,梅余霞,梁运祥.一种虫草多糖的体外免疫学活性[J].食品科技,2013,38(9):25-28.
[2]王君巧,聂少平,余强,等.黑灵芝多糖对免疫抑制小鼠的免疫调节和抗氧化作用[J].食品科学,2012,33(23):274-277.
[3]NIE S P，ZHANG H，LI W J,et al.Currentdevelopment of polysaccharides from Ganoderma：Isolation,structure and bioactivities[J].Bioactive Carbohydrates and Dietary Fibre，2013,1(1):10-20.
[4]ZHU L N，LUO X，TANG Q J,et al.Isolation，purification，and immunological activities of alow-molecular-weight polysaccharide from the lingzhi or reishi medicinal mushroom Ganoderma lucidum (higher basidiomycetes)[J].International Journal of Medicinal Mushrooms,2013,15(4):407-414.
[5]刘佳,高敏,殷忠.贵州野生多汁乳菇氨基酸成分及致小鼠突变性实验[J].预防医学情报杂志，2007,23(2):230-232.
[6]AHMET C，ZLEM F，ERTU R S.Nutritional composition of some wild edible murshrooms[J].Urkish Journal of Biochemistry，2009,34(1):25-31.
[7]胡先运,王传明,江家志,等.多汁乳菇的研究及应用[J].北方园艺,2014(18):157-160.
[8]王心诗,邰丽梅,吴素蕊,等.响应面法优化水提铜色牛肝菌多糖工艺[J].中国食用菌，2015,34(1):60-64.
[9]张斌,刘莹,王益,等.金针菇菌渣中多糖的微波辅助提取工艺及其抗氧化活性研究[J].天然产物研究与开发，2015,27(1):120-125.
[10]陈景藻.现代物理治疗学[M].北京:人民军医出版社,2001:186-195.
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备注/Memo
第一作者简介：张水花（1980-），女，硕士，副教授,研究方向为天然产物化学及保健食品。E-mail：z_shh@163.com.基金项目：云南省科技厅研究基金资助项目（2013FD047）。