[1]张颖,杨舸.微波提取法优选海州常山叶总黄酮的提取工艺[J].北方园艺,2016,40(10):118-122.[doi:10.11937/bfyy.201610030]
　ZHANG Ying,YANG Ge.Extraction Process Optimization of Total Flavoniod From Clerodendron trichotomum Leaves by Microwave Technology[J].Northern Horticulture,2016,40(10):118-122.[doi:10.11937/bfyy.201610030]
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微波提取法优选海州常山叶总黄酮的提取工艺

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第40卷 期数: 2016年10 页码: 118-122 栏目: 出版日期: 2016-05-30
Title:
Extraction Process Optimization of Total Flavoniod From Clerodendron trichotomum Leaves by Microwave Technology
作者:
张颖1; 2; 杨舸3
（1.攀枝花学院 生物与化学工程学院,四川 攀枝花 617000；2.干热河谷特色生物资源研发四川省高校重点实验室，四川 攀枝花 617000；3.攀枝花市食品与药品检验所，四川 攀枝花 617000）
Author(s):
ZHANG Ying1; 2; YANG Ge3
(1.College of Biological and Chemistry Engineering，Panzhihua University，Panzhihua，Sichuan 617000；2.Key Laboratory of Dry-hot Valley Characteristic Bio-Resources Development at University of Sichuan Province，Panzhihua,Sichuan 617000;3.Panzhihua Institution for Food and Drug Control，Panzhihua,Sichuan 617000)
关键词:
海州常山; 黄酮; 正交实验; 提取
Keywords:
Clerodendron trichotomum; flavoniod; orthogonal design; extraction
DOI:
10.11937/bfyy.201610030
文献标志码:
A
摘要:
以海州常山干叶粉末为试材，采用微波技术提取总黄酮粗品，并通过紫外-可见分光光度计测定样品液黄酮含量，以总黄酮提取量为考察指标，研究了料液比、乙醇浓度、提取时间和提取温度等工艺条件对提效的影响，设计了4因素3水平正交实验，进一步对提取海州常山叶总黄酮的工艺条件进行优化。结果表明：提取海州常山叶总黄酮较佳的提取条件为预浸泡2 h，微波功率200 W，提取温度60 ℃，乙醇溶液浓度70%，料液比1∶35 g?mL-1，微波提取2次，每次3 min，提取总黄酮量达71.15 mg?g-1；微波提取技术应用于海州常山叶总黄酮的提取工艺可行，该技术可为海州常山植物资源的开发和利用提供参考依据。
Abstract:
Taking Clerodendron trichotomum leaves as material,using microwave technology to extract total flavoniod，the flavoniod contents were evaluated by ultraviolet and visible spectrophotometer.The effect of material-liquid ratios，ethanol concentration，extraction temperatures and extraction time were investigated using the yields of total flavoniod as index.The optimum extraction process conditions of total flavoniod were studied by the orthogonal design after single factor experiments.The results showed that the optimum extraction conditions were as follows：presoaking for 2 hours，microwave power 200 W，ethanol concentration 70%，temperature 60 ℃，material-liquid ratio 1∶35 g?mL-1，extracting by 2 times，and each time for 3 minutes，the yield of total flavoniod could reach 71.15 mg?g-1.Microwave technology was feasible for flavoniod extraction，and it also could lay a foundation for further utilization of Clerodendron trichotomum.

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备注/Memo

第一作者简介：张颖（1981-），女，硕士，讲师，现主要从事生物分离与细胞工程的教学与研究等工作。E-mail:zemn@sina.com. 基金项目：攀枝花学院校级资助项目 (2014YB34）。

更新日期/Last Update: 2016-07-11