YANG Pingfei,WU Mingkai,SONG Zhiqin,et al.Effect of Medium of Different Hormone on Dendrobium officinale Buds Proliferation[J].Northern Horticulture,2016,40(06):106-108.[doi:10.11937/bfyy.201606026]
不同激素配比对铁皮石斛组培丛生芽增殖的影响
- Title:
- Effect of Medium of Different Hormone on Dendrobium officinale Buds Proliferation
- Keywords:
- Dendrobium officinale; buds; proliferation culture
- 文献标志码:
- A
- 摘要:
- 以铁皮石斛为试材，以MS为基本培养基，研究了不同激素配比对铁皮石斛组培丛生芽增殖的影响。结果表明：铁皮石斛丛生芽增殖的培养条件为MS+6-BA 1.0 mg/L+ NAA 0.5 mg/L+土豆汁100 g/L+蔗糖30 g/L，90 d增殖系数达7.00，丛生芽芽高、茎径、含水量和叶绿素含量均高于其它处理。
- Abstract:
- Taking Dendrobium officinale as material，MS as basic media，the effect of different hormone ratio on bud proliferation of Dendrobium officinale was studied.The results showed that the most suitable medium of Dendrobium officinale buds proliferation was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+potatoes 100 g/L+sucrose 30 g/L，the proliferation coefficient was 7.00 after 90 days；bud height，stems diameter，moisture content and chlorophyll content were higher than other treatments.
参考文献/References:
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备注/Memo
第一作者简介：杨平飞（1988-），男，贵州长顺人，硕士，研究实习员，现主要从事中药材栽培生理等研究工作。E-mail:505092100.my @163.com.责任作者：吴明开（1970-），男，安徽枞阳人，博士，研究员，现主要从事中药材资源多样性保护和利用及育种与栽培等研究工作。 E-mail:bywmk1999@163.com.基金项目：“贵州珍稀药材石斛、白及品种选育及其产业化”资助项目（黔农科院自主创新科研专项字（2014）017号）；贵州省农业委员会资助项目（GZCYTX2014-0202）；贵州省中药现代化科技专项资助项目（黔科合中药字[2013]5033号）。