CUI Jinxin,LI Yuemei,ZHANG Ruijing,et al.Measuring the Ploidy of Hawthorn Germplasm Resources by Flow Cytometry[J].Northern Horticulture,2016,40(01):84-86.[doi:10.11937/bfyy.201601022]
利用流式细胞仪鉴定山楂种质资源的倍性
- Title:
- Measuring the Ploidy of Hawthorn Germplasm Resources by Flow Cytometry
- Keywords:
- hawthorn; ploidy; flow cytometry; germplasm resources
- 文献标志码:
- A
- 摘要:
- 以25份山楂资源为试材，采用流式细胞仪方法对山楂资源的倍性进行了鉴定，并与前人用幼叶去壁低渗染色体压片法的鉴定结果进行了比较，以期为山楂资源的育种利用提供参考。结果表明：14份材料的倍性与前人的鉴定结果一致，有6份材料与前人的鉴定结果不同，还有5份材料的倍性为首次报道；流式细胞仪法是鉴定山楂种质资源倍性的有效方法，并且该次倍性鉴定结果对今后的山楂杂交育种和种质创新具有一定的参考意义。
- Abstract:
- In order to provide reference for utilization in breeding the ploidy of 25 accessions of hawthorn germplasm resources was measured by flow cytometry and comparison was drawn with others previous measurement by young leaf squash and chromosomes counting method.The results showed that the ploidy of 14 accessions was identical with previous measurements，the ploidy of 6 accessions was different from previous results，and the ploidy of 5 accessions was reported for the first time.This research showed that flow cytometry was a high efficient method for ploidy measurement of hawthorn germplasm resources，and ploidy measurement in this research provided reference for the proper utilization of the germplasm resources in future hawthorn improvement and cross breeding.
参考文献/References:
[1]辛孝贵,张育明.中国山楂种质资源与利用[M].北京:中国农业出版社,1997. [2]张育明,辛孝贵,王巨.中国山楂属植物染色体数目和同工酶的研究[J].中国农业科学,1983(3):37-44. [3]宋文芹,李秀兰,陈端阳,等.我国部分山楂属植物染色体数目的研究[J].园艺学报,1985,12(2):73-76. [4]蒲富慎,林盛华,张德学.我国山楂一些种和品种的染色体数目观察[J].中国果树,1987(2):17-19. [5]辛孝贵.我国山楂属和山楂栽培品种染色体数目的研究[J].沈阳农业大学学报,1991,22(1):27-35. [6]郭太君,丰宝田,孙宪忠.我国北方染色体数目研究[J].特产研究,1989(3):14-15. [7]李斌,石荫坪,束怀瑞,等.利用流式细胞光度术鉴定苹果倍性的研究[J].西北植物学报,1998,18(4):499-504. [8]李斌,束怀瑞,石荫坪,等.流式细胞光度术用于草莓倍性鉴定的研究[J].西北农业大学学报,1998,26(4):45-48. [9]张俊娥,刘继红,邓秀新.采用倍性分析仪鉴定柑橘愈伤组织的遗传变异[J].遗传学报,2003,30(2):169-174. [10]蒋洪恩,刘孟军.秋水仙碱诱导枣多倍体的研究[J].园艺学报,2004,31(5):647-650. [11]陈绪中,罗正荣.鄂柿1号离体叶片秋水仙素诱导和再生植株倍性鉴定[J].果树学报,2005,22(5):554-556. [12]孙马,王跃进.中国野生葡萄染色体倍性研究[J].西北农业学报,2006,15(6):148-152. [13]饶静云,刘义飞,黄宏文.中华猕猴桃不同倍性间杂交后代倍性分离和遗传变异分析[J].园艺学报,2012,39(8):1447-1456. [14]TALENT N,DICKINSON T A.Polyploidy in Crataegus and Mespilus (Rosaceae，Maloideae):evolutionary inferences from flow cytometry of nuclear DNA amounts[J].Can J Bot,2005,83(10):1268-1304. [15]赵焕谆,丰宝田.中国果树志·山楂卷[M].北京:中国林业出版社,1996. [16]丰宝田,郭太君,王铭,等.伏山楂花粉败育和赤霉素刺激单性结实的试验研究[J].特产科学试验,1986,2(2):11-14. [17]MUNIYAMMA M,PHIPPS J B.Cytological proof of apomixis in crataegus (Rosaceae) [J].Amer J Bot,1979,66(2):149-155.
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备注/Memo
第一作者简介：崔金鑫（1989-），女，硕士研究生，研究方向为果树遗传育种。责任作者：张吉军（1972-），男，博士，副教授，研究方向为果树遗传育种。E-mail：zjjqhd@163.com.基金项目：河北科技师范学院博士基金资助项目（2013YB019）。