LU Juan,CHANG Qingquan,WANG Siqi,et al.Optimization of Ultrasonic-assisted Extraction of Juglans regia L. Husk Crude Polysaccharide Using Response Surface Methodology[J].Northern Horticulture,2015,39(22):125-129.[doi:10.11937/bfyy.201522033]
响应面法优化核桃青皮粗多糖超声提取工艺研究
- Title:
- Optimization of Ultrasonic-assisted Extraction of Juglans regia L. Husk Crude Polysaccharide Using Response Surface Methodology
- Keywords:
- Walnut green seedcase; polysaccharide; ultrasonic-assisted extraction; response surface methodology
- 文献标志码:
- A
- 摘要:
- 以核桃青皮为试材，以核桃青皮粗多糖得率为响应值，在单因素试验的基础上，采用3因素3水平响应面分析法，研究超声提取因素对核桃青皮粗多糖得率的影响，确定核桃青皮粗多糖超声提取工艺。结果表明：核桃青皮超声最佳提取条件为超声功率比60%、提取时间30 min、温度60℃，在该条件下核桃青皮粗多糖的提取得率为15.09%。
- Abstract:
- Using walnut green husk as test material，taking the yield of walnut green husk polysaccharide as the response value，on the basis of single factor experiment，the effect of ultrasonic factors on crude polysaccharide from walnut green husk were designed by the response surface methodology with three factors and three levels to determined the optimum technology.The experimental results showed that walnut green husk ultrasonic power radio was 60%，the extraction time was 30 min and the extraction temperature was 60℃.Under the condition the yield of the crude polysaccharide from walnut green husk was 15.09%.
参考文献/References:
[1]江苏新医学院.中药大辞典[M].上海:上海科学技术出版社,1986. [2]张婷,张虹.山核桃青皮化学成分及生物活性研究进展[J].食品科技,2007(5):117-119. [3]李玉彬,马宏图,杨波,等.青龙衣不同提取部位的抗肿瘤作用研究[J].中草药,2004,31(10):1145-1147. [4]周媛媛,王栋.青龙衣化学成分的研究[J].中医药信息,2010,27(2):18-20. [5]刘薇,林文翰,季宇彬.青龙衣毒性作用及体外抗肿瘤作用的试验研究[J].中国中药杂志,2004，29(9)：887-889. [6]CHEN R Z，MENG F L，LIU Z Q，et al.Antitumor activities of different fractions of polysaccharide purified from Ornithogal caudatum Ait [J].Carbohydrate Polymers，2010，80(3)：845-851. [7]LI J W，LIU Y F，FAN L P，et al.Antioxidant activities of polysaccharides from the fruiting bodies of Zizyphu jujuba cv.Jinsixiaozao [J].Carbohydrate Polymers，2011，84(1)：390-394. [8]KRATCHANOVA M，PAVLOVA E，PANCHEV I.The effect of microwave heating of fresh orange peels tissue and quality of extracted pectin [J].Carbohydrate Polymers，2004，56(2)：181-185. [9]孟繁磊,陈瑞战,张敏,等.刺五加多糖的提取工艺及抗氧化活性研究[J].食品科学,2010,31(10):168-174. [10]LI J W，DING S D，DING X L.Optimization of the ultrasonically assisted extraction of polysaccharides from Zizyphus jujuba cv.Jinsixiaozao [J].Journal of Food Engineering，2007，80(1)：176-183. [11]ZHAO S N，KWOK K C，LIANG H H.Investigation on ultrasound assisted extraction of saikosaponins from Radix Bupleuri[J].Separation and Purification Technology，2007，55(3)：307-312. [12]FILGUEIRAS A V，CAPELO J L，LAVILLA I，et al.Comparison of ultrasound assisted extraction and microwave-assisted digestion for determination of magnesium，manganese and zinc in plant samples by flame atomic absorption spectrometry [J].Talanta，2000，53(20)：433-441. [13]WANG J，ZHANG J，WANG X，et al.A comparison study on microwave-assisted extraction of Potentilla anserine L.polysaccharides with conventional method：Molecule weight and antioxidant activities evaluation[J].Carbohydrate Polymers，2010，80(1)：84-93. [14]VODENIC〖AKcˇD〗AROVA M，D〖AKrˇD〗IMALOV G，HROMADKOVA Z，et al.Xyloglucan degradation using different radiation sources：a comparative study[J].Ultrason Sonochem,2006，13(2)：157-164.
相似文献/References:
[1]李海平,何玥,闫军,等.响应面法优化超声提取地皮菜多糖工艺研究[J].北方园艺,2013,37(04):138.
[2]谭启明,付瑞敏,李景原,等.响应面法优化微波辅助提取芦荟凝胶多糖工艺[J].北方园艺,2014,38(13):133.
TAN Qi-ming,FU Rui-min,LI Jing-yuan,et al.Optimization of Microwave-assisted Extraction Process of Aloe barbadensis Miller Polysaccharies by Response Surface Method[J].Northern Horticulture,2014,38(22):133.
[3]徐 雷,刘常丽,张 群,等.不同初加工方法对茯苓多糖和三萜类成分的影响[J].北方园艺,2014,38(10):148.
XU Lei,LIU Chang-li,ZHANG Qun,et al.Effect of Different Processing Methods on Polysaccharides and Triterpenes of?Poria [J].Northern Horticulture,2014,38(22):148.
[4]罗 琴,林惠昆,管春平,等.正交实验法优化提取二型鳞毛蕨多糖工艺的研究[J].北方园艺,2013,37(22):146.
LUO Qin,LIN Hui-kun,GUAN Chun-ping,et al.Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment[J].Northern Horticulture,2013,37(22):146.
[5]陈玉琴,南海娟,刘坤峰.蕨菜多糖提取及抗氧化特性[J].北方园艺,2014,38(14):128.
CHEN Yu-qin,NAN Hai-juan,LIU Kun-feng.Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum [J].Northern Horticulture,2014,38(22):128.
[6]魏楠楠,姚 睿,李 阳,等.几种因素对反应器铁皮石斛原球茎生长及有效物质的影响[J].北方园艺,2014,38(14):156.
WEI Nan-nan,YAO Rui,LI Yang,et al.Effect of Several Factors on Accumulation of Biomass and Bioactive Compounds during Bioreactor Culture of Dendrobium candidum PLB[J].Northern Horticulture,2014,38(22):156.
[7]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(22):148.
[8]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(22):154.
[9]姜少娟.毛竹叶多糖超声提取工艺研究[J].北方园艺,2013,37(13):166.
JIANG Shao-juan.Research on Ultrasonic Wave Extraction of Polysaccharides from Phyllostachys pubescens Leaves[J].Northern Horticulture,2013,37(22):166.
[10]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135.
HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(22):135.
备注/Memo
第一作者简介：陆娟（1982-），女，博士，副教授，研究方向为天然有机化学。E-mail：lujuan0626@aliyun.com.基金项目：吉林省教育厅“十二五”科学技术资助项目（吉教科合字[2013]第255号）。