LU Na,ZHOU Zufa,WANG Weike,et al.Study on Identification of Produce Pleurolus eryngii Ouel by ISSR Molecular Marker[J].Northern Horticulture,2015,39(21):150-152.[doi:10.11937/bfyy.201521038]
利用ISSR分子标记鉴别杏鲍菇生产菌株的研究
- Title:
- Study on Identification of Produce Pleurolus eryngii Ouel by ISSR Molecular Marker
- Keywords:
- Pleurotus eryngii Quel; ISSR; mycelial growth rate; yield
- 文献标志码:
- A
- 摘要:
- 以15个杏鲍菇生产菌株为试材，采用ISSR分子标记鉴别方法，从52个ISSR通用引物中筛选出32个ISSR引物对其DNA进行PCR扩增，分析其DNA序列多态性；同时，比较研究了各菌株的菌丝长势及产量情况。结果表明：菌丝生长速度和产量没有表现出各类群的特点，杏鲍菇的菌丝生长速度和产量比较易受环境条件和基因显隐性的影响；参试菌株遗传相似系数变异范围为0.64~0.98，采用UPGMA分析表明，在0.87处15个杏鲍菇菌株可分为5类。
- Abstract:
- With 15 Pleurotus eryngii Quel varieties as test materials，using ISSR molecular marker identification method，32 ISSR primers were screened from 52 ISSR primers and the DNA was amplified by PCR，the DNA sequence polymorphism was analyzed；at the same time，the varieties of mycelial growth and yield were compared.The results showed that mycelial growth rate and yield did not exhibit the characteristics of each group，the effect of mycelial growth rate and yield were more susceptible to environmental conditions and dominant or recessive gene；reference strains of genetic similarity coefficient of variation ranged 0.64-0.98.Using the UPGMA analysis showed that，in 0.87 at 15 Pleurotus eryngii Quel varieties were divided into five types.
参考文献/References:
[1]陈土瑜.食用菌生产大全[M].北京:中国农业出版社,1988. [2]冯伟林,蔡为明,金群力,等.秀珍菇菌株主要农艺性状比较及ISSR分子标记鉴定[J].食用菌学报,2014,21(2):14-18. [3]黄志龙,肖淑霞,上官舟建.杏鲍菇优良菌株筛选及配套标准化栽培技术[J].食用菌,2008(2):25-27.
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备注/Memo
第一作者简介：陆娜（1983-），女，本科，农艺师，研究方向为食用菌栽培。E-mail：lulu_838700@163.com.责任作者：周祖法（1969-），男，本科，高级农艺师，研究方向为食用菌育种与栽培。E-mail：fage@hz.cn.基金项目：国家食用菌产业技术体系资助项目（CARS-24）；杭州市科技发展专项资金资助项目（20140432B11）。