[1]刘西周.一株野生灰树花菌丝体液体培养基正交方法筛选[J].北方园艺,2015,39(21):152-154.[doi:10.11937/bfyy.201521039]
　LIU Xizhou.Selection of Liquid Medium for a Wild Grifola frondosa Mycelium by Orthogonal Design[J].Northern Horticulture,2015,39(21):152-154.[doi:10.11937/bfyy.201521039]
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一株野生灰树花菌丝体液体培养基正交方法筛选

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第39卷 期数: 2015年21 页码: 152-154 栏目: 出版日期: 2015-11-15
Title:
Selection of Liquid Medium for a Wild Grifola frondosa Mycelium by Orthogonal Design
作者:
刘西周
（天津师范大学蕈菌研究所,天津市动植物抗性重点实验室,天津 300387）
Author(s):
LIU Xizhou
(Mushroom Institute，Tianjin Normal University，Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin 300387)
关键词:
灰树花; 液体培养基; 正交设计; 生物量
Keywords:
Grifola frondosa; liquid medium; orthogonal design; mycelium biomass
DOI:
10.11937/bfyy.201521039
文献标志码:
A
摘要:
以1株野生灰树花菌株为试材，采用正交实验设计法，研究了不同液体培养基对其菌丝体生物量的影响。结果表明：其最适配方为蔗糖25 g/L、玉米粉25 g/L（浸提液）、麦麸20 g/L（浸提液）、酵母粉2 g/L、MgSO4?7H2O 2.5 g/L、KH2PO4 5 g/L、维生素B1 0.030 g/L、pH自然，24℃培养静置培养10 d，其菌丝生物量为10.41 g/L。
Abstract:
Taking a wild Grifola frondosa strain as material，the effect of different liquid medium on mycelial biomass was studied by using orthogonal design method in this paper.The results showed that the optimum liquid medium composition were sucrose 25 g/L，wheat bran 20 g/L,MgSO4?7H2O 2.5 g/L，corn flour 4 g/L，yeast powder 2 g/L,KH2PO4 5 g/L and vitamin B1 0.030 g/L，natural pH，through culturing for 10 d at 24℃,the biomass of Grifola frondosa reached 10.41 g/L.

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备注/Memo

作者简介：刘西周（1984-），男，硕士，助理实验师，研究方向为蕈菌设施化培殖与植物组织培养。E-mail：fusant@126.com.

更新日期/Last Update: 2015-12-28