XIE Xiuhong,LI Wei,LI Cuilan,et al.Spore Propagation Techniques of Osmunda cinnamomea L.var.asiatica[J].Northern Horticulture,2015,39(20):27-30.[doi:10.11937/bfyy.201520006]
分株紫萁孢子繁殖技术
- Title:
- Spore Propagation Techniques of Osmunda cinnamomea L.var.asiatica
- 文献标志码:
- A
- 摘要:
- 以分株紫萁为试验材料，采用设置不同条件（营养基质、温度和光照）或浓度（KH2PO4、激素、水分、ABA）的方法，初步探讨了分株紫萁孢子萌发、配子体发育和诱导孢子体形成的培养条件，为其孢子人工快速繁殖提供理论依据。结果表明：以草炭土与保护地土1∶1混合为育苗基质，100℃高温灭菌后播孢，保持自然变温和昼夜交替光照条件；播孢3 d后，每隔3 d用喷雾法喷施1次50 mg/L 2,4-D，连续喷3次；每隔3 d用喷雾法喷施1次0.3% KH2PO4，连续喷5次，有利于孢子萌发和配子体发育。在配子体肉眼可见时，选取生长良好的配子体进行移栽，将基质含水量调到田间持水量，移栽1周后，每隔3 d用喷雾法喷施1次1.0 mg/L激素ABA，连续3次，有利于配子体形成孢子体和生长发育。
- Abstract:
- Culture conditions of spore germination，gametophyte growth and sporophyte transformation of Osmunda cinnamomea L.var.asiatica were preliminary discussed in this paper with different conditions(substrates,temperature,light) and different concentration of KH2PO4,hormone,water,ABA，to provide theoretical foundation of artificial rapid propagation techniques for making use of spores.The results showed that favorable conditions of spore germination and gametophyte development were as follows：a part of protective ground soil and a part of peat soil were mixed as seeding substrate，spores were sowed after 100℃ high temperature sterilization with maintaining conditions of natural temperature and alternating day and night lighting.3 days after sowing spore，spraying 2,4-D once every 3 days，continuous sprayed 3 times.Spraying 0.3% KH2PO4 once every 3 days，continuous sprayed 5 times.Favorable conditions of sporophyte transformation and development were as follows：when gametophyte was saw by naked eye，transplanting good gametophyte，adjusting water content of substrate to field capacity.A week after transplanting，spraying 1.0 mg/L hormone ABA once every 3 days，continuous sprayed 3 times.
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备注/Memo
第一作者简介：谢修鸿（1972-），女，吉林长春人，博士，讲师，现主要从事农林废弃物利用与土壤改良等研究工作。E-mail：yuxieaoran@163.com.责任作者：梁运江（1972-），男，吉林前郭人，博士，副教授，现主要从事土壤与植物营养等研究工作。E-mail:lyjluo@ybu.edu.cn.基金项目：国家科技支撑计划资助项目（2013BAD07B02）；吉林省延边朝鲜族自治州科学技术局资助项目(2008YB501A12)。