WANG Linshan,SONG Cengxiao,SONG Jianjun,et al.Screening of SCAR Molecular Markers Linked to Ty-2 Gene Resistant to Tomato Yellow Leaf Curl Disease[J].Northern Horticulture,2015,39(15):94-99.[doi:10.11937/bfyy.201515025]
番茄黄化曲叶病毒抗病基因Ty-2的SCAR标记筛选
- Title:
- Screening of SCAR Molecular Markers Linked to Ty-2 Gene Resistant to Tomato Yellow Leaf Curl Disease
- Keywords:
- tomato; tomato yellow leaf curl disease; Ty-2 gene; molecular marker
- 文献标志码:
- A
- 摘要:
- 以抗病杂合体(Ty-2/ty-2)、抗病纯合体(Ty-2/Ty-2)和感病纯合体(ty-2/ty-2)番茄为试材，以提取植物的基因组DNA作为模板，根据与抗病基因Ty-2紧密连锁的RFLP标记设计特异引物，采用DNA聚合酶链式反应的方法，筛选获得了2个与抗病基因Ty-2紧密连锁的、且稳定可靠的SCAR标记，分别命名为T0302-1和T0302-2。结果表明：T0302-1和T0302-2两个标记均为共显性分子标记，可用于番茄抗病育种的辅助选择中。该研究旨在筛选获得与番茄黄化曲叶病毒病抗病基因Ty-2紧密连锁的分子标记，为番茄抗病育种提供技术支撑。
- Abstract:
- Resistant heterozygotes (Ty-2/ty-2)，resistant homozygotes (Ty-2/Ty-2) and susceptible homozygotes (ty-2/ty-2) were used as experimental materials.Based on the published RFLP markers tightly linked to Ty-2，three pairs of specific primers were designed for PCR amplification.As a result，two steady-going and effective SCAR markers，T0302-1 and T0302-2，were found.The results showed that T0302-1 and T0302-2 were co-dominant SCAR markers，which could be used in marker assistant selection for tomato disease resistance breeding.This paper was aimed to obtain molecular markers tightly linked to Ty-2 gene resistant to tomato yellow leaf curl virus and afford technical supports for tomato disease resistance breeding.
参考文献/References:
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备注/Memo
第一作者简介：王琳珊（1986-），女，河北石家庄人，硕士研究生，研究方向为番茄抗病基因分子标记。E-mail：wanglinshanwls@163.com. 责任作者：宋建军（1963-），男，河北宁晋人，博士，教授，硕士生导师，现主要从事番茄抗病育种等研究工作。E-mail：songjj63@126.com. 基金项目：河北省科技支撑计划资助项目（14226310D）。