XU Bin,PENG Lixia,PAN Wen,et al.Comparative Study on Methods for Testing Pollen Viability of Camellia azalea[J].Northern Horticulture,2015,39(13):88-90.[doi:10.11937/bfyy.201513026]
杜鹃红山茶花粉活力测定方法的比较研究
- Title:
- Comparative Study on Methods for Testing Pollen Viability of Camellia azalea
- 文章编号:
- 1001-0009(2015)13-0088-03
- 分类号:
- S 685.14
- 文献标志码:
- A
- 摘要:
- 以4年生嫁接的杜鹃红山茶花粉为试材,采用MTT法、TTC法和离体萌发法,研究了不同测定方法对杜鹃红山茶花粉活力的影响。结果表明:不同测定方法对杜鹃红山茶花粉活力测定结果影响较大,其中,离体萌发法是杜鹃红山茶花粉活力的最佳检测方法,30℃条件下花粉萌花最适培养基为2.5%琼脂+100 g/L蔗糖+10 mg/L硼酸,适宜统计的培养时间为4 h,花粉萌发率达58.10%,Ca2+对花粉萌发起抑制作用;MTT染色得出92.5%的杜鹃红山茶花粉具有活力,明显高于离体萌发法,而TTC法由于染色不清晰,不适合用于杜鹃红山茶花粉活力检测。
- Abstract:
- Taking 4-year-old grafted Camellia azalea as test material,effects of different methods on the viability of pollens collected from Camellia azalea were investigated,using the MTT staining method,TTC staining method and pollen germination in vitro method. The results showed that the viability of pollens was associated with the testing method,the pollen germination in vitro was the optimum method to determine pollen viability of C.azalea. The pollen germinating rate reached the highest on the media supplemented with 2.5% agar,100 g/L sucrose and 10 mg/L boric acid under 30℃ after 4 hours.However,adding the Ca2+ on the media would prevent the pollen germinating.92.5% pollen had viability in MTT solution,significantly higher than by pollen germination in vitro.TTC staining method was unsuited for detection the pollen viability of C.azalea,for its dying unclearly and lower viability rate.
参考文献/References:
[1]高继银,Clifford R P,杜跃强.山茶属植物主要原种彩色图集[M].杭州:浙江科学技术出版社,2005:34-35.[2]]李天菲,林田,徐碧玉,等.杜鹃红山茶花粉萌发力及贮藏耐性的研究[J].生物技术通报,2008(增刊):239-243.[3]严丹峰,李建光,许宇山,等.杜鹃红山茶花粉活力与柱头可授性研究[J].北方园艺,2013(2):71-73.[4]刘玉玲,潘文,张方秋,等.杜鹃红山茶花粉保存及其生活力测定[J].广东林业科技,2010,26(2):1-6.[5]赵鸿杰,乔龙巴图,殷爱华,等.3种山属属植物花粉活力测定方法的比较[J].中南林业科技大学学报,2010,30(3):105-107.[6]左丹丹,明军,刘春,等.植物花粉生活力检测技术进展[J].安徽农业科学,2007,35(16):4742-4745.[7]张超仪,耿兴敏.六种杜鹃花属植物花粉活力测定方法的比较研究[J].植物科学学报,2012,30(1):92-99.[8]罗晓莹.杜鹃红山茶保护生物学研究[D].广州:华南农业大学,2006.[9]汤红明,徐冬青,徐根娣,等.植物花粉萌发的研究进展[J].安徽农业科学,2006,34(24):6436-6438.[10]朱展望,张改生,牛娜.小麦花粉的离体萌发研究[J].麦类作物学报,2007,27(1):12-15.[11]白天,解玮佳,李世峰,等.糖、硼、钙对大喇叭杜鹃四合花粉萌发的影响[J].上海交通大学学报(农业科学版),2013,31(6):48-54.[12]刘赢男,周丹,刘伟,等.不同培养基及贮藏条件对山梅花属植物花粉生活力的影响[J].东北林业大学学报,2011,39(12):47-49.[13]车代弟,樊金萍,王金刚.东方百合花粉萌发培养基组分的优化[J].植物研究,2003,23(2):178-181.[14]Tian H Q,Zhang Z,Russell S D.Isolation of the male germ unit organization and function in tobacco(Nicotiana tabacum L.)[J].Plant Cell Reports,1998,18(12):143-147.[15]贾文庆,刘宇,陈韵,等.Ca2+与葱兰花粉萌发和花粉管生长的关系[J].西北林学院学报,2007,22(4):98-99.[16]Malho R,Read N D,Trewavas A J,et al.Calcium channel activity during pollen tube growth and reorientation[J].The Plant Cell,1995,7(8):1173-1184.[17]罗凤霞,周威,杨春起,等.水仙花粉离体萌发温度和培养液研究[J].辽宁林业科技,2007(5):13-16.[18]Graaf B H,Derksen J W,Marianic.Pollen and pistil in the programic phase[J].Sex Plant Report,2001,14:41-55.[19]周莉花,郝日明,赵宏波.蜡梅花粉活力检测方法筛选及保存时间观察[J].浙江林学院学报,2006,23(3):270-274.[20]杨秀莲,向其柏.桂花花粉活力测定与‘晚籽银’桂柱头可授性分析[J].林业科技开发,2007,21(3):22-25.
相似文献/References:
[1]朴 锦,王 坤,范惠明,等.关苍术的开花动态和花蕊形态及花粉活力研究[J].北方园艺,2014,38(12):142.
PIAO Jin,WANG Kun,FAN Hui-ming,et al.Reseach on Flowering Dynamic,Flower Morphology and Pollen Viability of Atractylodes japonica [J].Northern Horticulture,2014,38(13):142.
[2]严婷,郭军战,徐龙光.文冠果优良单株开花生物学特性研究[J].北方园艺,2014,38(05):58.
YAN Ting,GUO Jun-zhan,XU Long-guang.Research on Flowering Biological Characteristics of Superior Individual Plant of Xanthoceras sorbifoliaBunge[J].Northern Horticulture,2014,38(13):58.
[3]于安民,杨锦芬,詹若挺,等.贮存条件对阳春砂花粉活力的影响[J].北方园艺,2013,37(10):147.
YU An-min,YANG Jin-fen,ZHAN Ruo-ting,et al.Effect of Different Preservation Conditions on Pollen Viability of Amomum villosum Lour.[J].Northern Horticulture,2013,37(13):147.
[4]马建琴,郭太君,陈少鹏,等.野生细叶百合开花生物学研究[J].北方园艺,2013,37(23):88.
MA Jian-qin,GUO Tai-jun,CHEN Shao-peng,et al.Study on Blossom Biology of Wild Lilium pumilum[J].Northern Horticulture,2013,37(13):88.
[5]高兴颖,崔世茂,付崇毅,等.温室栽培砂糖橘花粉活力测定方法比较研究[J].北方园艺,2014,38(03):39.
GAO Xing-ying,CUI Shi-mao,FU Chong-yi,et al.Comparison Study on Pollen Viability Determining Methods of Citrus reticulate ‘Shatangju’ in Solar Greenhouse[J].Northern Horticulture,2014,38(13):39.
[6]施海燕,呼丽萍.大樱桃不同品种花器官抗寒性的初步研究[J].北方园艺,2012,36(02):5.
SHI Hai-yan,HU Li-ping.A Preliminary Study on The Cold Resistance of Different Prunus avium L. Cultivar[J].Northern Horticulture,2012,36(13):5.
[7]杨芩,柏兰平,张杰,等.花龄对火棘柱头可授性及花粉活力的影响[J].北方园艺,2014,38(20):18.
YANG Qin,BO Lan-ping,ZHANG Jie,et al.Effect of Fowler Age on Stigma Receptivity and Pollen Viability in Pyracantha Roem.[J].Northern Horticulture,2014,38(13):18.
[8]杨 芩,唐 露,李性苑,等.阴雨对蓝莓花粉活力和柱头可授性的影响[J].北方园艺,2015,39(03):47.[doi:10.11937/bfyy.201503014]
YANG Qin,TANG Lu,LI Xing-yuan,et al.Effect of Rainy on Stigma Receptivity and Pollen Viability in Blueberry[J].Northern Horticulture,2015,39(13):47.[doi:10.11937/bfyy.201503014]
[9]杨红,余和明,李小艳,等.猕猴桃花粉生活力测定方法及花药处理方法研究[J].北方园艺,2015,39(08):36.[doi:10.11937/bfyy.201508009]
YANG Hong,YU He-ming,LI Xiao-yan,et al.Study on the Method of Measuring Pollen Viability and Pretreatment of Pollen[J].Northern Horticulture,2015,39(13):36.[doi:10.11937/bfyy.201508009]
[10]宋怀磊,程慧,李亮,等.蜜蜂采集梨花粉萌发活性及应用分析[J].北方园艺,2016,40(12):31.[doi:10.11937/bfyy.201612008]
SONG Huailei,CHENG Hui,LI Liang,et al.Study on the Germinability and Application of Bee-collected Pear Pollens[J].Northern Horticulture,2016,40(13):31.[doi:10.11937/bfyy.201612008]
[11]严丹峰,李建光,许宇山,等.杜鹃红山茶花粉活力与柱头可授性研究[J].北方园艺,2013,37(02):68.
YAN Dan-feng,LI Jian-guang,XU Yu-shan,et al.Study on Pollen Viability and Stigma Receptivity of Camellia azalea[J].Northern Horticulture,2013,37(13):68.
备注/Memo
第一作者简介:徐斌(1974-),男,博士,教授级高级工程师,现主要从事观赏植物研究与开发利用等工作。E-mail:xubin@sinogaf.cn.基金项目:国家“十二五”农村领域国家科技计划子研究资助项目(2012BAD01B0703-2)。