CHEN Jin-e,WANG Qing-hong,WEI Zeng-yun,et al.Optimization of Ultrasound-assisted Extraction of Manyprickle acathopanax Root Polysaccharides Using Response Surface Methodology[J].Northern Horticulture,2015,39(07):123-127.[doi:10.11937/bfyy.201507036]
响应面法优化刺五加中多糖的超声提取工艺
- Title:
- Optimization of Ultrasound-assisted Extraction of Manyprickle acathopanax Root Polysaccharides Using Response Surface Methodology
- 文献标志码:
- A
- 摘要:
- 以刺五加为试材，利用超声辅助提取技术对刺五加多糖的提取工艺进行研究。在单因素试验的基础上，选择超声温度、时间、液固比、功率4个因素，通过Box-Behnken中心组合设计试验和响应面分析法，模拟得到二次多项式回归方程的预测模型。结果表明：超声功率90 W，提取温度为57℃，液固比40∶1 mL/g，提取时间42 min是刺五加多糖的最佳超声提取工艺。验证性试验表明，刺五加多糖得率为3.86%，与理论预测值3.88%相符。用超声法对比研究了传统水煮法提取刺五加多糖的优劣，超声法不仅能耗低、产率高，且提取时间缩短11倍。
- Abstract:
- Taking Manyprickle acathopanax as test material，the polysaccharides was extracted by ultrasound-assisted extraction technology.The quadratic polynomial regression equation prediction model was obtained by using the Box-behnken central composite test and response surface analysis based on the single factor experiments，such as ultrasound power (A)，temperature (B)，liquid-solid ratio (C)，and the ultrasound (D).The results showed that the optimal conditions of ultrasound-assisted extraction of Manyprickle acathopanax polysaccharides were as follows,ultrasound power of 90 W，extraction temperature of 57℃，40∶1 mL/g of the ratio of liquid to solid and time of 42 minutes.Under these conditions，the experimental yield of Manyprickle acathopanax polysaccharides was 3.86%，which was well matched with the value predicted (3.88%) by the model.Ultrasonic extraction method was not only low energy consumption，high yield，and the extraction time was shortened by 11 times compared with the traditional water-boiling method.
参考文献/References:
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备注/Memo
第一作者简介：陈金娥（1957-），女，本科，高级实验师，研究方向为天然植物分离及分析。责任作者：张海容（1957-），男，博士，教授，研究方向为天然植物分离及分析。E-mail：hairong1015@163.com. 基金项目：山西省教育厅重点建设学科资助项目（20141010）；山西省教育厅国际交流资助项目（2011-63）；山西省高等学校教学改革资助项目（2014-38）；忻州师范学院应用化学创新实践基地资助项目（2013-31）。