LIU Li-jie,LIN Li-dong,ZHANG Dong-xiang.Analysis on Esterase Isozemes and Peroxidase Isozymes in Singlecell Clone of Scutellaria Callus[J].Northern Horticulture,2015,39(07):85-90.[doi:10.11937/bfyy.201507026]
黄芩单细胞克隆愈伤组织酯酶同工酶及过氧化物同工酶酶谱分析
- Title:
- Analysis on Esterase Isozemes and Peroxidase Isozymes in Singlecell Clone of Scutellaria Callus
- 文献标志码:
- A
- 摘要:
- 以黄芩单细胞克隆愈伤组织为试材，采用聚丙烯酰胺凝胶电泳（PAGE）法，通过分析酯酶同工酶（EST）和过氧化物同工酶(POD)的酶带,研究黄芩单细胞克隆愈伤组织与亲本愈伤组织的遗传特性与品系差异。结果表明：黄芩不同品系单细胞克隆愈伤组织EST谱带间具3个带区特征，最多谱带数品系为4条，最少谱带数品系为3条，其中1号、2号、3号谱带为黄芩单细胞克隆愈伤组织的特征谱带；POD谱带间具4个带区特征，其中1号、3号和7号酶带为特征谱带，EST和POD谱带的有无、宽窄、颜色深浅，相对迁移率大小均不尽相同。黄芩单细胞克隆愈伤组织与亲本细胞愈伤组织既具有亲缘特征又具有品系差异，以期为单细胞克隆品系的遗传特性和品系鉴别提供理论参考。
- Abstract:
- Taking single-cell clone Scutellaria baicalensis callus as material，using the polyacrylamide gel electrophoresis (PAGE) method，through the analysis of esterase isozyme (EST) and peroxidase isozyme (POD) enzyme band，Radix scutellariae singlecell clone callus and the genetic characteristics of callus and strain differences in parents were studied.The results showed that three bands were characteristics in EST and four bands were characteristics in POD in single-cell clone Scutellaria baicalensis callus of different strains，four bands were the most and three bands were the lest in EST in different strains，the first，the second and the third brands were the characteristic band；while the first，the third and the seventh brands were the characteristics bands in POD；differences were found in the presence or absence the number，width，color depth，the relative mobility of the same bands of EST and POD.There exist both genetic characteristics and strains difference in the single-cell clones Scutellaria callus and the parental cells，and these characteristics provide a theoretical reference in genetic characteristics and strains identification of the single-cell clone Scutellaria baicalensis (HQSC) callus.
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备注/Memo
第一作者简介：刘丽杰（1980-），女，博士，讲师，研究方向为药用植物抗性生理与次生代谢产物及分子生物学。E-mail:liulijie2001@163.com. 责任作者：张东向（1963-），男，硕士，教授，研究方向为植物生理及植物细胞工程。E-mail:zhangdx1019@163.com. 基金项目：黑龙江省自然科学基金资助项目(C201013)；黑龙江省教育厅资助项目(12521609)。