ZHANG Wen-long.PAGE Analysis of Genetic Diversity of Dendrobium Germplasm Resources[J].Northern Horticulture,2014,38(14):104-106.
石斛种质资源遗传多样性的PAGE分析
- Title:
- PAGE Analysis of Genetic Diversity of Dendrobium Germplasm Resources
- 文章编号:
- 1001-0009(2014)14-0104-03
- Keywords:
- Dendrobium; ISSR; PAGE
- 分类号:
- R 931.71
- 文献标志码:
- A
- 摘要:
- 以16种石斛属植物为试材，采用PAGE法，利用筛选获得的6条理想ISSR多态性引物，研究了小分子量片段的差异对石斛属植物分类的影响。结果表明：试验结果与经典形态分类相似，但也有一定差别；PAGE法多态性比率高，可进一步从分子水平揭示石斛属植物的遗传多样性与亲缘关系。
- Abstract:
- Taking 16 Dendrobium germplasm resources as experimental materials,using polyacrylamide gel eletrophoresis(PAGE) methods,effect of difference of low molecular weight bands on plant taxonomy for?Dendrobium??were studied by 6 ISSR primers screened.The results showed that the results of the experiment was basically similar to the classical morphological classification.But there were some difference between both methods.PAGE method had high polymorphism ratio and could be used to further reveal genetic diversity and relationship of?Dendrobium??at the molecular level.
参考文献/References:
[l]徐琼，陈素红，吕圭源．3种不同石斛的化学成分及相关药理学研究进展[J].亚太传统医药，2010，6(4)：115-118.
[2]胡之壁．中药现代生物技术[M].北京：人民出版社，2009：97-100.
[3] Saghai-Maroof M A. Soliman K M, Jorgensen R A, et aL RibosomalDNA spacerlength polymorphisms in barley: Mendelian inheritance. chromo-somal location, and population dynamics [J]. Proceedings of the National Academy of Sciences,1984, 81(24): 8014-8018.
[4]赵树进，中药材生物多样性及核酸分析技术[M]．北京：科学出版社，2009：64-70．
[5]吉占和，陈心启，罗毅波，等，中国植物志[M]．19卷．北京：科学出版社，1999：67-257．
[6]任羽，杨光穗，尹俊梅，等，石斛种质资源遗传多样性的RAPD分析[J]．热带农业科学，2007，23(6)：598-600．
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备注/Memo
作者简介：张文龙(1976-)，男，博士，副教授，现主要从事药用植物栽培育种与分子生物学等研究工作。E-mail:wlzhsst@yeah.net.