HU Xuan-ping.Study on Effect of Different Growth-regulators on Callus Induction of Cotyledon Explants About Critus rutaceae [J].Northern Horticulture,2014,38(09):109-112.
不同生长调节因子对耐寒柑橘愈伤组织诱导效应研究
- Title:
- Study on Effect of Different Growth-regulators on Callus Induction of Cotyledon Explants About Critus rutaceae
- 文章编号:
- 1001-0009（2014）09-0109-04
- Keywords:
- Citrus rutaceae; cotyledon; growth-regulators; callus
- 分类号:
- S 666.2
- 文献标志码:
- A
- 摘要:
- 以耐寒柑橘子叶为试验材料，研究了不同浓度的2,4-D、NAA、6-BA与KT对柑橘子叶愈伤组织诱导的影响作用。结果表明：2,4-D、NAA、6-BA与KT对柑橘子叶愈伤组织诱导具有重要效应，其综合贡献率分别为86.4%、93.9%、93.9%与94.5%。4种生长调节因子对柑橘子叶愈伤组织诱导的膨大率、膨大速度、出愈率、出愈速度的效应大小分别为2,4-D>NAA>6-BA>KT、KT>2,4-D>NAA>6-BA、2,4-D>NAA>6-BA>KT与2,4-D>KT>NAA>6-BA。综合考虑测量指标效应，对于柑橘子叶愈伤组织诱导的最适生长调节因子组合配比为2,4-D 1.0 mg/L+NAA 2.0 mg/L+6-BA 0.5 mg/L+KT 0.5 mg/L。
- Abstract:
- Taking Citrus rutaceae leaves as materials,the effect of 2,4-D,NAA,6-BA,KT on the callus induction was researched.The results showed that,NAA,2,4-D,6-BA and KT had significant influence on callus induction from cotyledon explants and they contributed to callus induction for each dependent variable were 86.4%, 93.9%, 93.9% and 94.5%.The sequences that four growth-regulators exerted effect on expanding rate,expanding velocity，callus inducing rate and callus accumulating velocity were 2,4-D>NAA>6-BA>KT，KT>2,4-D>NAA>6-BA，2,4-D>NAA>6-BA>KT,2,4-D>KT>NAA>6-BA.Taking the comprehensive effect of various measurement variables into consideration,the appropriate culture condition about four growth-regulators combining for cotyledon explants of Citrus rutaceae was 2,4-D 1.0 mg/L+NAA 2.0 mg/L+6-BA 0.5 mg/L+KT 0.5 mg/L.
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备注/Memo
作者简介：胡选萍（1975-），女,硕士，讲师，现主要从事植物生物技术研究工作。E-mail:huxuanping@163.com.