HU Xuan-ping.Effect of Two Different Growth-regulators Combination on Callus Induction of Amorphophallus[J].Northern Horticulture,2014,38(07):96-99.
两种不同激素组配方式对魔芋愈伤的诱导效应
- Title:
- Effect of Two Different Growth-regulators Combination on Callus Induction of Amorphophallus
- 文章编号:
- 1001-0009(2014)07-0096-04
- Keywords:
- Amorphophallus; growth-regulators combination; callus
- 分类号:
- Q 939
- 文献标志码:
- A
- 摘要:
- 以魔芋芽鞘为试材,研究了2种不同激素组配方式对魔芋芽鞘外植体脱分化与愈伤组织诱导的效应。结果表明:2种不同激素组配方式对魔芋芽鞘外植体脱分化启动与愈伤组织诱导效果差异明显,表现在无论静态的膨大率与愈伤诱导率,还是动态的出愈速度差异均处于显著水平(P<0.05或P<0.01);综合各种效应变量,培养基中6-BA∶NAA为1.0∶2.0或KT∶2,4-D为0.5∶2.0 或1.0∶2.0是魔芋芽鞘外植体诱导愈伤组织的合适激素配比条件。
- Abstract:
- Taking Amorphophallus as material,the effect of the two growth-regulators combination exerted on callus induction from coleoptile explants of Amorphophallus were researched.The result showed that the two growth-regulators combination had significant influence on callus induction from coleoptile explants both in static variable of the rate of expanding and callus induction and dynamic ratio of the speed of callus induction (P<0.05 or P<0.01).Taking the comprehensive effect into consideration,the appropriate growth-regulators combination for callus inducing were 6-BA∶NAA=1.0∶2.0,KT∶2,4-D=0.5∶2.0 or KT∶2,4-D=1.0∶2.0.
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备注/Memo
作者简介:胡选萍(1975-),女,硕士,讲师,现主要从事植物细胞工程等研究工作。E-mail:huxuanping@163.com.