ZHANG Xue,LI Shuang,HAN Yu-zhu.Study on Trueness and Purity Identification of Seeds in Common Bean[J].Northern Horticulture,2014,38(07):91-95.
利用种子蛋白SDS-PAGE电泳谱带鉴定菜豆品种真实性与纯度的研究
- Title:
- Study on Trueness and Purity Identification of Seeds in Common Bean
- 文章编号:
- 1001-0009（2014）07-0091-05
- Keywords:
- Phaseolus vulgaris L.; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; trueness; purity
- 分类号:
- S 643.1
- 文献标志码:
- A
- 摘要:
- 以不同采收年限的17个菜豆品种的种子为试材，采用SDS-PAGE电泳方法获得菜豆种子贮藏蛋白的电泳图谱，比较分析其真实性；同时研究种子蛋白SDS-PAGE技术鉴定菜豆品种真实性和纯度的可行性。结果表明：17个菜豆品种可以通过SDS-PAGE方法得到特征谱带A、B、C、D、E、F、G、H，且各品种谱带差异明显；SDS-PAGE蛋白质电泳方法用于鉴定菜豆品种真实性和种子纯度是可行的。
- Abstract:
- Taking seeds of seventeen common bean in diffrent recovery years as materials,the seeds storage proteins with SDS-PAGE electrophoresis were analysized.The feasibility of trueness and purity identification of seeds in common bean by SDS-PAGE ptotein electrophoresis bands were studied.The results shouwed that the unique bands were A，B，C，D，E，F,G and H for seventeen common bean,and there were significant difference among species.It was possible that SDS-PAGE could be used to identified the purity and trueness of seventeen landraces of common beans.
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备注/Memo
第一作者简介：张雪（1987-），女，吉林省吉林市人，硕士研究生，研究方向为蔬菜遗传育种与生物技术。E-mail：494539447@qq.com.