[1]高兴颖,崔世茂,付崇毅,等.温室栽培砂糖橘花粉活力测定方法比较研究[J].北方园艺,2014,38(03):39-41.
　GAO Xing-ying,CUI Shi-mao,FU Chong-yi,et al.Comparison Study on Pollen Viability Determining Methods of Citrus reticulate ‘Shatangju’ in Solar Greenhouse[J].Northern Horticulture,2014,38(03):39-41.
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温室栽培砂糖橘花粉活力测定方法比较研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年03期 页码: 39-41 栏目: 出版日期: 2014-02-10
Title:
Comparison Study on Pollen Viability Determining Methods of Citrus reticulate ‘Shatangju’ in Solar Greenhouse
文章编号:
1001-0009（2014）03-0039-03
作者:
高兴颖1; 崔世茂1; 付崇毅2; 刘 微1; 褚义红1; 朱山川1
1.内蒙古农业大学 农学院，内蒙古 呼和浩特 010019；
2.内蒙古农牧业科学院 蔬菜研究所，内蒙古 呼和浩特 010031
Author(s):
GAO Xing-ying1; CUI Shi-mao1; FU Chong-yi2; LIU Wei1; CHU Yi-hong1; ZHU Shan-chuan1
1.College of Agronomy，Inner Mongolia Agricultural University，Hohhot，Inner Mongolia 010019；
2.Vegetable Institute，Inner Mongolia Academy of Agriculture and Animal Husbandry，Hohhot，Inner Mongolia 010031
关键词:
砂糖橘; 花粉活力; 离体萌发法; MTT染色法; TTC染色法; I2-KI染色法
Keywords:
Citrus reticulate ‘Shatangju’; pollen viability; in vitro pollen germination method; MTT staining method; TTC staining method; I2-KI staining method
分类号:
S 666.2
文献标志码:
B
摘要:
以5 a生枳砧砂糖橘嫁接苗为试材，研究比较了离体萌发法、TTC染色法、I2-KI染色法和MTT染色法对花粉活力的测定效果及其对砂糖橘有叶花和无叶花2种类型离体花粉活性的影响。结果表明：砂糖橘无叶花花粉活性高于有叶花花粉活性；MTT染色法是简单快速测定砂糖橘花粉活性的最佳方法
Abstract:
Taking grafted seedings of Citrus reticulate ‘Shatangju’ on rootstock of trifoliate orange as material，the determination effects of I2-KI,TTC,MTT and in vitro pollen germination method were compared and their effect on in vitro activity of pollen between the different types of flower(the flower with leaves and non-leaf flower) of Citrus reticulate ‘Shatangju’ were studied.The results indicated that the pollen of Citrus reticulate ‘Shatangju’ viability of non-leaf flower was higher than the pollen viability of flower with leaves.MTT staining method was the optimum and rapid staining method to determine pollen viability of Citrus reticulate ‘Shatangju’.

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备注/Memo

第一作者简介：高兴颖（1987-），女，内蒙古通辽人，硕士研究生，研究方向为设施园艺及抗逆生理。E-mail：gaoxingyinga@163.com.

责任作者：崔世茂（1961-），男，山西代县人，教授，博士生导师，现主要从事园艺作物抗逆生理和园艺设施及环境调控研究等工作。E-mail：cuishimao@sina.com.
基金项目：内蒙古科技厅科技推广资助项目（20110710）

更新日期/Last Update: 2014-08-21