LI Na,LIU Jin-xia,DU Wen-jing,et al.Mutagenesis of Bacillus subtilis with High Toxicity and Strong Resilience，and Its Effect of Control of Botrytis cinerea of ‘Poinsettia’[J].Northern Horticulture,2014,38(23):101-105.
生防枯草芽孢杆菌高效抗逆菌株选育及其对一品红灰霉病的防控效果
- Title:
- Mutagenesis of Bacillus subtilis with High Toxicity and Strong Resilience，and Its Effect of Control of Botrytis cinerea of ‘Poinsettia’
- 文章编号:
- 1001-0009(2014)23-0101-05
- Keywords:
- Bacillus subtilis; high toxicity; resilience; breeding; Botrytis cinerea Pers.; ‘Poinsettia’
- 分类号:
- S 685.23；S 476
- 文献标志码:
- B
- 摘要:
- 采用UV和NTG复合诱变的方法对原始生防枯草芽孢杆菌Bs-a进行了诱变选育；同时结合抗逆能力和室内外抑菌活性测定，以期筛选出抑菌活性和抗逆性均较强的优良灰霉菌生防菌株。结果表明：通过UV和NTG复合诱变的方法获得了枯草芽孢杆菌Bs-a的突变菌株D17和W03，2种菌株具有传代稳定、高毒力、强抗逆的特点，其抗紫外线能力极强，经过2.0 h的紫外照射活菌浓度仍可达108 cfu/mL以上；可耐受的温度和pH值范围分别为0~60℃、pH 3~9，温室内对一品红灰霉病的相对防效可达85%以上，与出发菌Bs-a之间存在极显著差异，是2株具备产业化开发价值的灰霉病生防菌株。
- Abstract:
- Taking the mutagenesis of Bacillus subtilis Bs-a by UV and NTG combined with the test of resilience and indoor and outdoor antibacterial activity，filter out two superior strains of bacteria D17 and W03，which had stable inheritance high toxicity，strong resilience.The results showed that the anti-UV ability of the bacterias were strong，the living bacteria concentration of which remained viable up to 108 cfu/mL or more after ultra-vioket radiation for 2 hours.The range of withstand temperature and pH were 0-60℃ and pH 3-9，Their control effect of Botrytis cinerea of “Poinsettia” were more than 85%.It was significantly different with original strain Bs-a.D17 and W03 were industrial development valuable in microbial biocontrol bacteria.
参考文献/References:
[1]Xu J,Chen S W,Yu Z N.Optimization of process parameters for poly γ -glutamate production under solid state fermentation from Bacillus subtilis CCTCC202048[J].Process Biochemistry,2005(40):3075-3081. [2]彭研,陈相艳,裘纪莹,等.生防芽孢杆菌的研究进展[J].山东农业科学,2013,45(7):138-140. [3]杨佐忠,叶建仁.枯草芽孢杆菌PRS5菌剂控制水果贮藏期病害的研究[J].南京林业大学学报(自然科学版),2006,30(1):89-92. [4]穆常青,潘玮,蒋细良,等.枯草芽孢杆菌对稻瘟病的防治效果评价及机制初探[J].中国生物防治,2006,22(2):158-160. [5]Chen Z，Li Q，Liu H，et al.Greater enhancement of Bacillus subtilisspore yields in submerged cultures by optimization of medium composition through statistical experimental designs[J].Applied Microbiology and Biotechnology，2010，85:1353-1360. [6]黄现青,陆兆新,崔保安,等.枯草芽孢杆菌fmbJ产生的新型抗微生物物质体外抗NDV和IBDV的活性分析[J].生物工程学报,2006，22(2):328-333. [7]Moyne A L，Cleveland T E，Tuzun S.Molecular characterization and analysis of the operon encoding the antifungal lipopeptide bacillomycin D.FEMS[J].Microbiology Letters,2004,234:43-49. [8]孔建,赵白鸽,王文夕,等.枯草芽孢杆菌(Bacillus subtilis(Cohen)） B-903菌株抗菌物质对植物病原真菌的抑制作用[J].植物病理学报,1999,25(1):69-72. [9]孙广宇,宗兆锋.植物病理学实验技术[M].北京:中国农业出版社,2002:143-145. [10]Zhang C Q，Yuan S k，Sun H Y,et al.Sensitivity of Botrytis cinereafrom vegetable greenhouse to boscalid [J].Plant Pathology,2007,56:646-653. [11]阳征助.花卉灰霉病的发病症状与综合防治[J].现代农业科技,2007(7):50-51. [12]张建军.灰葡萄孢对腐霉利的抗性变异及抗性菌株自然适合度研究[D].扬州:扬州大学,2004. [13]李斌.一品红叶斑病病原学及其拮抗细菌的研究[D].杭州:浙江大学,2005. [14]王万磊.城市园林植物病虫害生态控制探析[J].防护林科技,2014,126(3):48-49. [15]夏文胜.武汉市园林植物病虫害可持续控制的对策[J].园林科技,2009(3):10-17. [16]沈普良.农药生物测定[M].北京：中国农业出版社,2013:87-88. [17]农业部农药检定所生测室.农药田间药效试验准则[S].北京:中国标准出版社,2000:190-193. [18]梁亮,邱雁临,许进涛.紫外线与亚硝酸钠复合诱变选育L组氨酸产生菌[J].微生物学杂志,2008,28(2):27-29. [19]陈永浩,王强.透明质酸生产菌的诱变选育[J].微生物学通报,2009,36(2):205-210. [20]邱思鑫,黄志鹏,黄必旺,等.添加剂对苏云金杆菌杀虫效果的影响[J].武夷科学,2004,20(12):62-66. [21]周燚,王中康,喻子牛.微生物农药研发与应用[M].北京:化学工业出版社,2006：159-166. [22]曹坳程.农药大田药效试验及市场开发[M]∥陈万义,王根龙,李钟华.新农药的研发-方法·进展.北京:化学工业出版社,2007:166-186.
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备注/Memo
第一作者简介：李娜（1984-），女，本科，助理研究员，研究方向为生防微生物资源研究及其产品开发。E-mail:sherry0605@sohu.com.