SONG Jian-kun,HUANG Man-na,WANG Ran.Research on in vitro Tissue Culture of Pear Rootstocks OH×F51[J].Northern Horticulture,2014,38(18):107-109.
梨砧木OH×F51的离体组织培养研究
- Title:
- Research on in vitro Tissue Culture of Pear Rootstocks OH×F51
- 文章编号:
- 1001-0009（2014）18-0107-03
- Keywords:
- pear rootstock; OH×F51; shoot tips; leaves; in vitro tissue culture
- 分类号:
- Q 813.1+2
- 文献标志码:
- A
- 摘要:
- 以梨砧木OH×F51为试材，对其茎尖组织培养和叶片愈伤组织诱导进行了研究，以获得OH×F51离体培养的最佳途径。结果表明：适合OH×F51茎尖离体培养的培养基是MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+蔗糖30 g/L+琼脂6 g/L，有较高的成苗率；OH×F51叶片愈伤组织诱导的最佳培养基是NN69+TDZ 3.0 mg/L+IBA 1.0 mg/L+蔗糖30 g/L+琼脂6 g/L，叶片放置方式对愈伤组织诱导有一定影响，叶片的远轴面接触培养基，可以获得较高的出愈率。
- Abstract:
- Taking pear rootstock OH×F51 as material,the shoot tips tissue culture and leaf callus induction of OH×F51 were studied,in order to gain the optimum way of in vitro culture.The results showed that the suitable medium for shoot tips culture was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+sugar 30 g/L+agar 6 g/L，with a higher rate of seedling.The optimum medium for leaf callus induction of OH×F51 was NN69+TDZ 3.0 mg/L+IBA 1.0 mg/L+sugar 30 g/L+agar 6 g/L.The placing manner of leaf had a certain influence on the callus induction.The leaves which abaxial surface touched the medium had a higher rate of callus.
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备注/Memo
第一作者简介：宋健坤（1978-），男，博士，副教授，现主要从事梨育种等研究工作。E-mail:qausjk@126.com.
基金项目：青岛市科技计划基础研究资助项目（11-2-4-5-（2）-jch）；青岛农业大学高层次人才启动基金资助项目（630733）。
收稿日期：2014-04-18