[1]马诗钰,周兰英,蒲光,等.贴梗海棠花粉生活力与贮藏性研究[J].北方园艺,2014,38(16):81-85.
　MA Shi-yu,ZHOU Lan-ying,PU Guang-lan,et al.Study on Pollen Viability and Storage Characters of Chaenomeles speciosa[J].Northern Horticulture,2014,38(16):81-85.

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贴梗海棠花粉生活力与贮藏性研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷期数: 2014年16期页码: 81-85 栏目: 出版日期: 2014-08-30

Title:: Study on Pollen Viability and Storage Characters of Chaenomeles speciosa

文章编号:: 1001-0009(2014)16-0081-05

作者:: 马诗钰¹; 周兰英¹; 蒲光¹; 赖腾跃¹; 蔡利娟¹; 王波¹; 2; 1.四川农业大学林学院，四川雅安 625014；
2.四川工程职业技术学院建筑工程系，四川德阳 618000

Author(s):: MA Shi-yu¹; ZHOU Lan-ying¹; PU Guang-lan¹; LAI Teng-yue¹; CAI Li-juan¹; WANG Bo¹; 2; 1.College of Forestry,Sichuan Agricultural University,Ya’an,Sichuan 625014；
2.Department of Architectural Engineering,Sichuan Engineering Technical College,Deyang,Sichuan 618000

关键词:: 贴梗海棠; 花粉; 生活力; 贮藏; 萌发

Keywords:: Chaenomeles speciosa（Sweet）Nakai; pollen; viability; storage; germinationo germinate easily.If the concentration exceeded optimum capacity; germination rate of pollen would be restrained.Moreover; high concent

分类号:: s661.4

文献标志码:: A

摘要:: 以贴梗海棠初开花朵为试材，研究了次甲基蓝、TTC、I_2-KI、а-萘酚-联苯胺4种染色剂测定花粉生活力的效果，3种贮藏条件下（-20、4、25℃）花粉生活力的变化规律及离体培养时蔗糖、硼酸、温度对花粉萌发的影响和最佳培养基组合。结果表明：不同染色剂测定的花粉生活力差异显著，次甲基蓝（84.94%）最适合贴梗海棠花粉染色，I_2-KI（44.62%）不适合该种花粉染色。贮藏温度对花粉生活力影响显著，温度越低，贮藏时间越长，各贮藏条件下，花粉活力降到50%以下的时间分别为3 d(25℃)、10 d(4℃)、7 d(-20℃)，4℃贮藏条件最佳。在一定范围内，蔗糖、硼酸和温度能促进花粉萌发，超过范围后抑制萌发，且高浓度硼酸对萌发的抑制作用比低浓度硼酸强，单因素试验下最佳蔗糖、硼酸浓度和温度分别为10%、0.03%、20℃，正交实验花粉萌发的最佳组合为8%(蔗糖)+0.03%(硼酸)+20℃(温度)。

Abstract:: Taking young bloom Chaenomeles speciosa as experimental materials，four coloring agents (Methylene Blue,TTC,I_2-KI and alpha-naphthol benzidine) were used to measure pollen viability.Storage characters under three different temperature conditions (-20,4 and 25℃) and germination percentage under different concentrations of sucrose,boric acid and temperature were studied respectively.The results showed that pollen viability measured by different coloring agents showed significantly differences.Methylene Blue(84.94%) was the best choices for rapid determination,while the worst was I_2-KI(44.62%).Storage temperatures had remarkable effects on pollen viability,that was the lower the temperature,the longer the storage time.The storage time of pollen viability percentage under 50% were 3 days(25℃),10 days(4℃) and 7 days(-25℃),the optimum temperature for pollen storage was 4℃.Within certain limits,sucrose,boric acid and temperature could promote pollen tration of boric acid gave a severe inhibition on germination by contrast with low boric acid concentration.The optimal sucrose,boric acid concentration and temperature were 10%，0.03%，20℃ respectively during single factor experiment.The optimal portfolio of solid medium verified by orthogonal test was 8%(sucrose)+0.03%(boric acid)+20℃(temperature).

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备注/Memo

第一作者简介：马诗钰（1989-），女，硕士研究生，研究方向为林木遗传育种。E-mail：melishery1989@163.com.

责任作者：周兰英（1960-），女，教授，博士生导师，现主要从事林木遗传育种及园林植物与观赏园艺教学与科研工作。E-mail：949460359@qq.com.

更新日期/Last Update: 2014-09-26