[1]袁红梅,赵丽娟,郭文栋,等.小黑杨花粉总RNA提取方法的比较研究[J].北方园艺,2013,37(06):113-116.
　YUAN Hong-mei,ZHAO Li-juan,GUO Wen-dong,et al.Comparison and Analysis of Total RNA Extraction Methods from Populus simonii×P.nigra Pollen[J].Northern Horticulture,2013,37(06):113-116.
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小黑杨花粉总RNA提取方法的比较研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年06期 页码: 113-116 栏目: 出版日期: 2013-03-30
Title:
Comparison and Analysis of Total RNA Extraction Methods from Populus simonii×P.nigra Pollen
文章编号:
1001-0009(2013)06-0113-04
作者:
袁红梅1; 2; 赵丽娟3; 郭文栋4; 吴建忠2; 张利国2; 关凤芝2
1.东北林业大学 林木遗传育种国家重点实验室，黑龙江 哈尔滨 150040；
2.黑龙江省农业科学院 经济作物研究所，黑龙江 哈尔滨 150086；
3.黑龙江省农业科学院 作物育种研究所，黑龙江 哈尔滨 150086；
4.黑龙江省科学院 自然与生态研究所，黑龙江 哈尔滨 150040
Author(s):
YUAN Hong-mei1; 2; ZHAO Li-juan3; GUO Wen-dong4; WU Jian-zhong2; ZHANG Li-guo2; GUAN Feng-zhi2
1.State Key Laboratory of Tree Genetics and Breeding，Northeast Forestry University，Harbin，Heilongjiang 150040；
2.Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086；
3.Institute of Crop Breeding,Heilongjiang Academy of Agricultural Sciences，Harbin，Heilongjiang 150086;
4.Institute of Nature and Ecology,Heilongjiang Academy of Sciences，Harbin，Heilongjiang 150040
关键词:
小黑杨; 花粉; 总RNA提取; RT-PCR
Keywords:
Populus simonii×P.nigra; pollen; RNA extraction; RT-PCR
分类号:
S 792.11
文献标志码:
A
摘要:
以小黑杨（Populus simonii×P.nigra）成熟花粉为试材，分别采用传统CTAB法、柱式植物RNAout试剂盒法、改良CTAB法及SDS法4种方法提取小黑杨花粉总RNA;通过琼脂糖凝胶电泳、紫外分光光度计及RT-PCR检测，研究比较了4种方法的优劣。结果表明：SDS法提取的总RNA纯度高、完整性好，总RNA质量显著高于其它3种方法，且该方法具有步骤简单、提取时间短、成本低等优点，是一种适用于小黑杨花粉总RNA提取的比较理想的方法。
Abstract:
Taking the pollen of Populus simonii×P.nigra as material，four kinds of total RNA extraction methods (traditional CTAB method，RNAout kit，improved CTAB method and SDS method) were compared using agarose gel electrophoresis，spectrophotometry and RT-PCR method.The results showed that SDS method was suitable fortotal RNA with high purity and good integrity extraction from Populus simonii×P.nigra pollen.It had also the advantage of simplicity，less operation time and low cost.It was an ideal method for total RNA extraction from Populus simonii×P.nigra pollen.

参考文献/References:

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备注/Memo

第一作者简介：袁红梅（1979-），女，黑龙江依安人，博士，助理研究院，现主要从事作物遗传育种等研究工作。E-mail:yuanhm1979@163.com.

更新日期/Last Update: 2014-08-23